Effect of milk proteins on the behavior of a biosensor based on poly(allylamine) containing an osmium complex wired to redox enzymes: Part 2. Bienzymatic configuration

Four configurations of an enzyme electrode to assess lactate concentration in dairy products are studied. In these bienzymatic multilayer systems, the inner layer was constructed by crosslinking peroxidase with poly(allylamine) containing an osmium complex (OsPAA), and an outer layer containing lact...

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Detalles Bibliográficos
Autor principal: Cortón, E.
Otros Autores: Battaglini, Fernando
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 2001
Acceso en línea:Registro en Scopus
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Registro en la Biblioteca Digital
Aporte de:Registro referencial: Solicitar el recurso aquí
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100 1 |a Cortón, E. 
245 1 0 |a Effect of milk proteins on the behavior of a biosensor based on poly(allylamine) containing an osmium complex wired to redox enzymes: Part 2. Bienzymatic configuration 
260 |c 2001 
270 1 0 |m Battaglini, F.; Departamento de Química Inorgánica, Analítica and Química Física, INQUIMAE, Facultad de Ciencias Exactas and Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón. 2, 1428 Buenos Aires, Argentina; email: battagli@ql.fcen.uba.ar 
504 |a Collier, W.A., Janssen, D., Hart, A.L., (1996) Biosens. Bioelectron., 11, p. 1041 
504 |a Hajizadeh, K., Halsall, H.B., Heinemann, W.R., (1991) Talanta, 38, p. 37 
504 |a Kim, N., Haginoya, R., Karube, I., (1996) J. Food Sci., 61, p. 286 
504 |a Boehringer Mannheim, Enzymatic BioAnalysis, Catalog. no. 1112821 
504 |a Galbaén, J., Marcos, S., Castillo, J.R., (1993) Anal. Chem., 65, p. 3076 
504 |a Cortón, E., Bataglini, F., (2001) J. Electroanal. Chem., 511, p. 1 
504 |a Danilowicz, C., Cortón, E., Battaglini, F., (1998) J. Electroanal. Chem., 445, p. 89 
504 |a Danilowicz, C., Cortón, E., Battaglini, F., Calvo, E.J., (1998) Electrochim. Acta, 43, p. 3525 
504 |a Kulys, J.J., Pesliakinene, M.V., Samalius, A.S., (1981) Bioelectrochem. Bioeng., 8, p. 81 
504 |a Ohara, T., Vreeke, M., Battaglini, F., Heller, A., (1993) Electroanalysis, 5, p. 825 
504 |a Belay, A., Collins, A., Ruzgas, T., Kissinger, P.T., Gorton, L., Csöregi, E., (1999) J. Pharm. Biomed. Anal., 19, p. 93 
504 |a Kenausis, G., Chen, Q., Heller, A., (1997) Anal. Chem., 69, p. 1054 
506 |2 openaire  |e Política editorial 
520 3 |a Four configurations of an enzyme electrode to assess lactate concentration in dairy products are studied. In these bienzymatic multilayer systems, the inner layer was constructed by crosslinking peroxidase with poly(allylamine) containing an osmium complex (OsPAA), and an outer layer containing lactate oxidase (LOx) immobilized in polymeric matrices. When the hydrogen peroxide formed by the LOx layer reaches the inner layer, the electronic flow between the wired peroxidase and the electrode surface produces a cathodic current, proportional to lactate concentration. Parameters such as the effect of ionic strength, stability when the electrode is used as detector in a FIA system and reproducibility were examined. This arrangement allows working in undiluted milk samples. © 2001 Elsevier Science B.V.  |l eng 
536 |a Detalles de la financiación: International Foundation for Science 
536 |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas 
536 |a Detalles de la financiación: The International Foundation for Science and CONICET are acknowledged for financial support. We acknowledge Sandra Ruzal for the measurements of l -lactate made with the spectrophotometric method. 
593 |a Departamento de Química Biológica, Facultad de Ciencias Exactas and Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón. 2, 1428 Buenos Aires, Argentina 
593 |a Departamento de Química Inorgánica, Analítica and Química Física, INQUIMAE, Facultad de Ciencias Exactas and Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón. 2, 1428 Buenos Aires, Argentina 
690 1 0 |a BIOSENSORS 
690 1 0 |a CHRONOAMPEROMETRY 
690 1 0 |a CYCLIC VOLTAMMETRY 
690 1 0 |a FLOW INJECTION ANALYSIS 
690 1 0 |a REDOX ENZYMES 
690 1 0 |a CATHODES 
690 1 0 |a CROSSLINKING 
690 1 0 |a CYCLIC VOLTAMMETRY 
690 1 0 |a ELECTROCHEMICAL ELECTRODES 
690 1 0 |a ENZYME IMMOBILIZATION 
690 1 0 |a IONIC STRENGTH 
690 1 0 |a OSMIUM COMPOUNDS 
690 1 0 |a PROTEINS 
690 1 0 |a REDOX REACTIONS 
690 1 0 |a CATHODIC CURRENTS 
690 1 0 |a BIOSENSORS 
700 1 |a Battaglini, Fernando 
773 0 |d 2001  |g v. 511  |h pp. 8-12  |k n. 1-2  |p J. Electroanal. Chem.  |x 00220728  |w (AR-BaUEN)CENRE-102  |t Journal of Electroanalytical Chemistry 
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