Effect of milk proteins on the behavior of a biosensor based on poly(allylamine) containing an osmium complex wired to redox enzymes: Part 2. Bienzymatic configuration
Four configurations of an enzyme electrode to assess lactate concentration in dairy products are studied. In these bienzymatic multilayer systems, the inner layer was constructed by crosslinking peroxidase with poly(allylamine) containing an osmium complex (OsPAA), and an outer layer containing lact...
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Formato: | Capítulo de libro |
Lenguaje: | Inglés |
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2001
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Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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LEADER | 05080caa a22006617a 4500 | ||
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001 | PAPER-20582 | ||
003 | AR-BaUEN | ||
005 | 20241007100128.0 | ||
008 | 190411s2001 xx ||||fo|||| 00| 0 eng|d | ||
024 | 7 | |2 scopus |a 2-s2.0-0035928947 | |
040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
100 | 1 | |a Cortón, E. | |
245 | 1 | 0 | |a Effect of milk proteins on the behavior of a biosensor based on poly(allylamine) containing an osmium complex wired to redox enzymes: Part 2. Bienzymatic configuration |
260 | |c 2001 | ||
270 | 1 | 0 | |m Battaglini, F.; Departamento de Química Inorgánica, Analítica and Química Física, INQUIMAE, Facultad de Ciencias Exactas and Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón. 2, 1428 Buenos Aires, Argentina; email: battagli@ql.fcen.uba.ar |
504 | |a Collier, W.A., Janssen, D., Hart, A.L., (1996) Biosens. Bioelectron., 11, p. 1041 | ||
504 | |a Hajizadeh, K., Halsall, H.B., Heinemann, W.R., (1991) Talanta, 38, p. 37 | ||
504 | |a Kim, N., Haginoya, R., Karube, I., (1996) J. Food Sci., 61, p. 286 | ||
504 | |a Boehringer Mannheim, Enzymatic BioAnalysis, Catalog. no. 1112821 | ||
504 | |a Galbaén, J., Marcos, S., Castillo, J.R., (1993) Anal. Chem., 65, p. 3076 | ||
504 | |a Cortón, E., Bataglini, F., (2001) J. Electroanal. Chem., 511, p. 1 | ||
504 | |a Danilowicz, C., Cortón, E., Battaglini, F., (1998) J. Electroanal. Chem., 445, p. 89 | ||
504 | |a Danilowicz, C., Cortón, E., Battaglini, F., Calvo, E.J., (1998) Electrochim. Acta, 43, p. 3525 | ||
504 | |a Kulys, J.J., Pesliakinene, M.V., Samalius, A.S., (1981) Bioelectrochem. Bioeng., 8, p. 81 | ||
504 | |a Ohara, T., Vreeke, M., Battaglini, F., Heller, A., (1993) Electroanalysis, 5, p. 825 | ||
504 | |a Belay, A., Collins, A., Ruzgas, T., Kissinger, P.T., Gorton, L., Csöregi, E., (1999) J. Pharm. Biomed. Anal., 19, p. 93 | ||
504 | |a Kenausis, G., Chen, Q., Heller, A., (1997) Anal. Chem., 69, p. 1054 | ||
506 | |2 openaire |e Política editorial | ||
520 | 3 | |a Four configurations of an enzyme electrode to assess lactate concentration in dairy products are studied. In these bienzymatic multilayer systems, the inner layer was constructed by crosslinking peroxidase with poly(allylamine) containing an osmium complex (OsPAA), and an outer layer containing lactate oxidase (LOx) immobilized in polymeric matrices. When the hydrogen peroxide formed by the LOx layer reaches the inner layer, the electronic flow between the wired peroxidase and the electrode surface produces a cathodic current, proportional to lactate concentration. Parameters such as the effect of ionic strength, stability when the electrode is used as detector in a FIA system and reproducibility were examined. This arrangement allows working in undiluted milk samples. © 2001 Elsevier Science B.V. |l eng | |
536 | |a Detalles de la financiación: International Foundation for Science | ||
536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
536 | |a Detalles de la financiación: The International Foundation for Science and CONICET are acknowledged for financial support. We acknowledge Sandra Ruzal for the measurements of l -lactate made with the spectrophotometric method. | ||
593 | |a Departamento de Química Biológica, Facultad de Ciencias Exactas and Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón. 2, 1428 Buenos Aires, Argentina | ||
593 | |a Departamento de Química Inorgánica, Analítica and Química Física, INQUIMAE, Facultad de Ciencias Exactas and Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón. 2, 1428 Buenos Aires, Argentina | ||
690 | 1 | 0 | |a BIOSENSORS |
690 | 1 | 0 | |a CHRONOAMPEROMETRY |
690 | 1 | 0 | |a CYCLIC VOLTAMMETRY |
690 | 1 | 0 | |a FLOW INJECTION ANALYSIS |
690 | 1 | 0 | |a REDOX ENZYMES |
690 | 1 | 0 | |a CATHODES |
690 | 1 | 0 | |a CROSSLINKING |
690 | 1 | 0 | |a CYCLIC VOLTAMMETRY |
690 | 1 | 0 | |a ELECTROCHEMICAL ELECTRODES |
690 | 1 | 0 | |a ENZYME IMMOBILIZATION |
690 | 1 | 0 | |a IONIC STRENGTH |
690 | 1 | 0 | |a OSMIUM COMPOUNDS |
690 | 1 | 0 | |a PROTEINS |
690 | 1 | 0 | |a REDOX REACTIONS |
690 | 1 | 0 | |a CATHODIC CURRENTS |
690 | 1 | 0 | |a BIOSENSORS |
700 | 1 | |a Battaglini, Fernando | |
773 | 0 | |d 2001 |g v. 511 |h pp. 8-12 |k n. 1-2 |p J. Electroanal. Chem. |x 00220728 |w (AR-BaUEN)CENRE-102 |t Journal of Electroanalytical Chemistry | |
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856 | 4 | 0 | |u https://doi.org/10.1016/S0022-0728(01)00500-9 |x doi |y DOI |
856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_00220728_v511_n1-2_p8_Corton |x handle |y Handle |
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961 | |a paper_00220728_v511_n1-2_p8_Corton |b paper |c PE | ||
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