Direct molecular identification of Trypanosoma cruzi Discrete Typing Units in domestic and peridomestic Triatoma infestans and Triatoma sordida from the Argentine Chaco
We assessed the distribution of Trypanosoma cruzi Discrete Typing Units (DTUs) in domestic and peridomestic Triatoma infestans and Triatoma sordida specimens collected in a well-defined rural area in Pampa del Indio, northeastern Argentina. Microscopically-positive bugs were randomly selected with a...
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| Otros Autores: | , , , , , |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
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2012
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| Sumario: | We assessed the distribution of Trypanosoma cruzi Discrete Typing Units (DTUs) in domestic and peridomestic Triatoma infestans and Triatoma sordida specimens collected in a well-defined rural area in Pampa del Indio, northeastern Argentina. Microscopically-positive bugs were randomly selected with a multi-level sampling design, and DTUs were identified using direct PCR strategies. TcVI predominated in 61% of 69 T. infestans and in 56% of 9 T. sordida. TcV was the secondary DTU in T. infestans (16%) and was found in 1 T. sordida specimen (11%). Three T. sordida (33%) were found infected with TcI, a DTU also identified in local Didelphis albiventris opossums. Mixed DTU infections occurred rarely (5%) and were detected both directly from the bugs' rectal ampoule and parasite cultures. The identified DTUs and bug collection sites of T. infestans were significantly associated. Bugs infected with TcV were almost exclusively captured in domiciles whereas those with TcVI were found similarly in domiciles and peridomiciles. All mixed infections occurred in domiciles. TcV-infected bugs fed more often on humans than on dogs, whereas TcVI-infected bugs showed the reverse pattern. T. sordida is a probable sylvatic vector of TcI linked to D. albiventris, and could represent a secondary vector of TcVI and TcV in the domestic/peridomestic cycle. © Copyright © Cambridge University Press 2012. |
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| Bibliografía: | Alvarado-Otegui, J.A., Ceballos, L.A., Orozco, M.M., Enriquez, G.F., Cardinal, M.V., Schiman, A.G., Kitron, U., Gürtler, R.E., The sylvatic transmission cycle of trypanosoma cruzi in the humid chaco of argentina (2012) Acta Tropica, , in the Press Bar, M.E., Wisnivesky-Colli, C., Triatoma sordida Stål 1859 (Hemiptera, Reduviidae: Triatominae) in palms of Northeastern Argentina (2001) Memorias Do Instituto Oswaldo Cruz, 96, pp. 895-899 Bosseno, M.F., Yacsik, N., Vargas, F., Brenière, S.F., Selection of Trypanosoma cruzi clonal genotypes (Clonet 20 and 39) isolated from Bolivian triatomines following subculture in liquid medium (2000) Memorias Do Instituto Oswaldo Cruz, 95, pp. 601-607 Brenière, S.F., Bosseno, M.F., Noireau, F., Yacsik, N., Liegeard, P., Aznar, C., Hontebeyrie, M., Integrated study of a bolivian population infected by trypanosoma cruzi, the agent of chagas disease (2002) Memorias Do Instituto Oswaldo Cruz, 97, pp. 289-295 Brisse, S., Verhoef, J., Tibayrenc, M., Characterization of large and small subunit rRNA and mini-exon genes further supports the distinction of six Trypanosoma cruzi lineages (2001) International Journal for Parasitology, 31, pp. 1218-1226 Burgos, J.M., Altcheh, J., Bisio, M., Duffy, T., Valadares, H.M.S., Seidenstein, M.E., Piccinali, R., Schijman, A.G., Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations cuasing congenital Chagas disease (2007) International Journal for Parasitology, 37, pp. 1319-1327 Cardinal, M.V., Castañera, M.B., Lauricella, M.A., Cecere, M.C., Ceballos, A.L., Vazquez-Prokopec, G.M., Kitron, U., Gürtler, R.E., A prospective study of the effects of sustained vector surveillance following community-wide insecticide application on Trypanosoma cruzi of dogs and cats in rural northwestern Argentina (2006) American Journal of Tropical Medicine and Hygiene, 75, pp. 753-761 Cardinal, M.V., Lauricella, M.A., Ceballos, L.A., Lanati, L., Marcet, P.L., Levin, M.J., Kitron, U., Schijman, A.G., Molecular epidemiology of domestic and sylvatic Trypanosoma cruzi infection in rural northwestern Argentina (2008) International Journal for Parasitology, 38, pp. 1533-1543 Cardinal, M.V., Lauricella, M.A., Marcet, P.L., Orozco, M.M., Kitron, U., Gürtler, R.E., Impact of community-based vector control on house infestation and Trypanosoma cruzi infection in Triatoma infestans, dogs and cats in the Argentine Chaco (2007) Acta Tropica, 103, pp. 201-211 Ceballos, L.A., Cardinal, M.V., Vazquez-Prokopec, G.M., Lauricella, M.A., Orozco, M.M., Cortinas, R., Schijman, A.G., Gürtler, R.E., Long-term reduction of Trypanosoma cruzi infection in sylvatic mammals following deforestation and sustained vector surveillance in northwestern Argentina (2006) Acta Tropica, 98, pp. 286-296 Cohen, J.E., Gürtler, R.E., (2001) Science, 293, pp. 694-698. , Modeling household transmission of American Trypanosomiasis Cura, C.I., Lucero, R.H., Bisio, M., Oshiro, E., Formicelli, L.B., Burgos, J.M., Lejonas, S., Schijman, A.G., Trypanosoma cruzi Discrete Typing Units in Chagas disease patients from endemic and non endemic regions of Argentina (2012) Parasitology Cura, C.I., Mejía-Jaramillo, A.M., Duffy, T., Burgos, J.M., Rodriguero, M., Cardinal, M.V., Kjos, S., Schijman, A.G., Trypanosoma cruzi i genotypes in different geographical regions and transmission cycles based on a microsatellite motif of the intergenic spacer of spliced-leader genes (2010) International Journal for Parasitology, 40, pp. 1599-1607 De Luca D'Oro, G.M., Gardenal, C.N., Perret, B., Crisci, J.V., Montamat, E.E., Genetic structure of Trypanosoma cruzi populations from Argentina estimated from enzyme polymorphism (1993) Parasitology, 107, pp. 405-410 Diosque, P., Barnabe, C., Padilla, A.M., Marco, J.D., Cardozo, R.M., Cimino, R.O., Nasser, J.R., Basombrio, M.A., Multilocus enzyme electrophoresis analysis of Trypanosoma cruzi isolates from a geographically restricted endemic area for Chagas' disease in Argentina (2003) International Journal for Parasitology, 33, pp. 997-1003 Diosque, P., Padilla, A.M., Cimino, R.O., Marino Cardozo, R., Sanchez Negrette, O., Marco, J.D., Zacca, R., Basombrío, M.A., Chagas disease in rural areas of Chaco province, Argentina: Epidemiologic survey in humans, reservoirs, and vectors (2004) American Journal for Tropical Medicine and Hygiene, 71, pp. 590-593 Diotaiuti, L., Pereira, A.S., Loiola, C.F., Fernandes, A.J., Schofield, J.C., Dujardin, J.P., Dias, J.C., Chiari, E., Inter-relation of sylvatic and domestic transmission of Trypanosoma cruzi in areas with and without domestic vectorial transmission in Minas Gerais, Brazil (1995) Memorias Do Instituto Oswaldo Cruz, 90, pp. 443-448 Gurevitz, J.M., Ceballos, L.A., Gaspe, M.S., Alvarado Otegui, J.A., Enríquez, G.F., Kitron, U., Gürtler, R.E., Factors affecting infestation by Triatoma infestans in a rural area of the humid Chaco in Argentina: A multi-model inference approach (2011) PLoS Neglected Tropical Diseases, 5, pp. e1349. , 10.1371/journal.pntd.0001349 Gürtler, R.E., Ceballos, L.A., Ordóñez Krasnowski, P.C., Lanati, L.A., Stariolo, R., Kitron, U., Strong host-feeding preferences of the vector Triatoma infestans modified by vector density: Implications for the epidemiology of Chagas Disease (2009) PLoS Neglected Tropical Diseases, 3, pp. e447. , 10.1371/journal.pntd.0000447 Gürtler, R.E., Cecere, M.C., Lauricella, M.A., Cardinal, M.V., Kitron, U., Cohen, J.E., Domestic dogs and cats as sources of Trypanosoma cruzi infection in rural northwestern Argentina (2007) Parasitology, 134, pp. 69-82 Gürtler, R.E., Kitron, U., Cecere, M.C., Segura, E.L., Cohen, J.E., Sustainable vector control and management of Chagas disease in the Gran Chaco (2007) Argentina. Proceedings of the National Academy of Sciences, USA, 104, pp. 16194-16199 Herrera, C., Bargues, M.D., Fajardo, A., Montilla, M., Triana, O., Vallejo, G.A., Guhl, F., Identifying four Trypanosoma cruzi i isolate haplotypes from different geographic regions in Colombia (2007) Infection, Genetics and Evolution, 7, pp. 535-539 Higo, H., Miura, S., Horio, M., Mimori, T., Hamano, S., Agatsuma, T., Yanagi, T., Himeno, K., Genotypic variation among lineages of Trypanosoma cruzi and its geographic aspects (2004) Parasitology International, 53, pp. 337-344 Laurent, J.P., Barnabé, C., Quesney, V., Noel, S., Tibayrenc, M., Impact of clonal evolution on the biological diversity of Trypanosoma cruzi (1997) Parasitology, 114, pp. 213-218 Lauricella, M.A., Stariolo, R.L., Riarte, A.R., Segura, E.L., Gürtler, R.E., Distribution and pathogenicity of Trypanosoma cruzi isolated from peridomestic populations of Triatoma infestans and Triatoma guasayana from rural western Argentina (2005) Memorias Do Instituto Oswaldo Cruz, 100, pp. 123-129 Llewellyn, M.S., Miles, M.A., Carrasco, H.J., Lewis, M.D., Yeo, M., Vargas, J., Torrico, F., Gaunt, M.W., Genome-scale multilocus microsatellite typing of Trypanosoma cruzi discrete typing unit i reveals phylogeographic structure and specific genotypes linked to human infection (2009) PLoS Pathogens, 5, pp. e1000410 Llewellyn, M.S., Rivett-Carnac, J.B., Fitzpatrick, S., Lewis, M.D., Yeo, M., Gaunt, M.W., Miles, M.A., Extraordinary Trypanosoma cruzi diversity within single mammalian reservoir hosts implies a mechanism of diversifying selection (2011) International Journal for Parasitology, 41, pp. 609-614 Lewis, M.D., Ma, J., Yeo, M., Carrasco, H.J., Llewellyn, M.S., Miles, M.A., Genotyping of Trypanosoma cruzi: Systematic selection of assays allowing rapid and accurate discrimination of all known lineages (2009) American Journal of Tropical Medicine and Hygiene, 81, pp. 1041-1049 Loza-Murguía, M., Noireau, F., Vectorial capacity of Triatoma guasayana (Wygodzinsky & Abalos) (Hemiptera: Reduviidae) compared with two other species of epidemic importance (2010) Neotropical Entomology, 39, pp. 799-809 MacEdo, A.M., Pena, S.D., Genetic variability of Trypanosoma cruzi: Implications for the pathogenesis of Chagas disease (1998) Parasitology Today, 14, pp. 119-123 Marcet, P.L., Duffy, T., Cardinal, M.V., Burgos, J.M., Lauricella, M.A., Levin, M.J., Kitron, U., Schijman, A.G., PCR-based screening and lineage identification of Trypanosoma cruzi directly from faecal samples of triatomine bugs from northwestern Argentina (2006) Parasitology, 132, pp. 57-65 Miles, M.A., Llewellyn, M.S., Lewis, M.D., Yeo, M., Baleela, R., Fitzpatrick, S., Gaunt, M.W., Mauricio, I.L., The molecular epidemiology and phylogeography of Trypanosoma cruzi and parallel research on Leishmania: Iooking back and to the future (2009) Parasitology, 136, pp. 1509-1528 Montamat, E.E., Arauzo, S., Cazzulo, J.J., Subias, E., Characterization by electrophoretic zymograms of 19 Trypanosoma cruzi clones derived from two chronic chagasic patients (1987) Comparative Biochemistry and Physiology, 87, pp. 417-422 Noireau, F., Brenière, F., Ordoñez, J., Cardozo, L., Morochi, W., Gutierrez, T., Bosseno, M.F., Wisnivesky-Colli, C., Low probability of transmission of trypanosoma cruzi to humans by domiciliary triatoma sordida in bolivia (1997) Transactions of the Royal Society of Tropical Medicine and Hygiene, 91, pp. 653-656 Schofield, C.J., Lehane, M.J., McEwan, P., Catalá, S.S., Gorla, D.E., Dispersive flight by triatoma sordida (1991) Transactions of the Royal Society of Tropical Medicine and Hygiene, 85, pp. 676-678 Schweigmann, N.J., Pietrokovsky, S., Bottazzi, V., Conti, O., Bujas, M.A., Wisnivesky-Colli, C., Estudio de la prevalencia de infección por Trypanosoma cruzi en zarigüeyas (Didelphis albiventris) en Santiago del Estero, Argentina (1999) Revista Panamericana de Salud Pública, 6, pp. 371-377 (2007) Stata Statistical Software: Release 10.0, , StataCorp Stata Corporation, College Station, TX, USA Tibayrenc, M., Genetic epidemiology of parasitic protozoa and other infectious agents: The need for an integrated approach (1998) International Journal for Parasitology, 28, pp. 85-104 Vazquez-Prokopec, G.M., Ceballos, L.A., Marcet, P.L., Cecere, M.C., Cardinal, M.V., Kitron, U., Gürtler, R.E., Seasonal variations in active dispersal of natural populations of triatoma infestans in rural north-western argentina (2006) Medical and Veterinary Entomology, 20, pp. 273-279 Wisnivesky-Colli, C., Schweigmann, N.J., Alberti, A., Pietrokovsky, S.M., Conti, O., Montoya, S., Riarte, A., Rivas, C., Sylvatic american trypanosomiasis in argentina trypanosoma cruzi infection in mammals from the chaco forest in santiago del estero (1992) Transactions of the Royal Society of Tropical Medicine and Hygiene, 86, pp. 38-41 (2007) Report of the Scientific Working Group on Chagas Disease, , World Health Organization TDR/SWH/09 World Health Organization, Geneva, Switzerland Yeo, M., Acosta, N., Llewellyn, M., Sánchez, H., Adamson, S., Miles, G.A.J., López, E., Miles, M.A., Origins of Chagas disease: Didelphis species are natural hosts of Trypanosoma cruzi i and armadillos hosts of Trypanosoma cruzi II, including hybrids (2005) International Journal for Parasitology, 35, pp. 225-233 Yeo, M., Lewis, M.D., Carrasco, H.J., Acosta, N., Llewellyn, M., Aldo, S., Vera De Costa, V., Miles, M.A., Resolution of multiclonal infections of Trypanosoma cruzi from naturally infected triatomine bugs and from experimentally infected mice by direct plating on a sensitive solid medium (2007) International Journal for Parasitology, 37, pp. 111-120 Zingales, B., Andrade, S.G., Briones, M.R.S., Campbe, D.A., Chiari, E., Fernandes, O., Guhl, F., Schijman, A.G., A new consensus for Trypanosoma cruzi intraspecific nomenclature: Second revision meeting recommends TcI to TcVI (2009) Memorias Do Instituto Oswaldo Cruz, 104, pp. 1051-1054 |
| ISSN: | 00311820 |
| DOI: | 10.1017/S0031182012000856 |