New human isolates of Trypanosoma cruzi confirm the predominance of hybrid lineages in domestic transmission cycle of the Argentinean Chaco
Trypanosoma cruzi, the etiological agent of Chagas disease, was initially classified into 6 Discrete Typing Units (DTUs). The hybrid DTUs TcV and TcVI are the most frequent in domestic transmission cycles throughout the Southern Cone countries of South America. Here, we genotyped parasite isolates f...
| Autor principal: | |
|---|---|
| Otros Autores: | , , , , |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier B.V.
2018
|
| Materias: | |
| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| Sumario: | Trypanosoma cruzi, the etiological agent of Chagas disease, was initially classified into 6 Discrete Typing Units (DTUs). The hybrid DTUs TcV and TcVI are the most frequent in domestic transmission cycles throughout the Southern Cone countries of South America. Here, we genotyped parasite isolates from human residents in Pampa del Indio municipality, Chaco, to further characterize the structure of T. cruzi populations, and to assess the degree of overlapping between the domestic and sylvatic transmission cycles. Artificial xenodiagnostic tests were performed to blood samples from 125 T. cruzi-seropositive people (age range, 3–70 years) who represented 14.3% of all seropositive residents identified. Parasites were obtained from feces of T. cruzi-infected Triatoma infestans examined 30 or 60 days after blood-feeding, and grown in vitro. The cultured parasites were genotyped by means of two PCR-based protocols. DTUs were determined from 39 (31%) patients residing in 28 dwellings. The only DTUs identified were TcV (92%) and TcVI (8–36%). Households with more than one parasite isolate consistently displayed the same DTU. Further sequencing of a fragment of the TcMK gene from selected samples argue against the occurrence of mixed TcV-TcVI infections in the study population. Sequencing data revealed an unexpected degree of genetic variability within TcV including two apparently robust subgroups of isolates. Our results for human residents confirm the predominance of hybrid lineages (TcV and to a much lesser extent TcVI) and the absence of sylvatic genotypes (TcI and TcIII) in (peri)domestic transmission cycles in the Argentinean Chaco area. 245 words. © 2018 Elsevier B.V. |
|---|---|
| Bibliografía: | Alvarado-Otegui, J.A., Ceballos, L.A., Orozco, M.M., Enriquez, G.F., Cardinal, M.V., Cura, C.I., Schijman, A.G., Gürtler, R.E., The sylvatic transmission cycle of Trypanosoma cruzi in a rural area in the humid Chaco of Argentina (2012) Acta Trop., 124, pp. 79-86 Arenas, M., Campos, R., Coronado, X., Ortiz, S., Solari, A., Trypanosoma cruzi Genotypes of Insect Vectors and patients with Chagas of Chile Studied by Means of Cytochrome b Gene Sequencing, Minicircle Hybridization, and Nuclear Gene Polymorphisms (2012) Vector-Borne Zoonotic Dis., 12, pp. 196-205 Argibay, H.D., Orozco, M.M., Cardinal, M.V., Rinas, M.A., Arnaiz, M., Mena Segura, C., Gürtler, R.E., First finding of Trypanosoma cruzi II in vampire bats from a district free of domestic vector-borne transmission in Northeastern Argentina (2016) Parasitology, 143, pp. 1358-1368 Ault, S.K., Chagas disease and neglected diseases: changing poverty and exclusion, in: Organización Panamericana de la Salud, Fundación Mundo Sano (Eds.), La enfermedad de chagas a la puerta de los 100 años de conocimiento de una endemia americana ancestral (2007), pp. 13-18. , (Buenos Aires, Argentina); Bontempi, I.A., Bizai, M.L., Ortiz, S., Manattini, S., Fabbro, D., Solari, A., Diez, C., Simple methodology to directly genotype Trypanosoma cruzi discrete typing units in single and mixed infections from human blood samples (2016) Infect. Genet. Evol., 43, pp. 123-129 Bosseno, M.F., Yacsik, N., Vargas, F., Brenière, S.F., Selection of Trypanosoma cruzi clonal genotypes (clonet 20 and 39) isolated from Bolivian triatomines following subculture in liquid medium (2000) Mem. Inst. Oswaldo Cruz, 95, pp. 601-607 Brenière, S.F., Waleckx, E., Barnabé, C., Over six Thousand Trypanosoma cruzi Strains Classified into Discrete Typing units (DTUs): Attempt at an Inventory (2016) PLoS Negl. Trop. Dis., 10 Brisse, S., Barnabé, C., Tibayrenc, M., Identification of six Trypanosoma cruzi phylogenetic lineages by random amplified polymorphic DNA and multilocus enzyme electrophoresis (2000) Int. J. Parasitol., 30, pp. 35-44 Browne, A.J., Guerra, C.A., Alves, R.V., da Costa, V.M., Wilson, A.L., Pigott, D.M., Hay, S.I., Moyes, C.L., The contemporary distribution of Trypanosoma cruzi infection in humans, alternative hosts and vectors (2017) Sci. Data, 4 Bua, J., Volta, B.J., Perrone, A.E., Scollo, K., Velázquez, E.B., Ruiz, A.M., De Rissio, A.M., Cardoni, R.L., How to Improve the early Diagnosis of Trypanosoma cruzi Infection: Relationship between Validated conventional Diagnosis and Quantitative DNA Amplification in Congenitally Infected Children (2013) PLoS Negl. Trop. Dis., 7 Burgos, J.M., Altcheh, J., Bisio, M., Duffy, T., Valadares, H.M.S., Seidenstein, M.E., Piccinali, R.V., Schijman, A.G., Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations causing congenital Chagas disease (2007) Int. J. Parasitol., 37, pp. 1319-1327 Burgos, J.M., Diez, M., Vigliano, C., Bisio, M., Risso, M.G., Duffy, T., Cura, C.I., Schijman, A.G., Molecular Identification of Trypanosoma cruzi Discrete Typing Units in End-Stage Chronic Chagas Heart Disease and Reactivation after Heart Transplantation (2010) Clin. Infect. Dis., 51, pp. 485-495 Buscaglia, C.A., Di Noia, J.M., Trypanosoma cruzi clonal diversity and the epidemiology of Chagas' disease (2003) Microbes Infect., 5, pp. 419-427 Cardinal, M.V., Lauricella, M.A., Ceballos, L.A., Lanati, L.A., Marcet, P.L., Levin, M.J., Kitron, U., Schijman, A.G., Molecular epidemiology of domestic and sylvatic Trypanosoma cruzi infection in rural northwestern Argentina (2008) Int. J. Parasitol., 38, pp. 1533-1543 Cosentino, R.O., Agüero, F., A simple Strain Typing Assay for Trypanosoma cruzi: Discrimination of Major Evolutionary Lineages from a Single Amplification Product (2012) PLoS Negl. Trop. Dis., 6 Cura, C.I., Lucero, R.H., Bisio, M., Oshiro, E., Formichelli, L.B., Burgos, J.M., Lejona, S., Schijman, A.G., Trypanosoma cruzi Discrete Typing Units in Chagas disease patients from endemic and non endemic regions of Argentina (2012) Parasitology, 139, pp. 516-521 Del Puerto, R., Nishizawa, J.E., Kikuchi, M., Iihoshi, N., Roca, Y., Avilas, C., Gianella, A., Hirayama, K., Lineage analysis of circulating Trypanosoma cruzi parasites and their association with clinical forms of Chagas disease in Bolivia (2010) PLoS Negl. Trop. Dis., 4 Del Puerto, F., Sánchez, Z., Nara, E., Meza, G., Paredes, B., Ferreira, E., Russomando, G., Trypanosoma cruzi lineages detected in congenitally infected infants and Triatoma infestans from the same disease-endemic region under entomologic surveillance in Paraguay (2010) Am. J. Trop. Med. Hyg., 82, pp. 386-390 Diez, C., Lorenz, V., Ortiz, S., Gonzalez, V., Racca, A., Bontempi, I.A., Manattini, S., Marcipar, I., Genotyping of Trypanosoma cruzi sublineage in human samples from a north-East Argentina area by hybridization with DNA probes and specific polymerase chain reaction (PCR) (2010) Am. J. Trop. Med. Hyg., 82, pp. 67-73 Diosque, P., Barnabé, C., Padilla, A.M., Marco, J.D., Cardozo, R.M., Cimino, R.O., Nasser, J.R., Basombrío, M.A., Multilocus enzyme electrophoresis analysis of Trypanosoma cruzi isolates from a geographically restricted endemic area for Chagas' disease in Argentina (2003) Int. J. Parasitol., 33, pp. 997-1003 Enriquez, G.F., Cardinal, M.V., Orozco, M.M., Lanati, L.A., Schijman, A.G., Gürtler, R.E., Discrete typing units of Trypanosoma cruzi identified in rural dogs and cats in the humid Argentinean Chaco (2013) Parasitology, 140, pp. 303-308 Enriquez, G.F., Bua, J., Orozco, M.M., Wirth, S., Schijman, A.G., Gürtler, R.E., Cardinal, M.V., High levels of Trypanosoma cruzi DNA determined by qPCR and infectiousness to Triatoma infestans support dogs and cats are major sources of parasites for domestic transmission (2014) Infect. Genet. Evol., 25, pp. 36-43 Enriquez, G.F., Garbossa, G., Macchiaverna, N.P., Argibay, H.D., Bua, J., Gürtler, R.E., Cardinal, M.V., Is the infectiousness of dogs naturally infected with Trypanosoma cruzi associated with poly-parasitism? (2016) Vet. Parasitol., 223, pp. 186-194 Gaspe, M.S., Provecho, Y.M., Cardinal, M.V., del Pilar Fernández, M., Gürtler, R.E., Ecological and Sociodemographic Determinants of House Infestation by Triatoma infestans in Indigenous Communities of the argentine Chaco (2015) PLoS Negl. Trop. Dis., 9, pp. 1-26 Gurevitz, J.M., Gaspe, M.S., Enriquez, G.F., Provecho, Y.M., Kitron, U., Gürtler, R.E., Intensified Surveillance and Insecticide-based Control of the Chagas Disease Vector Triatoma infestans in the Argentinean Chaco (2013) PLoS Negl. Trop. Dis., 7 Higo, H., Miura, S., Agatsuma, T., Mimori, T., Yanagi, T., Iwagami, M., de Arias, A.R., Himeno, K., Identification of Trypanosoma cruzi sublineages by the simple method of single-stranded conformation DNA polymorphism (SSCP) (2007) Parasitol. Res., 100, pp. 1023-1031 Hotez, P.J., Bottazzi, M.E., Franco-Paredes, C., Ault, S.K., Periago, M.R., The neglected tropical diseases of Latin America and the Caribbean: a review of disease burden and distribution and a roadmap for control and elimination (2008) PLoS Negl. Trop. Dis. Kumar, S., Stecher, G., Tamura, K., MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for bigger Datasets (2016) Mol. Biol. Evol., 33, pp. 1870-1874 Lauricella, M.A., Stariolo, R., Riarte, A.R., Segura, E.L., Gürtler, R.E., Distribution and pathogenicity of Trypanosoma cruzi isolated from peridomestic populations of Triatoma infestans and Triatoma guasayana from rural Western Argentina (2005) Mem. Inst. Oswaldo Cruz, 100, pp. 123-129 Lewis, M.D., Ma, J., Yeo, M., Carrasco, H.J., Llewellyn, M.S., Miles, M.A., Genotyping of Trypanosoma cruzi: Systematic selection of assays allowing rapid and accurate discrimination of all known lineages (2009) Am. J. Trop. Med. Hyg., 81, pp. 1041-1049 Lewis, M.D., Llewellyn, M.S., Yeo, M., Acosta, N., Gaunt, M.W., Miles, M.A., Recent, Independent and Anthropogenic Origins of Trypanosoma cruzi Hybrids (2011) PLoS Negl. Trop. Dis., 5, pp. 1-13 Lucero, R.H., Brusés, B.L., Cura, C.I., Formichelli, L.B., Juiz, N., Fernández, G.J., Bisio, M., Schijman, A.G., Chagas' disease in Aboriginal and Creole communities from the Gran Chaco Region of Argentina: Seroprevalence and molecular parasitological characterization (2016) Infect. Genet. Evol., 41, pp. 84-92 Macchiaverna, N.P., Gaspe, M.S., Enriquez, G.F., Tomassone, L., Gürtler, R.E., Cardinal, M.V., Trypanosoma cruzi infection in Triatoma sordida before and after community-wide residual insecticide spraying in the Argentinean Chaco (2015) Acta Trop., 143, pp. 97-102 Macedo, A.M., Pena, S.D.J., Genetic Variability of Trypanosoma cruzi: Implications for the Pathogenesis of Chagas Disease (1998) Parasitol. Today, 14, pp. 119-124 Maffey, L., Cardinal, M.V., Ordóñez-Krasnowski, P.C., Lanati, L.A., Lauricella, M.A., Schijman, A.G., Gürtler, R.E., Direct molecular identification of Trypanosoma cruzi discrete typing units in domestic and peridomestic Triatoma infestans and Triatoma sordida from the argentine Chaco (2012) Parasitology, 139, pp. 1570-1579 Marcili, A., Valente, V.C., da Silva Valente, S.A., Junqueira, A.C.V., da Silva, F.M., Pinto, A.Y.D.N., Naiff, R.D., Teixeira, M.M.G., Trypanosoma cruzi in Brazilian Amazonia: Lineages TCI and TCIIa in wild primates, Rhodnius spp. and in humans with Chagas disease associated with oral transmission (2009) Int. J. Parasitol., 39, pp. 615-623 Martinez-Perez, A., Poveda, C., Ramírez, J.D., Norman, F., Gironés, N., Guhl, F., Monge-Maillo, B., López-Vélez, R., Prevalence of Trypanosoma cruzi's Discrete Typing Units in a cohort of Latin American migrants in Spain (2016) Acta Trop., 157, pp. 145-150 Martins, K., de Mesquita Andrade, C., Noronhan Barbosa-Silva, A., Barbosa do Nascimento, G., Chiari, E., da Cunha Galvão, L.M., Jácome da Câmara, A.C., Trypanosoma cruzi III causing the indeterminate form of Chagas disease in a semi-arid region of Brazil (2015) Int. J. Infect. Dis., 39, pp. 68-75 Miles, M.A., Feliciangeli, M.D., Rojas De Arias, A., American trypanosomiasis (Chaga's disease) and the role of molecular epideiology in guiding control strategies (2003) Br. Med. J., 326, pp. 1444-1448 Monje-Rumi, M.M., Pérez Brandán, C., Gil, J.F., D'Amato, A.M.A., Ragone, P.G., Lauthier, J.J., Tomasini, N., Diosque, P., Benznidazole treatment in chronic children infected with Trypanosoma cruzi: Serological and molecular follow-up of patients and identification of Discrete Typing Units (2013) Acta Trop., 128, pp. 130-136 Monje-Rumi, M.M., Brandán, C.P., Ragone, P.G., Tomasini, N., Lauthier, J.J., Alberti D'Amato, A.M., Cimino, R.O., Pérez Brandán, C., Trypanosoma cruzi diversity in the Gran Chaco: mixed infections and differential host distribution of TcV and TcVI (2015) Infect. Genet. Evol., 29, pp. 53-59 Noireau, F., Diosque, P., Jansen, A.M., Trypanosoma cruzi: Adaptation to its vectors and its hosts (2009) Vet. Res., 40, p. 26 Orozco, M.M., Enriquez, G.F., Alvarado-Otegui, J.A., Cardinal, M.V., Schijman, A.G., Kitron, U., Gürtler, R.E., New sylvatic hosts of Trypanosoma cruzi and their reservoir competence in the humid Chaco of Argentina: a longitudinal study (2013) Am. J. Trop. Med. Hyg., 88, pp. 872-882 Pena, S.D.J., Machado, C.R., Macedo, A.M., Trypanosoma cruzi: ancestral genomes and population structure (2009) Mem. Inst. Oswaldo Cruz, 104, pp. 108-114 Provecho, Y.M., Gaspe, M.S., Fernández, M.D.P., Enriquez, G.F., Weinberg, D., Gürtler, R.E., The peri-urban interface and house infestation with Triatoma infestans in the argentine Chaco: an underreported process? (2014) Mem. Inst. Oswaldo Cruz, 109, pp. 923-934 Provecho, Y.M., Gaspe, M.S., Del Pilar Fernández, M., Gürtler, R.E., Byrd, J., House Reinfestation with Triatoma infestans (Hemiptera: Reduviidae) after Community-Wide Spraying with Insecticides in the argentine Chaco: a Multifactorial Process (2017) J. Med. Entomol., 54, pp. 646-657 Ramírez, J.D., Hernández, C., Trypanosoma cruzi I: Towards the need of genetic subdivision?, Part II (2018) Acta Trop., 184, pp. 53-58 Ramírez, J.C., Cura, C.I., da Cruz Moreira, O., Lages-Silva, E., Juiz, N., Velázquez, E.B., Ramírez, J.D., Schijman, A.G., Analytical Validation of Quantitative Real-Time PCR Methods for Quantification of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease patients (2015) J. Mol. Diagnostics, 17, pp. 605-615 Ramírez, J.C., Torres, C., Curto, M.D.L.A., Schijman, A.G., New insights into Trypanosoma cruzi evolution, genotyping and molecular diagnostics from satellite DNA sequence analysis (2017) PLoS Negl. Trop. Dis., 11 Sartor, P., Colaianni, I., Cardinal, M.V., Bua, J., Freilij, H., Gürtler, R.E., Improving access to Chagas disease diagnosis and etiologic treatment in remote rural communities of the argentine Chaco through strengthened primary health care and broad social participation (2017) PLoS Negl. Trop. Dis., 11, pp. 1-18 Solari, A., Campillay, R., Ortiz, S., Wallace, A., Identification of Trypanosoma cruzi Genotypes Circulating in Chilean Chagasic patients (2001) Exp. Parasitol., 97, pp. 226-233 Sturm, N.R., Vargas, N.S., Westenberger, S.J., Zingales, B., Campbell, D.A., Evidence for multiple hybrid groups in Trypanosoma cruzi (2003) Int. J. Parasitol., 33, pp. 269-279 (2017), https://doi.org/10.1016/j.actatropica.2016.11.033, Tavares de Oliveira, M., Tupinambá Branquinho, R., Diniz Alessio, G., Campos Mello, C.G., Nogueira-de-Paiva, N.C., Martins Carneiro, C., de Ornelas Toledo, M.J., Barbosa Reis, A., Assis Martins Martins-Filho, O., de Lana, M. TcI, TcII and TcVI Trypanosoma cruzi samples from Chagas disease patients with distinct clinical forms and critical analysis of in vitro and in vivo behavior, response to treatment and infection evolution in murine model. Acta Trop. 167, 108–120. doi:; Virreira, M., Alonso-Vega, C., Carlier, Y., Solano, M., Svoboda, M., Jijena, J., Brutus, L., Torrico, F., Congenital Chagas Disease in Bolivia is not associated with DNA polymorphism of Trypanosoma cruzi (2006) Am. J. Trop. Med. Hyg., 75, pp. 871-879 Westenberger, S.J., Sturm, N.R., Campbell, D.A., Sánchez, H., Adamson, S., Miles, G.A.J., López, E., Sturm, N.R., Two Hybridization events Define the Population Structure of Trypanosoma cruzi (2005) Genetics, 171, pp. 527 LP-543 World Health Organization, Chagas disease in Latin America: an epidemiological update based on 2010 estimates (2015) Wkly Epidemiol. Rec., 33-44 Yeo, M., Acosta, N., Llewellyn, M.S., Sánchez, H., Adamson, S., Miles, G.A.J., López, E., Miles, M.A., Origins of Chagas disease: Didelphis species are natural hosts of Trypanosoma cruzi I and armadillos hosts of Trypanosoma cruzi II, including hybrids (2005) Int. J. Parasitol., 35, pp. 225-233 Yeo, M., Mauricio, I.L., Messenger, L.A., Lewis, M.D., Llewellyn, M.S., Acosta, N., Bhattacharyya, T., Miles, M.A., Multilocus sequence typing MLST for lineage assignment and high resolution diversity studies in Trypanosoma cruzi (2011) PLoS Negl. Trop. Dis., 5 Zingales, B., Andrade, S.G., Briones, M.R.S., Campbell, D.A., Chiari, E., Fernandes, O., Guhl, F., Schijman, A.G., A New Consensus for Trypanosoma cruzi Intraspecific no- Menclature: Second Revision Meeting Recommends TcI to TcVI [WWW Document]. Mem. Inst. Oswaldo Cruz (2009) URL, , http://www.scielo.br/pdf/mioc/v104n7/21.pdf Zingales, B., Miles, M.A., Campbell, D.A., Tibayrenc, M., Macedo, A.M., Teixeira, M.M.G., Schijman, A.G., Sturm, N.R., The revised Trypanosoma cruzi subspecific nomenclature: Rationale, epidemiological relevance and research applications (2012) Infect. Genet. Evol., 12, pp. 240-253 |
| ISSN: | 15671348 |
| DOI: | 10.1016/j.meegid.2018.10.001 |