Digital simulation of homogeneous enzyme kinetics for amperometric redox-enzyme electrodes
An explicit point method is described for digital simulation of redox-enzyme catalysis and diffusion at soluble-enzyme electrodes. Two experimental conditions (chronopotentiometry and open-circuit potential decay) were simulated for a two-substrate enzyme (glucose oxidase) catalyzing the anaerobic o...
| Autor principal: | |
|---|---|
| Otros Autores: | |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
1992
|
| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| Sumario: | An explicit point method is described for digital simulation of redox-enzyme catalysis and diffusion at soluble-enzyme electrodes. Two experimental conditions (chronopotentiometry and open-circuit potential decay) were simulated for a two-substrate enzyme (glucose oxidase) catalyzing the anaerobic oxidation of glucose, mediated by a soluble redox couple. Transient and steady-state responses are compared to experimental data and to predictions of the analytical solutions for selected cases. Simulated steady-state concentration profiles near the electrode surface are analyzed for different values of Ks/Cs and KO/CO. The validity of linear Lineweaver-Burk plots for the amperometric response of enzyme electrodes is discussed. © 1992. |
|---|---|
| Bibliografía: | Lowe, (1985) Biosensors, 1, p. 3 Weibel, Bright, (1971) J. Biol. Chem., 246, p. 2734 Duke, Weibel, Page, Bulgrin, Luthy, (1969) J. Am. Chem. Soc., 91, p. 3904 Gibson, Swoboda, Massey, (1964) J. Biol. Chem., 239, p. 3927 Hill, Walton, Marine natural products: parguerol, deoxyparguerol, and isoparguerol. New brominated diterpenes with modified pimarane skeletons from the sea hare Aplysia dactylomela (1982) Journal of the American Chemical Society, 104, p. 6415 Bright, Porter, (1975) The Enzymes, 12, p. 421. , P. Boyer, Academic Press, New York Fersht, (1985) Enzyme, Structure and Mechanism, , Freeman, London Liaudet, Battaglini, Calvo, (1990) J. Electroanal. Chem., 293, p. 55 Swoboda, Massey, (1965) J. Biol. Chem., 240, p. 2209 Britz, (1988) Digital Simulation in Electrochemistry, , 2nd edn., Springer-Verlag, Berlin Feldberg, (1969) Electroanal. Chem., 3, p. 199 Mell, Maloy, (1975) Anal. Chem., 47, p. 299 Britz, (1988) Electroanal. Chem., 240, p. 17 Britz, (1987) Anal. Chim. Acta, 193, p. 277 Battaglini, Calvo, (1990) J. Electroanal. Chem., 280, p. 443 Delahay, Steihl, (1952) J. Am. Chem. Soc., 74, p. 3500 Miller, (1952) J. Am. Chem. Soc., 74, p. 4130 Saveant, Vianello, (1965) Electrochim. Acta, 10, p. 905 Bard, Faulkner, (1980) Electrochemical Methods, p. 456. , Wiley, New York MacDonald, (1977) Transient Techniques in Electrochemisty, p. 49. , Plenum Press, New York Bartlett, Whitaker, (1987) J. Electroanal. Chem., 224, p. 27 Bourdillon, Hervaganet, Thomas, (1985) Biotechnol. Bioeng., 27, p. 1619 Tatsuma, Okawa, Watanabe, (1989) Anal. Chem., 81, p. 2352 Taniguchi, Miyamoto, Tomimura, Hawkridge, (1988) J. Electroanal. Chem., 240, p. 333 Albery, Bartlett, Driscoll, Lenox, (1990), un, published, results, ; P.N. Bartlett, personal communication |
| ISSN: | 00032670 |
| DOI: | 10.1016/0003-2670(92)85208-N |