Standardization of fixation, processing and staining methods for the central nervous system of vertebrates
This paper reports the standardization of methods used for processing and embedding various vertebrate brains of different sizes in paraffin. Other technical details developed for avoiding frequent difficulties arising during laboratory routine are also reported. Some modifications of the Nissl and...
| Autores principales: | , , , |
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| Formato: | Artículo publishedVersion |
| Publicado: |
1996
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_03279545_v20_n3_p265_AldanaMarcos https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_03279545_v20_n3_p265_AldanaMarcos_oai |
| Aporte de: |
| Sumario: | This paper reports the standardization of methods used for processing and embedding various vertebrate brains of different sizes in paraffin. Other technical details developed for avoiding frequent difficulties arising during laboratory routine are also reported. Some modifications of the Nissl and Klüver-Barrera staining methods are proposed. These modifications include: 1) a Nissl stain solution with a rapid and efficient action with easier differentiation; 2) the use of a cheap microwave oven for the Klüver-Barrera stain. These procedures have the advantage of permitting Nissl and Klüver-Barrera staining of nervous tissue in about five and fifteen minutes respectively. The proposed procedures have been tested in brains obtained from fish, amphibians, reptiles and mammals of different body sizes. They are the result of our long experience in preparing slides for comparative studies. Serial sections of excellent quality were regularly obtained in all the specimens studied. These standardized methods, being simple and quick, are recommended for routine use in neurobiological laboratories. |
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