Effects of radioiodination on hormone binding ability
This paper presents the effects of 125I labelling on human chorionic gonadotropin binding ability. The results obtained showed a marked difference in the half-life of the specific radioactivity of the radiolabelled hormone comparing the one obtained by "self-displacement" analysis and that...
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1985
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10799893_v5_n5-6_p349_Radicella http://hdl.handle.net/20.500.12110/paper_10799893_v5_n5-6_p349_Radicella |
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paper:paper_10799893_v5_n5-6_p349_Radicella2023-06-08T16:05:39Z Effects of radioiodination on hormone binding ability Radicella, Juan Pablo Calvo, Juan Carlos Charreau, Eduardo Hernán radioisotope chorionic gonadotropin i 125 endocrine system hormone binding human human cell methodology priority journal radioiodination Animal Binding Sites Chorionic Gonadotropin Chromatography, Gel Half-Life In Vitro Iodine Radioisotopes Kinetics Male Molecular Weight Rats Testis This paper presents the effects of 125I labelling on human chorionic gonadotropin binding ability. The results obtained showed a marked difference in the half-life of the specific radioactivity of the radiolabelled hormone comparing the one obtained by "self-displacement" analysis and that calculated considering the half-life of the radioactive isotope (60 days for 125I). The decrease in the hormone half-life (t1/2= 3.10 days) was demonstrated by binding experiments performed at various days after hormone labelling. The affinity constant (Ka) and the number of binding sites obtained after Scatchard analysis using rat testes as receptor source, remained constant when the "self-displacement" specific radioactivity was used while they varied significantly if the 60 days half-life was considered for the data processing. The importance of the labelled hormone degradation as a possible cause of the decrease in the binding ability is also described. © 1985 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted. Fil:Radicella, J.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Charreau, E.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1985 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10799893_v5_n5-6_p349_Radicella http://hdl.handle.net/20.500.12110/paper_10799893_v5_n5-6_p349_Radicella |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
radioisotope chorionic gonadotropin i 125 endocrine system hormone binding human human cell methodology priority journal radioiodination Animal Binding Sites Chorionic Gonadotropin Chromatography, Gel Half-Life In Vitro Iodine Radioisotopes Kinetics Male Molecular Weight Rats Testis |
| spellingShingle |
radioisotope chorionic gonadotropin i 125 endocrine system hormone binding human human cell methodology priority journal radioiodination Animal Binding Sites Chorionic Gonadotropin Chromatography, Gel Half-Life In Vitro Iodine Radioisotopes Kinetics Male Molecular Weight Rats Testis Radicella, Juan Pablo Calvo, Juan Carlos Charreau, Eduardo Hernán Effects of radioiodination on hormone binding ability |
| topic_facet |
radioisotope chorionic gonadotropin i 125 endocrine system hormone binding human human cell methodology priority journal radioiodination Animal Binding Sites Chorionic Gonadotropin Chromatography, Gel Half-Life In Vitro Iodine Radioisotopes Kinetics Male Molecular Weight Rats Testis |
| description |
This paper presents the effects of 125I labelling on human chorionic gonadotropin binding ability. The results obtained showed a marked difference in the half-life of the specific radioactivity of the radiolabelled hormone comparing the one obtained by "self-displacement" analysis and that calculated considering the half-life of the radioactive isotope (60 days for 125I). The decrease in the hormone half-life (t1/2= 3.10 days) was demonstrated by binding experiments performed at various days after hormone labelling. The affinity constant (Ka) and the number of binding sites obtained after Scatchard analysis using rat testes as receptor source, remained constant when the "self-displacement" specific radioactivity was used while they varied significantly if the 60 days half-life was considered for the data processing. The importance of the labelled hormone degradation as a possible cause of the decrease in the binding ability is also described. © 1985 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted. |
| author |
Radicella, Juan Pablo Calvo, Juan Carlos Charreau, Eduardo Hernán |
| author_facet |
Radicella, Juan Pablo Calvo, Juan Carlos Charreau, Eduardo Hernán |
| author_sort |
Radicella, Juan Pablo |
| title |
Effects of radioiodination on hormone binding ability |
| title_short |
Effects of radioiodination on hormone binding ability |
| title_full |
Effects of radioiodination on hormone binding ability |
| title_fullStr |
Effects of radioiodination on hormone binding ability |
| title_full_unstemmed |
Effects of radioiodination on hormone binding ability |
| title_sort |
effects of radioiodination on hormone binding ability |
| publishDate |
1985 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10799893_v5_n5-6_p349_Radicella http://hdl.handle.net/20.500.12110/paper_10799893_v5_n5-6_p349_Radicella |
| work_keys_str_mv |
AT radicellajuanpablo effectsofradioiodinationonhormonebindingability AT calvojuancarlos effectsofradioiodinationonhormonebindingability AT charreaueduardohernan effectsofradioiodinationonhormonebindingability |
| _version_ |
1768543909449826304 |