| Sumario: | The occurrence of a two-step mechanism for the synthesis of a protein bound glucan in potato tuber was confirmed based on a different behaviour of the engaged enzymes towards 1,5-D-gluconolactone. The absence of [14C]erythritol after Smith periodate degradation of the labelled product synthesized during both steps in the presence of 1,5-D-gluconolactone indicated a lack of 1,4 glucosidic linkages. On the contrary, the first step of the reaction, i.e., the formation of a peptidyl-glucose linkage, was not affected by the lactone. These results reinforce the hypothesis of a common mechanism for glucan chain elongation. © 1979.
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