A simple method for taking photographs of histological sections without using neither photographic camera nor microscope

A simple photographic method for histological sections is described. This is a contact method which does not require the use of photographic camera. It is very similar to the contact print method used routinely in photographic laboratories. It is performed by placing the slides with the histological...

Descripción completa

Detalles Bibliográficos
Autores principales: Carmanchahi, P.D., Aldana Marcos, H.J., Ferrari, C.C., Affanni, J.M.
Formato: JOUR
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03279545_v22_n3_p207_Carmanchahi
Aporte de:
id todo:paper_03279545_v22_n3_p207_Carmanchahi
record_format dspace
spelling todo:paper_03279545_v22_n3_p207_Carmanchahi2023-10-03T15:25:09Z A simple method for taking photographs of histological sections without using neither photographic camera nor microscope Carmanchahi, P.D. Aldana Marcos, H.J. Ferrari, C.C. Affanni, J.M. Histology Immunohistochemistry Neuroanatomy Photography Retrograde neuronal tracing article emulsion film histology human illumination immunohistochemistry neuroanatomy nonhuman photography Histological Techniques Photomicrography A simple photographic method for histological sections is described. This is a contact method which does not require the use of photographic camera. It is very similar to the contact print method used routinely in photographic laboratories. It is performed by placing the slides with the histological section; coverslip downwards, over the emulsion of the negative film. Then, they are illuminated with a common photographic enlarger during a brief period of time. Thus, a negative contact print of the section is obtained. This technique yields photographs of high quality focus, sharply contrasted with great detail. These results may also be obtained with low- contrast stained sections. This method is most suitable for photography of large or medium-sized histological sections (macros). This is often the case of neuroanatomical and immunohistochemical studies. Our method, being simple and quick, is recommended for routine use in laboratories. Fil:Carmanchahi, P.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Aldana Marcos, H.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ferrari, C.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03279545_v22_n3_p207_Carmanchahi
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Histology
Immunohistochemistry
Neuroanatomy
Photography
Retrograde neuronal tracing
article
emulsion
film
histology
human
illumination
immunohistochemistry
neuroanatomy
nonhuman
photography
Histological Techniques
Photomicrography
spellingShingle Histology
Immunohistochemistry
Neuroanatomy
Photography
Retrograde neuronal tracing
article
emulsion
film
histology
human
illumination
immunohistochemistry
neuroanatomy
nonhuman
photography
Histological Techniques
Photomicrography
Carmanchahi, P.D.
Aldana Marcos, H.J.
Ferrari, C.C.
Affanni, J.M.
A simple method for taking photographs of histological sections without using neither photographic camera nor microscope
topic_facet Histology
Immunohistochemistry
Neuroanatomy
Photography
Retrograde neuronal tracing
article
emulsion
film
histology
human
illumination
immunohistochemistry
neuroanatomy
nonhuman
photography
Histological Techniques
Photomicrography
description A simple photographic method for histological sections is described. This is a contact method which does not require the use of photographic camera. It is very similar to the contact print method used routinely in photographic laboratories. It is performed by placing the slides with the histological section; coverslip downwards, over the emulsion of the negative film. Then, they are illuminated with a common photographic enlarger during a brief period of time. Thus, a negative contact print of the section is obtained. This technique yields photographs of high quality focus, sharply contrasted with great detail. These results may also be obtained with low- contrast stained sections. This method is most suitable for photography of large or medium-sized histological sections (macros). This is often the case of neuroanatomical and immunohistochemical studies. Our method, being simple and quick, is recommended for routine use in laboratories.
format JOUR
author Carmanchahi, P.D.
Aldana Marcos, H.J.
Ferrari, C.C.
Affanni, J.M.
author_facet Carmanchahi, P.D.
Aldana Marcos, H.J.
Ferrari, C.C.
Affanni, J.M.
author_sort Carmanchahi, P.D.
title A simple method for taking photographs of histological sections without using neither photographic camera nor microscope
title_short A simple method for taking photographs of histological sections without using neither photographic camera nor microscope
title_full A simple method for taking photographs of histological sections without using neither photographic camera nor microscope
title_fullStr A simple method for taking photographs of histological sections without using neither photographic camera nor microscope
title_full_unstemmed A simple method for taking photographs of histological sections without using neither photographic camera nor microscope
title_sort simple method for taking photographs of histological sections without using neither photographic camera nor microscope
url http://hdl.handle.net/20.500.12110/paper_03279545_v22_n3_p207_Carmanchahi
work_keys_str_mv AT carmanchahipd asimplemethodfortakingphotographsofhistologicalsectionswithoutusingneitherphotographiccameranormicroscope
AT aldanamarcoshj asimplemethodfortakingphotographsofhistologicalsectionswithoutusingneitherphotographiccameranormicroscope
AT ferraricc asimplemethodfortakingphotographsofhistologicalsectionswithoutusingneitherphotographiccameranormicroscope
AT affannijm asimplemethodfortakingphotographsofhistologicalsectionswithoutusingneitherphotographiccameranormicroscope
AT carmanchahipd simplemethodfortakingphotographsofhistologicalsectionswithoutusingneitherphotographiccameranormicroscope
AT aldanamarcoshj simplemethodfortakingphotographsofhistologicalsectionswithoutusingneitherphotographiccameranormicroscope
AT ferraricc simplemethodfortakingphotographsofhistologicalsectionswithoutusingneitherphotographiccameranormicroscope
AT affannijm simplemethodfortakingphotographsofhistologicalsectionswithoutusingneitherphotographiccameranormicroscope
_version_ 1807314661550325760