Layer-by-layer self-assembly of glucose oxidase with a poly(allylamine)ferrocene redox mediator

We report the redox mediation of glucose oxidase (GOx) in a self-assembled structure of cationic poly-(allylamine) modified by ferrocene (PAA-Fc) and anionic GOx deposited electrostatically layer-by-layer on negatively charged alkanethiol-modified Au surfaces. Successive PAA-Fc and GOx layers were d...

Descripción completa

Detalles Bibliográficos
Autores principales:	Hodak, J., Etchenique, R., Calvo, E.J., Singhal, K., Bartlett, P.N.
Formato:	JOUR
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_07437463_v13_n10_p2708_Hodak
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_07437463_v13_n10_p2708_Hodak
record_format	dspace
spelling	todo:paper_07437463_v13_n10_p2708_Hodak2023-10-03T15:38:30Z Layer-by-layer self-assembly of glucose oxidase with a poly(allylamine)ferrocene redox mediator Hodak, J. Etchenique, R. Calvo, E.J. Singhal, K. Bartlett, P.N. We report the redox mediation of glucose oxidase (GOx) in a self-assembled structure of cationic poly-(allylamine) modified by ferrocene (PAA-Fc) and anionic GOx deposited electrostatically layer-by-layer on negatively charged alkanethiol-modified Au surfaces. Successive PAA-Fc and GOx layers were deposited by alternate immersion of the thiol-modified Au in the respective polyelectrolyte and enzyme solutions. The uptake of thiol, redox polymer, and GOx on the surface was monitored by quartz crystal microbalance. Cyclic voltammetry shows nearly ideal surface waves of ferrocene in the polymer with charge independent of sweep rate; the redox surface concentration was obtained from integration of the ferrocene/ferricinium voltammetric peaks. The redox charge increases in step with the number of PAA-Fc layers deposited. Enzyme catalysis for the oxidation of β-D-glucose was achieved with a multilayer PAA-Fc/GOx assembly, with each GOx layer contributing equally to the catalytic response. Only a small fraction of the active assembled GOx molecules are "electrically wired" by the ferrocene polymer although all of the enzyme could be oxidized by soluble ferrocenesulfonate when added to solution. Fil:Hodak, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Etchenique, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calvo, E.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_07437463_v13_n10_p2708_Hodak
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
description	We report the redox mediation of glucose oxidase (GOx) in a self-assembled structure of cationic poly-(allylamine) modified by ferrocene (PAA-Fc) and anionic GOx deposited electrostatically layer-by-layer on negatively charged alkanethiol-modified Au surfaces. Successive PAA-Fc and GOx layers were deposited by alternate immersion of the thiol-modified Au in the respective polyelectrolyte and enzyme solutions. The uptake of thiol, redox polymer, and GOx on the surface was monitored by quartz crystal microbalance. Cyclic voltammetry shows nearly ideal surface waves of ferrocene in the polymer with charge independent of sweep rate; the redox surface concentration was obtained from integration of the ferrocene/ferricinium voltammetric peaks. The redox charge increases in step with the number of PAA-Fc layers deposited. Enzyme catalysis for the oxidation of β-D-glucose was achieved with a multilayer PAA-Fc/GOx assembly, with each GOx layer contributing equally to the catalytic response. Only a small fraction of the active assembled GOx molecules are "electrically wired" by the ferrocene polymer although all of the enzyme could be oxidized by soluble ferrocenesulfonate when added to solution.
format	JOUR
author	Hodak, J. Etchenique, R. Calvo, E.J. Singhal, K. Bartlett, P.N.
spellingShingle	Hodak, J. Etchenique, R. Calvo, E.J. Singhal, K. Bartlett, P.N. Layer-by-layer self-assembly of glucose oxidase with a poly(allylamine)ferrocene redox mediator
author_facet	Hodak, J. Etchenique, R. Calvo, E.J. Singhal, K. Bartlett, P.N.
author_sort	Hodak, J.
title	Layer-by-layer self-assembly of glucose oxidase with a poly(allylamine)ferrocene redox mediator
title_short	Layer-by-layer self-assembly of glucose oxidase with a poly(allylamine)ferrocene redox mediator
title_full	Layer-by-layer self-assembly of glucose oxidase with a poly(allylamine)ferrocene redox mediator
title_fullStr	Layer-by-layer self-assembly of glucose oxidase with a poly(allylamine)ferrocene redox mediator
title_full_unstemmed	Layer-by-layer self-assembly of glucose oxidase with a poly(allylamine)ferrocene redox mediator
title_sort	layer-by-layer self-assembly of glucose oxidase with a poly(allylamine)ferrocene redox mediator
url	http://hdl.handle.net/20.500.12110/paper_07437463_v13_n10_p2708_Hodak
work_keys_str_mv	AT hodakj layerbylayerselfassemblyofglucoseoxidasewithapolyallylamineferroceneredoxmediator AT etcheniquer layerbylayerselfassemblyofglucoseoxidasewithapolyallylamineferroceneredoxmediator AT calvoej layerbylayerselfassemblyofglucoseoxidasewithapolyallylamineferroceneredoxmediator AT singhalk layerbylayerselfassemblyofglucoseoxidasewithapolyallylamineferroceneredoxmediator AT bartlettpn layerbylayerselfassemblyofglucoseoxidasewithapolyallylamineferroceneredoxmediator
_version_	1807316493736607744

Layer-by-layer self-assembly of glucose oxidase with a poly(allylamine)ferrocene redox mediator

Ejemplares similares