Characterization of the regulatory subunit of Yarrowia lipolytica cAMP - dependent protein kinase evidences of a monomeric protein

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cAMP-dependent protein kinase [PKA] catalytic [C] and regulatory [R] subunits from Yarrowia lipolytica are encoded by single genes, TPK1 and RKA1, respectively. Here we performed the heterologous expression, purification and characterization of the R subunit from Y. lipolytica yeast cells, and explo...

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Detalles Bibliográficos
Otros Autores: Kronberg, María Florencia, Giacometti, Romina, Ruiz Herrera, José, Passeron, Susana
Formato: Artículo
Lenguaje:Inglés
Materias:
AUTOPHOSPHORYLATION
PKA
REGULATORY SUBUNIT
YARROWIA LIPOLYTICA
CYCLIC AMP DEPENDENT PROTEIN KINASE
HOLOENZYME
MONOMER
AMINO TERMINAL SEQUENCE
CENTRIFUGATION
CONTROLLED STUDY
DIMERIZATION
GEL FILTRATION
HYDRODYNAMICS
IN VIVO STUDY
MASS SPECTROMETRY
NONHUMAN
PROTEIN ANALYSIS
PROTEIN EXPRESSION
PROTEIN INTERACTION
PROTEIN PHOSPHORYLATION
PROTEIN PURIFICATION
AMINO ACID SEQUENCE
CLONING, MOLECULAR
CYCLIC AMP-DEPENDENT PROTEIN KINASES
GENE EXPRESSION
HOLOENZYMES
MOLECULAR SEQUENCE DATA
PHOSPHORYLATION
PROTEIN MULTIMERIZATION
PROTEIN SUBUNITS
RECOMBINANT PROTEINS
YARROWIA
Acceso en línea:http://ri.agro.uba.ar/files/intranet/articulo/2011Kronberg.pdf
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Aporte de:Registro referencial: Solicitar el recurso aquí
Biblioteca Central (FAUBA) de Universidad de Buenos Aires
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