New human isolates of Trypanosoma cruzi confirm the predominance of hybrid lineages in domestic transmission cycle of the Argentinean Chaco
Trypanosoma cruzi, the etiological agent of Chagas disease, was initially classified into 6 Discrete Typing Units (DTUs). The hybrid DTUs TcV and TcVI are the most frequent in domestic transmission cycles throughout the Southern Cone countries of South America. Here, we genotyped parasite isolates f...
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| Otros Autores: | , , , , |
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Elsevier B.V.
2018
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| 024 | 7 | |2 scopus |a 2-s2.0-85055101980 | |
| 030 | |a IGENC | ||
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 100 | 1 | |a Macchiaverna, N.P. | |
| 245 | 1 | 0 | |a New human isolates of Trypanosoma cruzi confirm the predominance of hybrid lineages in domestic transmission cycle of the Argentinean Chaco |
| 260 | |b Elsevier B.V. |c 2018 | ||
| 270 | 1 | 0 | |m Cardinal, M.V.; Laboratory of Eco-Epidemiology, Faculty of Exact and Natural Sciences, University of Buenos Aires, Ciudad Universitaria, 1428. Buenos Aires, Argentina; email: mvcardinal@ege.fcen.uba.ar |
| 504 | |a Alvarado-Otegui, J.A., Ceballos, L.A., Orozco, M.M., Enriquez, G.F., Cardinal, M.V., Cura, C.I., Schijman, A.G., Gürtler, R.E., The sylvatic transmission cycle of Trypanosoma cruzi in a rural area in the humid Chaco of Argentina (2012) Acta Trop., 124, pp. 79-86 | ||
| 504 | |a Arenas, M., Campos, R., Coronado, X., Ortiz, S., Solari, A., Trypanosoma cruzi Genotypes of Insect Vectors and patients with Chagas of Chile Studied by Means of Cytochrome b Gene Sequencing, Minicircle Hybridization, and Nuclear Gene Polymorphisms (2012) Vector-Borne Zoonotic Dis., 12, pp. 196-205 | ||
| 504 | |a Argibay, H.D., Orozco, M.M., Cardinal, M.V., Rinas, M.A., Arnaiz, M., Mena Segura, C., Gürtler, R.E., First finding of Trypanosoma cruzi II in vampire bats from a district free of domestic vector-borne transmission in Northeastern Argentina (2016) Parasitology, 143, pp. 1358-1368 | ||
| 504 | |a Ault, S.K., Chagas disease and neglected diseases: changing poverty and exclusion, in: Organización Panamericana de la Salud, Fundación Mundo Sano (Eds.), La enfermedad de chagas a la puerta de los 100 años de conocimiento de una endemia americana ancestral (2007), pp. 13-18. , (Buenos Aires, Argentina); Bontempi, I.A., Bizai, M.L., Ortiz, S., Manattini, S., Fabbro, D., Solari, A., Diez, C., Simple methodology to directly genotype Trypanosoma cruzi discrete typing units in single and mixed infections from human blood samples (2016) Infect. Genet. Evol., 43, pp. 123-129 | ||
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| 504 | |a Enriquez, G.F., Garbossa, G., Macchiaverna, N.P., Argibay, H.D., Bua, J., Gürtler, R.E., Cardinal, M.V., Is the infectiousness of dogs naturally infected with Trypanosoma cruzi associated with poly-parasitism? (2016) Vet. Parasitol., 223, pp. 186-194 | ||
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| 506 | |2 openaire |e Política editorial | ||
| 520 | 3 | |a Trypanosoma cruzi, the etiological agent of Chagas disease, was initially classified into 6 Discrete Typing Units (DTUs). The hybrid DTUs TcV and TcVI are the most frequent in domestic transmission cycles throughout the Southern Cone countries of South America. Here, we genotyped parasite isolates from human residents in Pampa del Indio municipality, Chaco, to further characterize the structure of T. cruzi populations, and to assess the degree of overlapping between the domestic and sylvatic transmission cycles. Artificial xenodiagnostic tests were performed to blood samples from 125 T. cruzi-seropositive people (age range, 3–70 years) who represented 14.3% of all seropositive residents identified. Parasites were obtained from feces of T. cruzi-infected Triatoma infestans examined 30 or 60 days after blood-feeding, and grown in vitro. The cultured parasites were genotyped by means of two PCR-based protocols. DTUs were determined from 39 (31%) patients residing in 28 dwellings. The only DTUs identified were TcV (92%) and TcVI (8–36%). Households with more than one parasite isolate consistently displayed the same DTU. Further sequencing of a fragment of the TcMK gene from selected samples argue against the occurrence of mixed TcV-TcVI infections in the study population. Sequencing data revealed an unexpected degree of genetic variability within TcV including two apparently robust subgroups of isolates. Our results for human residents confirm the predominance of hybrid lineages (TcV and to a much lesser extent TcVI) and the absence of sylvatic genotypes (TcI and TcIII) in (peri)domestic transmission cycles in the Argentinean Chaco area. 245 words. © 2018 Elsevier B.V. |l eng | |
| 536 | |a Detalles de la financiación: Fundación Bunge y Born | ||
| 536 | |a Detalles de la financiación: Universidad de Buenos Aires, 20020130100843BA, UBACYT 20020100100944 | ||
| 536 | |a Detalles de la financiación: Agencia Nacional de Promoción Científica y Tecnológica, PICT 2014-2661 | ||
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas, PIP No 11220110101146 | ||
| 536 | |a Detalles de la financiación: Parts of this work were supported by grants from ANPCyT ( PICT 2014-2661 ), CONICET (PIP No 11220110101146 ), University of Buenos Aires ( UBACYT 20020100100944 and 20020130100843BA ), and Fundación Bunge & Born. The funders had no role in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript. Appendix A | ||
| 593 | |a Laboratorio de Eco-Epidemiología, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires e Instituto de Ecología, Genética y Evolución de Buenos Aires (IEGEBA), UBA-CONICET, Capital Federal, Argentina | ||
| 593 | |a Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (IIB-INTECh), Universidad Nacional de San Martín (UNSAM) and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina | ||
| 651 | 4 | |a CHACO | |
| 651 | 4 | |a ARGENTINA | |
| 690 | 1 | 0 | |a DTU |
| 690 | 1 | 0 | |a HUMAN |
| 690 | 1 | 0 | |a ISOLATES |
| 690 | 1 | 0 | |a MOLECULAR EPIDEMIOLOGY |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a ADOLESCENT |
| 690 | 1 | 0 | |a ADULT |
| 690 | 1 | 0 | |a AGED |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a CHILD |
| 690 | 1 | 0 | |a CONTROLLED STUDY |
| 690 | 1 | 0 | |a FEMALE |
| 690 | 1 | 0 | |a HUMAN |
| 690 | 1 | 0 | |a MAJOR CLINICAL STUDY |
| 690 | 1 | 0 | |a MALE |
| 690 | 1 | 0 | |a MIXED INFECTION |
| 690 | 1 | 0 | |a MOLECULAR EPIDEMIOLOGY |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a PARASITE ISOLATION |
| 690 | 1 | 0 | |a PARASITE TRANSMISSION |
| 690 | 1 | 0 | |a POLYMERASE CHAIN REACTION |
| 690 | 1 | 0 | |a POPULATION STRUCTURE |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a RURAL AREA |
| 690 | 1 | 0 | |a SINGLE NUCLEOTIDE POLYMORPHISM |
| 690 | 1 | 0 | |a TRIATOMA INFESTANS |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a XENODIAGNOSIS |
| 700 | 1 | |a Enriquez, G.F. | |
| 700 | 1 | |a Buscaglia, C.A. | |
| 700 | 1 | |a Balouz, V. | |
| 700 | 1 | |a Gürtler, Ricardo Esteban | |
| 700 | 1 | |a Cardinal, M.V. | |
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