Design of a fluorescence technique using double laser pulse excitation for the measurement of molecular Brownian dynamics
A complete analysis of a novel method to evaluate the rotational diffusion time of fluorescent molecules is presented. It is based on the study of the total fluorescence produced after excitation with two consecutive short laser pulses. Expressions for the total fluorescence energy emitted in this c...
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
1997
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 04045caa a22005897a 4500 | ||
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| 001 | PAPER-3147 | ||
| 003 | AR-BaUEN | ||
| 005 | 20250311083841.0 | ||
| 008 | 190411s1997 xx ||||fo|||| 00| 0 eng|d | ||
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| 030 | |a OPCOB | ||
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 100 | 1 | |a Torga, J.R. | |
| 245 | 1 | 0 | |a Design of a fluorescence technique using double laser pulse excitation for the measurement of molecular Brownian dynamics |
| 260 | |b Elsevier |c 1997 | ||
| 270 | 1 | 0 | |m Torga, J.R.; Departamento de Fisica, Fac. de Ciencias Exactas y Naturales, Ciudad Universitaria, Pabellón 1, 1428 Buenos Aires, Argentina; email: torga@df.uba.ar |
| 504 | |a Lakowicz, J.R., (1986) Principles of Fluorescence Spectroscopy, , Plenum, New York | ||
| 504 | |a Perrin, F., (1929) Ann. Phys., 12, p. 169 | ||
| 504 | |a Perrin, F., (1936) Acta Phys. Pol., 5, p. 335 | ||
| 504 | |a Jablonski, A., (1960) Bull. Acad. Pol. Sci., 8, p. 259 | ||
| 504 | |a Weber, G., (1989) J. Phys. Chem., 93, p. 6069 | ||
| 504 | |a Chuang, T.J., Eisenthal, B.K., (1972) J. Chem. Phys., 57, p. 5094 | ||
| 504 | |a Tao, T., (1969) Biopolymers, 8, p. 609 | ||
| 504 | |a Kawski, A., (1993) Crit. Rev. Anal. Chem., 23, p. 459 | ||
| 504 | |a Spaans, E.A.J., Van Exter, M.P., Lagendijk, A., (1990) Optics Comm., 76, p. 376 | ||
| 504 | |a Shank, C.V., Ippen, E.P., (1975) Appl. Phys. Lett., 26, p. 62 | ||
| 504 | |a Waldeck, D.H., Fleming, G.R., (1981) J. Phys. Chem., 85, p. 2614 | ||
| 506 | |2 openaire |e Política editorial | ||
| 520 | 3 | |a A complete analysis of a novel method to evaluate the rotational diffusion time of fluorescent molecules is presented. It is based on the study of the total fluorescence produced after excitation with two consecutive short laser pulses. Expressions for the total fluorescence energy emitted in this case are derived, which would allow the evaluation of the rotational diffusion time. The main advantages of the proposed method are its simple setup, a time resolution only limited by the exciting laser pulsewidth, and the fact that it does not require fast time response detectors. © 1997 Elsevier Science B.V. |l eng | |
| 536 | |a Detalles de la financiación: Universidad de Buenos Aires | ||
| 536 | |a Detalles de la financiación: EX285 | ||
| 536 | |a Detalles de la financiación: This work was supportedb y the Universidad de Buenos Aires (PMA Program and grant EX285) and a grant from Fundacidn Antorchas. We thank P.F. Aramendia and M. Negri for helpful discussions on several aspects of this work. | ||
| 593 | |a Departamento de Fisica, Fac. de Ciencias Exactas y Naturales, Ciudad Universitaria, Pabellón 1, 1428 Buenos Aires, Argentina | ||
| 593 | |a Departamento de Matemática, Fac. de Ciencias Exactas y Naturales, Ciudad Universitaria, Pabellón 1, 1428 Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a BROWNIAN DYNAMICS |
| 690 | 1 | 0 | |a FLUORESCENCE ANISOTROPY |
| 690 | 1 | 0 | |a ROTATIONAL DEPOLARIZATION |
| 690 | 1 | 0 | |a BROWNIAN MOVEMENT |
| 690 | 1 | 0 | |a FLUORESCENCE |
| 690 | 1 | 0 | |a MOLECULAR DYNAMICS |
| 690 | 1 | 0 | |a ROTATIONAL DEPOLARIZATION |
| 690 | 1 | 0 | |a LASER PULSES |
| 700 | 1 | |a Etcheverry, Javier Ignacio | |
| 700 | 1 | |a Marconi, M.C. | |
| 773 | 0 | |d Elsevier, 1997 |g v. 143 |h pp. 230-234 |k n. 4-6 |p Opt Commun |x 00304018 |w (AR-BaUEN)CENRE-276 |t Optics Communications | |
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| 963 | |a VARI | ||