HPV 16 mutations associated to oral cancer
Abstract: Human Papillomavirus (HPV) is the most frequent sexually transmitted infection (STI) worldwide and despite the well-established role of HPV in cervical cancer, new lines of studies evidence that HPV may also be an independent risk factor for head and neck cancers. HPV16...
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| Formato: | Artículo revista |
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2021
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/35088 |
| Aporte de: |
| id |
I10-R327-article-35088 |
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ojs |
| institution |
Universidad Nacional de Córdoba |
| institution_str |
I-10 |
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R-327 |
| container_title_str |
Revista de la Facultad de Ciencias Médicas de Córdoba |
| format |
Artículo revista |
| topic |
Human Papillomavirus oral cancer Virus Papiloma Humano cáncer oral |
| spellingShingle |
Human Papillomavirus oral cancer Virus Papiloma Humano cáncer oral MOSMANN , JP KIGUEN , AX VENEZUELA , RF CRISCUOLO , MI TALAVERA , AD LOPEZ DE BLANC , SA CUFFINI , CG HPV 16 mutations associated to oral cancer |
| topic_facet |
Human Papillomavirus oral cancer Virus Papiloma Humano cáncer oral |
| author |
MOSMANN , JP KIGUEN , AX VENEZUELA , RF CRISCUOLO , MI TALAVERA , AD LOPEZ DE BLANC , SA CUFFINI , CG |
| author_facet |
MOSMANN , JP KIGUEN , AX VENEZUELA , RF CRISCUOLO , MI TALAVERA , AD LOPEZ DE BLANC , SA CUFFINI , CG |
| author_sort |
MOSMANN , JP |
| title |
HPV 16 mutations associated to oral cancer |
| title_short |
HPV 16 mutations associated to oral cancer |
| title_full |
HPV 16 mutations associated to oral cancer |
| title_fullStr |
HPV 16 mutations associated to oral cancer |
| title_full_unstemmed |
HPV 16 mutations associated to oral cancer |
| title_sort |
hpv 16 mutations associated to oral cancer |
| description |
Abstract:
Human Papillomavirus (HPV) is the most frequent sexually transmitted infection (STI) worldwide and despite the well-established role of HPV in cervical cancer, new lines of studies evidence that HPV may also be an independent risk factor for head and neck cancers. HPV16 variants and point mutations differ in their ability to establish persistent infections and development of malignancy. HPV16 variants are divided into six lineages: European (EUR), African (AFR 1 and 2), Asian (As), Asian American (AA) and North American (NA).
The aim of this study was to describe the lineages and genetic variability of the E6 and LCR region in the HPV16 sequences obtained from oral cavity lesions samples of patients from Córdoba city.
Oral swabs were taken from different type of oral lesions, identify by specialized stomatology dentist from Faculty of Dentistry, previously. Samples were analysed using PCR method to E6 and LCR HPV16 genes and Sanger sequencing. Genetic sequences and phylogenetic trees were analysed using MEGA6 software.
A total of 23 oral sequences to E6 gene and 8 to LCR gene, were studied. The more frequent point mutations were T350G in 18 sequences and G7521A in 7 sequences, to E6 and LCR genes, respectively. In this study, other point mutations were described too. Concern to HPV16 lineages, all E6 sequences belonged to EUR variant, 7 sequences of LCR were EUR too and 1 of them was AFR2 variant.
This is the first study of lineages and point mutations obtained from oral lesions samples in Argentina. Point mutations associated to malignancies were identify in benign lesions, potentially malignant oral disorders and oral squamous cell carcinomas, highlighted the importance of studying and being able to follow the evolution of the lesion over time, to could offer a correct treatment to the patient. HPV16 infection is a potential risk factor to oral lesions and the knowledge of genetic variations could be an important point to oral cancer prevention. |
| publisher |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2021 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/35088 |
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I10-R327-article-350882024-04-15T16:19:09Z HPV 16 mutations associated to oral cancer Estudio de mutaciones de HPV 16 asociadas a cáncer oral MOSMANN , JP KIGUEN , AX VENEZUELA , RF CRISCUOLO , MI TALAVERA , AD LOPEZ DE BLANC , SA CUFFINI , CG Human Papillomavirus oral cancer Virus Papiloma Humano cáncer oral Abstract: Human Papillomavirus (HPV) is the most frequent sexually transmitted infection (STI) worldwide and despite the well-established role of HPV in cervical cancer, new lines of studies evidence that HPV may also be an independent risk factor for head and neck cancers. HPV16 variants and point mutations differ in their ability to establish persistent infections and development of malignancy. HPV16 variants are divided into six lineages: European (EUR), African (AFR 1 and 2), Asian (As), Asian American (AA) and North American (NA). The aim of this study was to describe the lineages and genetic variability of the E6 and LCR region in the HPV16 sequences obtained from oral cavity lesions samples of patients from Córdoba city. Oral swabs were taken from different type of oral lesions, identify by specialized stomatology dentist from Faculty of Dentistry, previously. Samples were analysed using PCR method to E6 and LCR HPV16 genes and Sanger sequencing. Genetic sequences and phylogenetic trees were analysed using MEGA6 software. A total of 23 oral sequences to E6 gene and 8 to LCR gene, were studied. The more frequent point mutations were T350G in 18 sequences and G7521A in 7 sequences, to E6 and LCR genes, respectively. In this study, other point mutations were described too. Concern to HPV16 lineages, all E6 sequences belonged to EUR variant, 7 sequences of LCR were EUR too and 1 of them was AFR2 variant. This is the first study of lineages and point mutations obtained from oral lesions samples in Argentina. Point mutations associated to malignancies were identify in benign lesions, potentially malignant oral disorders and oral squamous cell carcinomas, highlighted the importance of studying and being able to follow the evolution of the lesion over time, to could offer a correct treatment to the patient. HPV16 infection is a potential risk factor to oral lesions and the knowledge of genetic variations could be an important point to oral cancer prevention. Resumen: El virus del papiloma humano (HPV) es la infección de transmisión sexual más frecuente a nivel mundial y, a pesar del papel bien establecido en el cáncer uterino, nuevas líneas de investigación demuestran que también sería un factor de riesgo independiente para cáncer de cabeza y cuello. Las variantes y mutaciones puntuales del HPV16 difieren en su capacidad para establecer infecciones persistentes y desarrollo de malignidad. Dichas variantes se dividen en seis linajes: europeo(EUR), africano(AFR 1 y 2), asiático(As), asiático americano(AA) y norteamericano(NA). El objetivo fue describir los linajes y la variabilidad genética de las regiones E6 y LCR de HPV16, en muestras de lesiones de la cavidad oral de pacientes de la ciudad de Córdoba. Se tomaron muestras de cepillado bucal de diferentes tipos de lesiones, identificadas previamente por estomatólogos especializados de la Facultad de Odontología. Las muestras se analizaron mediante PCR específica para los genes E6 y LCR del HPV16, seguida de secuenciación por el método de Sanger. El análisis de las secuencias genéticas y la construcción de los árboles filogenéticos se realizaron utilizando el software MEGA6. Estudiamos 23 secuencias orales para el gen E6 y 8 para el gen LCR de HPV16. Las mutaciones puntuales más frecuentes fueron T350G en 18 secuencias y G7521A en 7 secuencias para E6 y LCR, respectivamente; en este estudio se describieron además otras mutaciones puntuales. Con respecto a los linajes de HPV16, todas las secuencias de E6 pertenecieron a la variante EUR; 7 de las 8 secuencias LCR fueron también EUR y solo una secuencia perteneció a la variante AFR2. Este es el primer estudio de linajes y mutaciones de HPV16 en muestras de lesiones bucales en Argentina. Se identificaron mutaciones puntuales asociadas a malignidad en lesiones bucales benignas, desórdenes potencialmente malignos y carcinoma orales; remarcando la importancia de estudiar y seguir la evolución de estas lesiones en los pacientes a lo largo del tiempo para poder brindar un tratamiento adecuado. La infección por HPV16 se considera potecial factor de riesgo para lesiones orales, y el conocimiento de sus variaciones genéticas podría ser importante para la prevención del cáncer oral. Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2021-10-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion texto texto texto https://revistas.unc.edu.ar/index.php/med/article/view/35088 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 78 No. Suplemento (2021): Suplemento JIC XXII Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 78 Núm. Suplemento (2021): Suplemento JIC XXII Revista da Faculdade de Ciências Médicas de Córdoba; v. 78 n. Suplemento (2021): Suplemento JIC XXII 1853-0605 0014-6722 http://creativecommons.org/licenses/by-nc/4.0 |