Characterization of Extracellular Vesicles secreted by fibroblasts associated with breast cancer stimulated with nutritional components of dietary patterns
Evidence indicates a greater adherence to dietary patterns (PD) rich in carbohydrates and saturated fatty acids (represented mainly by fructose (F) and palmitic acid (PA)) with the risk of developing breast cancer. Although cellular mechanisms are not completely elucidated, a possible cause for this...
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| Autores principales: | , , , |
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| Formato: | Artículo revista |
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2022
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/39075 |
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| Sumario: | Evidence indicates a greater adherence to dietary patterns (PD) rich in carbohydrates and saturated fatty acids (represented mainly by fructose (F) and palmitic acid (PA)) with the risk of developing breast cancer. Although cellular mechanisms are not completely elucidated, a possible cause for this association is the intercellular communication mediated by extracellular vesicles (EVs). Our objective was to evaluate in vitro the role of F and AP in the EVs released by cancer-associated fibroblasts (CAFs).
In this sense, CAFs from human mammary adenoma of the F88 line were cultured and stimulated with 40mM F, 250uM AP, F+AP combinations or vehicles for 24h (characteristic concentrations of PD). Supernatants were collected and EVs isolated by sequential ultracentrifugation (2k, 10k, 100k). EVs were observed by transmission electron microscopy for characterization (size and shape) and processed for immunostaining of CD63 EVs marker using colloidal gold, showing a larger number of EVs in F+AP, especially in the 20-30nm particle range; without the treatments altering the EVs shape. In order to evaluate the effect of CAF EVs on cell proliferation, MCF-7 breast epithelial tumor cells were cultured in DMEM 10% FBS and stimulated for 24h with conditioned media and/or with EVs from F88 cells with the different stimuli. MCF7 treated with both conditioned media and EVs from F+AP increased cell proliferation, determined by incorporation of bromodeoxyuridine and cell count (ANOVA, p<0.05).
These results suggest a pathogenic effect of food rich in F and AP on breast cancer tumoral microenvironment, through the release of EVs by CAFs, which induce cell proliferation. |
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