Effectiveness of different desinfections/sterilization protocols for heat sensitive orthodontics instruments

Objective: The aim of this work was to assess effectiveness of different disinfections/ high level disinfection protocols for heat-sensitive orthodontics instruments. Methods: Eight protocols were applied to orthodontic instruments (n=120); they were cleaned with dual- or triple-enzyme detergent and...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autor principal: Zarate et al, Ana María
Formato: Artículo revista
Lenguaje:Español
Publicado: Facultad de Odontología 2017
Materias:
orthodontic
dental instruments
disinfection
sterilization
Acceso en línea:https://revistas.unc.edu.ar/index.php/RevFacOdonto/article/view/16723
Aporte de:
Revista de la Facultad de Odontología de Universidad Nacional de Córdoba
id I10-R335-article-16723
record_format ojs
spelling I10-R335-article-167232017-11-20T03:37:12Z Effectiveness of different desinfections/sterilization protocols for heat sensitive orthodontics instruments Zarate et al, Ana María orthodontic dental instruments disinfection sterilization Objective: The aim of this work was to assess effectiveness of different disinfections/ high level disinfection protocols for heat-sensitive orthodontics instruments. Methods: Eight protocols were applied to orthodontic instruments (n=120); they were cleaned with dual- or triple-enzyme detergent and disinfected with glutaraldehyde 2.5% or hypochlorite 1% or orthophthalaldehyde 0.55%.After applying each protocol, samples were taken from the instruments and cultured in MacConkey Agar and CLED Agar media. For Staphylococcus spp, a coagulase test was used. Instruments were considered contaminated when the CFU count from the cultures was greater than 105 CFU/ml. Results: The same proportion of contamination was found when using glutaraldehyde and sodium hypochlorite, and no significant differences were found with the use of dual-enzyme and triple-enzyme detergents. Of the three disinfectants used, orthophthalaldehyde was significantly more efficient, since contamination was never found when it was used. The microorganisms isolated from the different instruments were Micrococcusspp, Corynebacterium spp, Bacillus sppandcoagulase-negative Staphylococcus. Conclusions: Orthophthalaldehyde was significantly more efficient as a disinfectant. The microorganisms isolated were Micrococcus spp, Corynebacterium spp, Bacillus spp and coagulase-negative Staphylococcus. Although the percentage of contaminated instruments was low in relation to the total monitored, any contaminated instrument implies risks to a patient or a professional of an infectious or contagious disease. Facultad de Odontología 2017-03-21 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/RevFacOdonto/article/view/16723 Revista de la Facultad de Odontología; Vol. 27 Núm. 1 (2017); 19-26 2545-7594 0325-1071 spa https://revistas.unc.edu.ar/index.php/RevFacOdonto/article/view/16723/16480 10.25014/RevFacOdont27166
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-335
container_title_str Revista de la Facultad de Odontología
language Español
format Artículo revista
topic orthodontic
dental instruments
disinfection
sterilization
spellingShingle orthodontic
dental instruments
disinfection
sterilization
Zarate et al, Ana María
Effectiveness of different desinfections/sterilization protocols for heat sensitive orthodontics instruments
topic_facet orthodontic
dental instruments
disinfection
sterilization
author Zarate et al, Ana María
author_facet Zarate et al, Ana María
author_sort Zarate et al, Ana María
title Effectiveness of different desinfections/sterilization protocols for heat sensitive orthodontics instruments
title_short Effectiveness of different desinfections/sterilization protocols for heat sensitive orthodontics instruments
title_full Effectiveness of different desinfections/sterilization protocols for heat sensitive orthodontics instruments
title_fullStr Effectiveness of different desinfections/sterilization protocols for heat sensitive orthodontics instruments
title_full_unstemmed Effectiveness of different desinfections/sterilization protocols for heat sensitive orthodontics instruments
title_sort effectiveness of different desinfections/sterilization protocols for heat sensitive orthodontics instruments
description Objective: The aim of this work was to assess effectiveness of different disinfections/ high level disinfection protocols for heat-sensitive orthodontics instruments. Methods: Eight protocols were applied to orthodontic instruments (n=120); they were cleaned with dual- or triple-enzyme detergent and disinfected with glutaraldehyde 2.5% or hypochlorite 1% or orthophthalaldehyde 0.55%.After applying each protocol, samples were taken from the instruments and cultured in MacConkey Agar and CLED Agar media. For Staphylococcus spp, a coagulase test was used. Instruments were considered contaminated when the CFU count from the cultures was greater than 105 CFU/ml. Results: The same proportion of contamination was found when using glutaraldehyde and sodium hypochlorite, and no significant differences were found with the use of dual-enzyme and triple-enzyme detergents. Of the three disinfectants used, orthophthalaldehyde was significantly more efficient, since contamination was never found when it was used. The microorganisms isolated from the different instruments were Micrococcusspp, Corynebacterium spp, Bacillus sppandcoagulase-negative Staphylococcus. Conclusions: Orthophthalaldehyde was significantly more efficient as a disinfectant. The microorganisms isolated were Micrococcus spp, Corynebacterium spp, Bacillus spp and coagulase-negative Staphylococcus. Although the percentage of contaminated instruments was low in relation to the total monitored, any contaminated instrument implies risks to a patient or a professional of an infectious or contagious disease.
publisher Facultad de Odontología
publishDate 2017
url https://revistas.unc.edu.ar/index.php/RevFacOdonto/article/view/16723
work_keys_str_mv AT zarateetalanamaria effectivenessofdifferentdesinfectionssterilizationprotocolsforheatsensitiveorthodonticsinstruments
first_indexed 2024-09-03T21:14:53Z
last_indexed 2024-09-03T21:14:53Z
_version_ 1809211031045537792

Ejemplares similares