Determination of glassy state by cryo-SEM and DSC in cryopreservation of mint shoot tips by encapsulation–dehydration
Cryopreservation of mint shoot tips grown in vitro (Mentha 9 piperita) was performed after encap- sulation in alginate beads. Encapsulated shoot tips were first precultured in sucrose enriched medium (0.75 M) and then dried under a sterile air flow (0-6 h). After cooling in liquid nitrogen and warmi...
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| Autores principales: | , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2014
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/145975 |
| Aporte de: |
| Sumario: | Cryopreservation of mint shoot tips grown in vitro (Mentha 9 piperita) was performed after encap- sulation in alginate beads. Encapsulated shoot tips were first precultured in sucrose enriched medium (0.75 M) and then dried under a sterile air flow (0-6 h). After cooling in liquid nitrogen and warming in a warm water bath, alginate beads were transferred to solid culture medium for 4 weeks. The effect of dehydration time of the encapsu- lated shoots was evaluated for water content, cooling and warming rates, ice crystal formation and cellular vitrifica- tion, by using low temperature scanning electron micros- copy and differential scanning calorimetry. Viability of the recovered material showed a close relation between the dehydration time, cooling and warming rates, ice formation avoidance and tissue vitrification. At short drying periods (up to 3 h), ice crystals were formed and the viability was low or absent. After longer drying periods (5 and 6 h), both beads and specimens became vitrified. |
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