Determination of folic acid and its related substances of the ferrous fumarate and folic acid dispersible tablet by HPLC
The aim of the present study was to establish a method for the determination of folic acid and related substances content in dispersible tablets. Method: A Shim-pack VP-ODS C18 reversed phase column (4.6 mm × 250 mm) was used. The mobile phase consisted of methanol-phosphate buffer (20:80) with a pH...
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| Autores principales: | , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2012
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/20182 http://www.latamjpharm.org/resumenes/31/4/LAJOP_31_4_1_10.pdf |
| Aporte de: |
| Sumario: | The aim of the present study was to establish a method for the determination of folic acid and related substances content in dispersible tablets. Method: A Shim-pack VP-ODS C18 reversed phase column (4.6 mm × 250 mm) was used. The mobile phase consisted of methanol-phosphate buffer (20:80) with a pH of 6.3.The flow rate was 1.0 mL/min, and the detection wavelength was 277nm and the column temperature was 30 °C. Results: The calibration curve was linear in the range of 5~150 μg/mL (r = 0.9998) for folic acid. The minimal detection limit was 99.08 %, n = 9 and the related substances were well separated.
This method resulted to be convenient, accurate, selective and reliable, and can be applied for the quality control of folic acid. |
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