Interaction of high density lipoprotein particles with membranes containing cholesterol

In this study, free cholesterol (FC) efflux mediated by human HDL was investigated using fluorescence methodologies. The accessibility of FC to HDL may depend on whether it is located in regions rich in unsaturated phospholipids or in domains containing high levels of FC and sphingomyelin, known as...

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Detalles Bibliográficos
Autores principales: Sanchez, Susana A., Tricerri, María Alejandra, Gratton, Enrico
Formato: Articulo
Lenguaje:Inglés
Publicado: 2007
Materias:
Bioquímica
6-lauroyl-2-(dimethylamino) naphthalene
Giant unilamellar vesicles
Raft mixtures
Two-photon microscopy
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/82978
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-82978
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Bioquímica
6-lauroyl-2-(dimethylamino) naphthalene
Giant unilamellar vesicles
Raft mixtures
Two-photon microscopy
spellingShingle Bioquímica
6-lauroyl-2-(dimethylamino) naphthalene
Giant unilamellar vesicles
Raft mixtures
Two-photon microscopy
Sanchez, Susana A.
Tricerri, María Alejandra
Gratton, Enrico
Interaction of high density lipoprotein particles with membranes containing cholesterol
topic_facet Bioquímica
6-lauroyl-2-(dimethylamino) naphthalene
Giant unilamellar vesicles
Raft mixtures
Two-photon microscopy
description In this study, free cholesterol (FC) efflux mediated by human HDL was investigated using fluorescence methodologies. The accessibility of FC to HDL may depend on whether it is located in regions rich in unsaturated phospholipids or in domains containing high levels of FC and sphingomyelin, known as "lipid rafts." Laurdan generalized polarization and two-photon microscopy were used to quantify FC removal from different pools in the bilayer of giant unilamellar vesicles (GUVs). GUVs made of POPC and FC were observed after incubation with reconstituted particles containing apolipoprotein A-I and POPC [78Å diameter reconstituted high density lipoprotein (rHDL)]. Fluorescence correlation spectroscopy data show an increase in rHDL size during the incubation period. GUVs made of two "raft-like" mixtures [DOPC/DPPC/FC (1:1:1) and POPC/SPM/FC (6:1:1)] were used to model liquid-ordered/liquid-disordered phase coexistence. Through these experiments, we conclude that rHDL preferentially removes cholesterol from the more fluid phases. These data, and their extrapolation to in vivo systems, show the significant role that phase separation plays in the regulation of cholesterol homeostasis.
format Articulo
Articulo
author Sanchez, Susana A.
Tricerri, María Alejandra
Gratton, Enrico
author_facet Sanchez, Susana A.
Tricerri, María Alejandra
Gratton, Enrico
author_sort Sanchez, Susana A.
title Interaction of high density lipoprotein particles with membranes containing cholesterol
title_short Interaction of high density lipoprotein particles with membranes containing cholesterol
title_full Interaction of high density lipoprotein particles with membranes containing cholesterol
title_fullStr Interaction of high density lipoprotein particles with membranes containing cholesterol
title_full_unstemmed Interaction of high density lipoprotein particles with membranes containing cholesterol
title_sort interaction of high density lipoprotein particles with membranes containing cholesterol
publishDate 2007
url http://sedici.unlp.edu.ar/handle/10915/82978
work_keys_str_mv AT sanchezsusanaa interactionofhighdensitylipoproteinparticleswithmembranescontainingcholesterol
AT tricerrimariaalejandra interactionofhighdensitylipoproteinparticleswithmembranescontainingcholesterol
AT grattonenrico interactionofhighdensitylipoproteinparticleswithmembranescontainingcholesterol
bdutipo_str Repositorios
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