Isolation of equine herpesvirus-2 from the lung of an aborted fetus

This study describes the isolation of equine herpesvirus-2 (EHV-2) from the lung of an aborted equine fetus in Argentina. The isolated virus was confirmed as EHV-2 by indirect immunofluorescence using a rabbit anti-EHV-2 polyclonal antiserum and by virus-neutralization test using an equine polyclona...

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Detalles Bibliográficos
Autores principales: Galosi, Cecilia Mónica, Cid de La Paz, Viviana, Fernández, Leandro Carlos, Martínez, Javier Pablo, Craig, María Isabel, Barrandeguy, María, Etcheverrigaray, María Elisa
Formato: Articulo Comunicacion
Lenguaje:Inglés
Publicado: 2005
Materias:
Ciencias Veterinarias
Aborted fetus
Equine herpesvirus-2
Isolation
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/83372
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
Descripción
Sumario:This study describes the isolation of equine herpesvirus-2 (EHV-2) from the lung of an aborted equine fetus in Argentina. The isolated virus was confirmed as EHV-2 by indirect immunofluorescence using a rabbit anti-EHV-2 polyclonal antiserum and by virus-neutralization test using an equine polyclonal antibody against EHV-2. Restriction endonuclease DNA fingerprinting with BamHI also confirmed the identity of the virus as EHV-2. Furthermore, viral nucleic acid was detected by polymerase chain reaction from the original lung sample and from the DNA obtained from cells infected with the virus isolate. This work constitutes the first reported isolation of EHV-2 from an aborted equine fetus. The presence of EHV-2 in the lung of the aborted fetus would indicate that this virus is capable of crossing the placental barrier. However, no cause-effect relationship was established between the EHV-2 isolate and the abortion.

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