Mitochondrial variability in the D-loop of four equine breeds shown by PCR-SSCP analysis

A fragment of 466 base pairs from a highly variable peripheral region of the mitochondrial D-loop of horses was amplified and analyzed by single stranded conformational polymorphism (SSCP). Fourteen distinct SSCP variants were detected in 100 horses belonging to four breeds (Arabian, ARB; Thoroughbr...

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Detalles Bibliográficos
Autores principales: Mirol, Patricia Mónica, Peral García, Pilar, Dulout, Fernando Noel
Formato: Articulo
Lenguaje:Inglés
Publicado: 2002
Materias:
Ciencias Veterinarias
Equine
MTDNA
PCR
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/83578
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-83578
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Equine
MTDNA
PCR
spellingShingle Ciencias Veterinarias
Equine
MTDNA
PCR
Mirol, Patricia Mónica
Peral García, Pilar
Dulout, Fernando Noel
Mitochondrial variability in the D-loop of four equine breeds shown by PCR-SSCP analysis
topic_facet Ciencias Veterinarias
Equine
MTDNA
PCR
description A fragment of 466 base pairs from a highly variable peripheral region of the mitochondrial D-loop of horses was amplified and analyzed by single stranded conformational polymorphism (SSCP). Fourteen distinct SSCP variants were detected in 100 horses belonging to four breeds (Arabian, ARB; Thoroughbred, TB; Argentinian Creole, ARC; and Peruvian Paso from Argentina, PPA). Each breed showed four to eight SSCP variants, many of which were shared between two or three of the studied breeds. Arabian horses were the most variable (eight variants), with three variants unique to the breed. PPA and ARC showed two and one characteristic SSCP variants, respectively, while TB shared all its variants with at least one of the other breeds. An analysis based on the presence/absence of the variants revealed a closer relationship between PPA and TB, which was not completely unexpected considering the mixed ancestry of the PPA mares. The results also confirm the efficiency of SSCP to detect variability in horse mitochondrial DNA.
format Articulo
Articulo
author Mirol, Patricia Mónica
Peral García, Pilar
Dulout, Fernando Noel
author_facet Mirol, Patricia Mónica
Peral García, Pilar
Dulout, Fernando Noel
author_sort Mirol, Patricia Mónica
title Mitochondrial variability in the D-loop of four equine breeds shown by PCR-SSCP analysis
title_short Mitochondrial variability in the D-loop of four equine breeds shown by PCR-SSCP analysis
title_full Mitochondrial variability in the D-loop of four equine breeds shown by PCR-SSCP analysis
title_fullStr Mitochondrial variability in the D-loop of four equine breeds shown by PCR-SSCP analysis
title_full_unstemmed Mitochondrial variability in the D-loop of four equine breeds shown by PCR-SSCP analysis
title_sort mitochondrial variability in the d-loop of four equine breeds shown by pcr-sscp analysis
publishDate 2002
url http://sedici.unlp.edu.ar/handle/10915/83578
work_keys_str_mv AT mirolpatriciamonica mitochondrialvariabilityinthedloopoffourequinebreedsshownbypcrsscpanalysis
AT peralgarciapilar mitochondrialvariabilityinthedloopoffourequinebreedsshownbypcrsscpanalysis
AT duloutfernandonoel mitochondrialvariabilityinthedloopoffourequinebreedsshownbypcrsscpanalysis
bdutipo_str Repositorios
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