Methyl-β-cyclodextrins preferentially remove cholesterol from the liquid disordered phase in giant unilamellar vesicles

Methyl-β-cyclodextrins (MβCDs) are molecules that are extensively used to remove and to load cholesterol (Chol) from artificial and natural membranes; however, the mechanism of Chol extraction by MβCD from pure lipids or from complex mixtures is not fully understood. One of the outstanding questions...

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Detalles Bibliográficos
Autores principales: Sánchez, Susana A., Gunther, German, Tricerri, María Alejandra, Gratton, Enrico
Formato: Articulo
Lenguaje:Inglés
Publicado: 2011
Materias:
Ciencias Médicas
Cholesterol
GUV
Lipid mixture
MβCD
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/83977
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-83977
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Cholesterol
GUV
Lipid mixture
MβCD
spellingShingle Ciencias Médicas
Cholesterol
GUV
Lipid mixture
MβCD
Sánchez, Susana A.
Gunther, German
Tricerri, María Alejandra
Gratton, Enrico
Methyl-β-cyclodextrins preferentially remove cholesterol from the liquid disordered phase in giant unilamellar vesicles
topic_facet Ciencias Médicas
Cholesterol
GUV
Lipid mixture
MβCD
description Methyl-β-cyclodextrins (MβCDs) are molecules that are extensively used to remove and to load cholesterol (Chol) from artificial and natural membranes; however, the mechanism of Chol extraction by MβCD from pure lipids or from complex mixtures is not fully understood. One of the outstanding questions in this field is the capability of MβCD to remove Chol from lipid domains having different packing. Here, we investigated the specificity of MβCD to remove Chol from coexisting macrodomains with different lipid packing. We used giant unilamellar vesicles (GUVs) made of 1,2- dioleoylphosphatidylcholine:1,2-dipalmitoylphatidylcholine:free cholesterol, 1:1:1 molar ratio at 27°C. Under these conditions, individual GUVs present Chol distributed into l o and l d phases. The two phases can be distinguished and visualized using Laurdan generalized polarization and two-photon excitation fluorescence microscopy. Our data indicate that MβCD removes Chol preferentially from the more disordered phase. The process of selective Chol removal is dependent on the MβCD concentration. At high concentrations, MβCD also removes phospholipids.
format Articulo
Articulo
author Sánchez, Susana A.
Gunther, German
Tricerri, María Alejandra
Gratton, Enrico
author_facet Sánchez, Susana A.
Gunther, German
Tricerri, María Alejandra
Gratton, Enrico
author_sort Sánchez, Susana A.
title Methyl-β-cyclodextrins preferentially remove cholesterol from the liquid disordered phase in giant unilamellar vesicles
title_short Methyl-β-cyclodextrins preferentially remove cholesterol from the liquid disordered phase in giant unilamellar vesicles
title_full Methyl-β-cyclodextrins preferentially remove cholesterol from the liquid disordered phase in giant unilamellar vesicles
title_fullStr Methyl-β-cyclodextrins preferentially remove cholesterol from the liquid disordered phase in giant unilamellar vesicles
title_full_unstemmed Methyl-β-cyclodextrins preferentially remove cholesterol from the liquid disordered phase in giant unilamellar vesicles
title_sort methyl-β-cyclodextrins preferentially remove cholesterol from the liquid disordered phase in giant unilamellar vesicles
publishDate 2011
url http://sedici.unlp.edu.ar/handle/10915/83977
work_keys_str_mv AT sanchezsusanaa methylbcyclodextrinspreferentiallyremovecholesterolfromtheliquiddisorderedphaseingiantunilamellarvesicles
AT gunthergerman methylbcyclodextrinspreferentiallyremovecholesterolfromtheliquiddisorderedphaseingiantunilamellarvesicles
AT tricerrimariaalejandra methylbcyclodextrinspreferentiallyremovecholesterolfromtheliquiddisorderedphaseingiantunilamellarvesicles
AT grattonenrico methylbcyclodextrinspreferentiallyremovecholesterolfromtheliquiddisorderedphaseingiantunilamellarvesicles
bdutipo_str Repositorios
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