Membrane-bound glycerol dehydrogenase catalyzes oxidation of D-pentonates to 4-keto-D-pentonates, D-fructose to 5-keto-D-fructose, and D-psicose to 5-keto-D-psicose

A novel oxidation of D-pentonates to 4-keto-D-pentonates was analyzed with <i>Gluconobacter Thailandicus</i> NBRC 3258. D-Pentonate 4-dehydrogenase activity in the membrane fraction was readily inactivated by EDTA and it was reactivated by the addition of PQQ and Ca<sup>2+</sup&...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Ano, Yoshitaka, Hours, Roque Alberto, Akakabe, Yoshihiko, Kataoka, Naoya, Yakushi, Toshiharu, Matsushita, Kazunobu, Adachi, Osao
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Ciencias Exactas
4-keto-D-arabonate production
4-keto-D-pentonate
Acetic acid bacteria
Glycerol dehydrogenase
Oxidative fermentation
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/87580
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
Descripción
Sumario:A novel oxidation of D-pentonates to 4-keto-D-pentonates was analyzed with <i>Gluconobacter Thailandicus</i> NBRC 3258. D-Pentonate 4-dehydrogenase activity in the membrane fraction was readily inactivated by EDTA and it was reactivated by the addition of PQQ and Ca<sup>2+</sup>. D-Pentonate 4-dehydrogenase was purified to two different subunits, 80 and 14 kDa. The absorption spectrum of the purified enzyme showed no typical absorbance over the visible regions. The enzyme oxidized D-pentonates to 4-keto-D-pentonates at the optimum pH of 4.0. In addition, the enzyme oxidized D-fructose to 5-keto-D-fructose, D-psicose to 5-keto-D-psicose, including the other polyols such as, glycerol, D-ribitol, D-arabitol, and D-sorbitol. Thus, D-pentonate 4-dehydrogenase was found to be identical with glycerol dehydrogenase (GLDH), a major polyol dehydrogenase in <i>Gluconobacter</i> species. The reaction versatility of quinoprotein GLDH was notified in this study.

Ejemplares similares