Papain purification insights: monitoring by electrophoretic approaches and MALDI-TOF peptide mass fingerprint analyses

Papain was purified from dried Carica papaya latex by fractioned salt precipitation in presence of sodium tetrathionate to preserve enzymatic activity. Purification was followed by different electrophoretic methods. Identification of the purified product was afforded by submitting the peptides obtai...

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Detalles Bibliográficos
Autores principales: Llerena Suster, Carlos Rafael, Obregón, Walter David, Trejo, Sebastián Alejandro, Morcelle del Valle, Susana Raquel
Formato: Articulo
Lenguaje:Inglés
Publicado: 2011
Materias:
Biología
Caricain
Chymopapain
Glycyl endopeptidase
MALDI-TOF
Peptide mass fingerprint
Papain
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/92371
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata

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