Equine arteritis virus gP5 protein induces apoptosis in cultured insect cells

Equine Arteritis Virus (EAV) has been shown to induce apoptosis in vitro but the induction of this mechanism has not been previously associated with any viral gene product. In this work, we found a cytotoxicity effect of the EAV gP5 protein on baculovirus-insect cells and a low yield of protein reco...

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Detalles Bibliográficos
Autores principales: Metz, Germán Ernesto, Serena, María Soledad, Abeyá, María Mercedes, Dulbecco, Andrea Belén, Massone, Adriana Raquel, Díaz, Silvina, Echeverría, María Gabriela
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2014
Materias:
Ciencias Veterinarias
Equine arteritis virus
Apoptosis
gP5 protein
Insect cells
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/98898
https://ri.conicet.gov.ar/11336/11504
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
Descripción
Sumario:Equine Arteritis Virus (EAV) has been shown to induce apoptosis in vitro but the induction of this mechanism has not been previously associated with any viral gene product. In this work, we found a cytotoxicity effect of the EAV gP5 protein on baculovirus-insect cells and a low yield of protein recovery. Besides, different morphological features by electron transmission microscopy, DNA fragmentation in agarose gel, TUNEL analysis and caspase 3 activity were found. All these findings indicate that the EAV gP5 protein induces apoptosis in insect cells.

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