Fruit Rot Caused by Phytophthora sp. in Cold-Stored Pears in the Valley of Rio Negro and Neuquén, Argentina

Pear fruit conservation for long periods is associated with postharvest fungal diseases. In Argentina, the most important and widespread diseases are caused by Penicillium spp. and Botrytis cinerea, followed by Alternaria sp. and Cladosporium sp. and Athelia epiphylla. The last one can be important...

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Detalles Bibliográficos
Autores principales: Dobra, Alicia Cristina, Sosa, María Cristina, Lutz, María Cecilia, Rodriguez, Gustavo, Greslebin, Alina G., Vélez, A. G
Formato: Articulo article acceptedVersion
Lenguaje:Inglés
Publicado: ISHS 2011
Materias:
Phytophthora
Postharvest diseases
Fruit rot
Williams
Packham’s Triumph
Pears
https://purl.org/becyt/ford/4.1
Ciencias Agrarias y Forestales
Acceso en línea:http://rdi.uncoma.edu.ar/handle/uncomaid/15547
Aporte de:
Repositorio Institucional UNCo de Universidad Nacional del Comahue
id I22-R178-uncomaid-15547
record_format dspace
institution Universidad Nacional del Comahue
institution_str I-22
repository_str R-178
collection Repositorio Institucional UNCo
language Inglés
topic Phytophthora
Postharvest diseases
Fruit rot
Williams
Packham’s Triumph
Pears
https://purl.org/becyt/ford/4.1
Ciencias Agrarias y Forestales
spellingShingle Phytophthora
Postharvest diseases
Fruit rot
Williams
Packham’s Triumph
Pears
https://purl.org/becyt/ford/4.1
Ciencias Agrarias y Forestales
Dobra, Alicia Cristina
Sosa, María Cristina
Lutz, María Cecilia
Rodriguez, Gustavo
Greslebin, Alina G.
Vélez, A. G
Fruit Rot Caused by Phytophthora sp. in Cold-Stored Pears in the Valley of Rio Negro and Neuquén, Argentina
topic_facet Phytophthora
Postharvest diseases
Fruit rot
Williams
Packham’s Triumph
Pears
https://purl.org/becyt/ford/4.1
Ciencias Agrarias y Forestales
description Pear fruit conservation for long periods is associated with postharvest fungal diseases. In Argentina, the most important and widespread diseases are caused by Penicillium spp. and Botrytis cinerea, followed by Alternaria sp. and Cladosporium sp. and Athelia epiphylla. The last one can be important depending on the fruit-lot. This study reports the presence of one Oomycete, as the cause of pear fruit rot in postharvest. In 2010, during the first months of conservation of ‘Williams’, ‘Packham’s Triumph’ and ‘Red Bartlet’ pears, in cold storage, in the eastern area of Alto Valle of Río Negro, fruit decay was recorded with losses between 5 and 20%, according to fruit-lot. Symptoms of decay were studied and its ethiology was determined using conventional and molecular methods. Fruit started showing circular, light to dark brown areas with irregular and diffuse margins that spread rapidly. The decayed area remained firm. The lesion diameter increased and it extended to pulp tissue to reach fruit core with a hyperbolic shape, different from the spherical shape caused by Penicillium spp. or Botrytis sp. The pathogen was isolated in V8 agar selective medium from pears with symptoms. By morphologic characteristics (colony and sporangia) of isolates, its association with clade 6 of Phytophthora was determined. The identification of isolates was confirmed by direct sequencing of the ITS rDNA region using DC6 and ITS4 primers. The nucleotide sequence showed 100% of similarity (745/745 pb) with sequences available in GenBank and was identified as an undescribed species inside P. gonapodyides- P. megasperma Clade 6. This is the first report of a new Phytophthora in postharvest pears.
format Articulo
article
acceptedVersion
author Dobra, Alicia Cristina
Sosa, María Cristina
Lutz, María Cecilia
Rodriguez, Gustavo
Greslebin, Alina G.
Vélez, A. G
author_facet Dobra, Alicia Cristina
Sosa, María Cristina
Lutz, María Cecilia
Rodriguez, Gustavo
Greslebin, Alina G.
Vélez, A. G
author_sort Dobra, Alicia Cristina
title Fruit Rot Caused by Phytophthora sp. in Cold-Stored Pears in the Valley of Rio Negro and Neuquén, Argentina
title_short Fruit Rot Caused by Phytophthora sp. in Cold-Stored Pears in the Valley of Rio Negro and Neuquén, Argentina
title_full Fruit Rot Caused by Phytophthora sp. in Cold-Stored Pears in the Valley of Rio Negro and Neuquén, Argentina
title_fullStr Fruit Rot Caused by Phytophthora sp. in Cold-Stored Pears in the Valley of Rio Negro and Neuquén, Argentina
title_full_unstemmed Fruit Rot Caused by Phytophthora sp. in Cold-Stored Pears in the Valley of Rio Negro and Neuquén, Argentina
title_sort fruit rot caused by phytophthora sp. in cold-stored pears in the valley of rio negro and neuquén, argentina
publisher ISHS
publishDate 2011
url http://rdi.uncoma.edu.ar/handle/uncomaid/15547
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spelling I22-R178-uncomaid-155472024-04-26T14:29:25Z Fruit Rot Caused by Phytophthora sp. in Cold-Stored Pears in the Valley of Rio Negro and Neuquén, Argentina Dobra, Alicia Cristina Sosa, María Cristina Lutz, María Cecilia Rodriguez, Gustavo Greslebin, Alina G. Vélez, A. G Phytophthora Postharvest diseases Fruit rot Williams Packham’s Triumph Pears https://purl.org/becyt/ford/4.1 Ciencias Agrarias y Forestales Pear fruit conservation for long periods is associated with postharvest fungal diseases. In Argentina, the most important and widespread diseases are caused by Penicillium spp. and Botrytis cinerea, followed by Alternaria sp. and Cladosporium sp. and Athelia epiphylla. The last one can be important depending on the fruit-lot. This study reports the presence of one Oomycete, as the cause of pear fruit rot in postharvest. In 2010, during the first months of conservation of ‘Williams’, ‘Packham’s Triumph’ and ‘Red Bartlet’ pears, in cold storage, in the eastern area of Alto Valle of Río Negro, fruit decay was recorded with losses between 5 and 20%, according to fruit-lot. Symptoms of decay were studied and its ethiology was determined using conventional and molecular methods. Fruit started showing circular, light to dark brown areas with irregular and diffuse margins that spread rapidly. The decayed area remained firm. The lesion diameter increased and it extended to pulp tissue to reach fruit core with a hyperbolic shape, different from the spherical shape caused by Penicillium spp. or Botrytis sp. The pathogen was isolated in V8 agar selective medium from pears with symptoms. By morphologic characteristics (colony and sporangia) of isolates, its association with clade 6 of Phytophthora was determined. The identification of isolates was confirmed by direct sequencing of the ITS rDNA region using DC6 and ITS4 primers. The nucleotide sequence showed 100% of similarity (745/745 pb) with sequences available in GenBank and was identified as an undescribed species inside P. gonapodyides- P. megasperma Clade 6. This is the first report of a new Phytophthora in postharvest pears. La conservación del fruto de pera por períodos prolongados se asocia con enfermedades fúngicas poscosecha. En Argentina las enfermedades más importantes y extendidas son causadas por Penicillium spp. y Botrytis cinerea , seguida de Alternaria sp. y Cladosporium sp. y Athelia epiphylla . Este último puede ser importante dependiendo del lote de fruta. Este estudio reporta la presencia de un Oomiceto, como causante de la pudrición del fruto de pera en poscosecha. En 2010, durante los primeros meses de conservación de peras 'Williams', 'Packham's Triumph' y 'Red Bartlet', en cámaras frigoríficas, en la zona oriental del Alto Valle de Río Negro, se registró pudrición de frutos con pérdidas entre 5 y 20 %, según lote de fruto. Se estudiaron los síntomas de la descomposición y se determinó su etiología mediante métodos convencionales y moleculares. Los frutos comenzaron a mostrar áreas circulares, de color marrón claro a oscuro, con márgenes irregulares y difusos que se extendieron rápidamente. La zona deteriorada se mantuvo firme. El diámetro de la lesión aumentó y se extendió al tejido pulpar hasta llegar al corazón del fruto con forma hiperbólica, diferente a la forma esférica causada por Penicillium spp. o Botrytis sp. El patógeno se aisló en medio selectivo agar V8 de peras con síntomas. Por características morfológicas (colonia y esporangios) de los aislados se determinó su asociación con el clado 6 de Phytophthora . La identificación de los aislados se confirmó mediante secuenciación directa de la región ITS rDNA utilizando cebadores DC6 e ITS4. La secuencia de nucleótidos mostró 100% de similitud (745/745 pb) con secuencias disponibles en GenBank y fue identificada como una especie no descrita dentro de P. gonapodyides - P. megasperma Clado 6. Este es el primer reporte de una nueva Phytophthora en peras poscosecha. Fil: Dobra, Alicia Cristina. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina. Fil: Sosa, María Cristina. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina. Fil: Lutz, María Cecilia. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina. Fil: Rodriguez, Gustavo. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina. 2011 2019-12-03T00:02:02Z 2019-12-03T00:02:02Z Articulo article acceptedVersion http://rdi.uncoma.edu.ar/handle/uncomaid/15547 0567-7572 eng https://www.ishs.org/ishs-article/909_59 Atribución-NoComercial-CompartirIgual 2.5 Argentina https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ application/pdf pp. 505-510 application/pdf ISHS Acta Horticulturae. XI International Pear Symposium. Acta Horticulturae 909

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