Hypothesis-driven dragging of transcriptomic data to analyze proven targeted pathways in Rhinella arenarum larvae exposed to organophosphorus pesticides

Transcriptional analysis of the network of transcription regulators and target pathways in exposed organisms may be a hard task when their genome remains unknown. The development of hundreds of qPCR assays, including primer design and normalization of the results with the appropriate housekeeping ge...

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Autores principales: Pires, Natalia S., Lascano, Cecilia, Ousset, Julia, Ceschin, Danilo G., Venturino, Andrés
Formato: Articulo article acceptedVersion
Lenguaje:Inglés
Publicado: Nature Publishing Group 2022
Materias:
Toxicogenomic
Rhinella arenarum
Biología molecular
Ciencias de la Tierra y Medio Ambiente
Ciencias Agrarias y Forestales
Acceso en línea:http://rdi.uncoma.edu.ar/handle/uncomaid/17808
Aporte de:
Repositorio Institucional UNCo de Universidad Nacional del Comahue
id I22-R178-uncomaid-17808
record_format dspace
spelling I22-R178-uncomaid-178082024-04-08T00:39:45Z Hypothesis-driven dragging of transcriptomic data to analyze proven targeted pathways in Rhinella arenarum larvae exposed to organophosphorus pesticides Pires, Natalia S. Lascano, Cecilia Ousset, Julia Ceschin, Danilo G. Venturino, Andrés Toxicogenomic Rhinella arenarum Biología molecular Ciencias de la Tierra y Medio Ambiente Ciencias Agrarias y Forestales Transcriptional analysis of the network of transcription regulators and target pathways in exposed organisms may be a hard task when their genome remains unknown. The development of hundreds of qPCR assays, including primer design and normalization of the results with the appropriate housekeeping genes, seems an unreachable task. Alternatively, we took advantage of a whole transcriptome study on Rhinella arenarum larvae exposed to the organophosphorus pesticides azinphos-methyl and chlorpyrifos to evaluate the transcriptional effects on a priori selected groups of genes. This approach allowed us to evaluate the effects on hypothesis-selected pathways such as target esterases, detoxifying enzymes, polyamine metabolism and signaling, and regulatory pathways modulating them. We could then compare the responses at the transcriptional level with previously described effects at the enzymatic or metabolic levels to obtain global insight into toxicity–response mechanisms. The effects of both pesticides on the transcript levels of these pathways could be considered moderate, while chlorpyrifos-induced responses were more potent and earlier than those elicited by azinphos-methyl. Finally, we inferred a prevailing downregulation effect of pesticides on signaling pathways and transcription factor transcripts encoding products that modulate/control the polyamine and antioxidant response pathways. We also tested and selected potential housekeeping genes based on those reported for other species. These results allow us to conduct future confirmatory studies on pesticide modulation of gene expression in toad larvae. Fil: Pires, Natalia S. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina. Fil: Pires, Natalia S. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina. Fil: Lascano, Cecilia. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina. Fil: Lascano, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina. Fil: Ousset, Julia. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina. Fil: Ousset, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina. Fil: Ceschin, Danilo G. Centro de Investigación en Medicina Traslacional “Severo R. Amuchástegui; Argentina. Fil: Ceschin, Danilo G. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra ; Argentina. Fil: Venturino, Andrés. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina Fil: Venturino, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina. 2022 2024-04-04T17:44:14Z 2024-04-04T17:44:14Z Articulo article acceptedVersion 2045-2322 http://rdi.uncoma.edu.ar/handle/uncomaid/17808 eng https://doi.org/10.1038/s41598-022-21748-6 Atribución-NoComercial-CompartirIgual 2.5 Argentina https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ application/pdf application/pdf Nature Publishing Group Scientific Reports 12:17712
institution Universidad Nacional del Comahue
institution_str I-22
repository_str R-178
collection Repositorio Institucional UNCo
language Inglés
topic Toxicogenomic
Rhinella arenarum
Biología molecular
Ciencias de la Tierra y Medio Ambiente
Ciencias Agrarias y Forestales
spellingShingle Toxicogenomic
Rhinella arenarum
Biología molecular
Ciencias de la Tierra y Medio Ambiente
Ciencias Agrarias y Forestales
Pires, Natalia S.
Lascano, Cecilia
Ousset, Julia
Ceschin, Danilo G.
Venturino, Andrés
Hypothesis-driven dragging of transcriptomic data to analyze proven targeted pathways in Rhinella arenarum larvae exposed to organophosphorus pesticides
topic_facet Toxicogenomic
Rhinella arenarum
Biología molecular
Ciencias de la Tierra y Medio Ambiente
Ciencias Agrarias y Forestales
description Transcriptional analysis of the network of transcription regulators and target pathways in exposed organisms may be a hard task when their genome remains unknown. The development of hundreds of qPCR assays, including primer design and normalization of the results with the appropriate housekeeping genes, seems an unreachable task. Alternatively, we took advantage of a whole transcriptome study on Rhinella arenarum larvae exposed to the organophosphorus pesticides azinphos-methyl and chlorpyrifos to evaluate the transcriptional effects on a priori selected groups of genes. This approach allowed us to evaluate the effects on hypothesis-selected pathways such as target esterases, detoxifying enzymes, polyamine metabolism and signaling, and regulatory pathways modulating them. We could then compare the responses at the transcriptional level with previously described effects at the enzymatic or metabolic levels to obtain global insight into toxicity–response mechanisms. The effects of both pesticides on the transcript levels of these pathways could be considered moderate, while chlorpyrifos-induced responses were more potent and earlier than those elicited by azinphos-methyl. Finally, we inferred a prevailing downregulation effect of pesticides on signaling pathways and transcription factor transcripts encoding products that modulate/control the polyamine and antioxidant response pathways. We also tested and selected potential housekeeping genes based on those reported for other species. These results allow us to conduct future confirmatory studies on pesticide modulation of gene expression in toad larvae.
format Articulo
article
acceptedVersion
author Pires, Natalia S.
Lascano, Cecilia
Ousset, Julia
Ceschin, Danilo G.
Venturino, Andrés
author_facet Pires, Natalia S.
Lascano, Cecilia
Ousset, Julia
Ceschin, Danilo G.
Venturino, Andrés
author_sort Pires, Natalia S.
title Hypothesis-driven dragging of transcriptomic data to analyze proven targeted pathways in Rhinella arenarum larvae exposed to organophosphorus pesticides
title_short Hypothesis-driven dragging of transcriptomic data to analyze proven targeted pathways in Rhinella arenarum larvae exposed to organophosphorus pesticides
title_full Hypothesis-driven dragging of transcriptomic data to analyze proven targeted pathways in Rhinella arenarum larvae exposed to organophosphorus pesticides
title_fullStr Hypothesis-driven dragging of transcriptomic data to analyze proven targeted pathways in Rhinella arenarum larvae exposed to organophosphorus pesticides
title_full_unstemmed Hypothesis-driven dragging of transcriptomic data to analyze proven targeted pathways in Rhinella arenarum larvae exposed to organophosphorus pesticides
title_sort hypothesis-driven dragging of transcriptomic data to analyze proven targeted pathways in rhinella arenarum larvae exposed to organophosphorus pesticides
publisher Nature Publishing Group
publishDate 2022
url http://rdi.uncoma.edu.ar/handle/uncomaid/17808
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