Identificación de epitopes de Trypanosoma cruzi mediante análisis in vitro de la respuesta celular en pacientes con enfermedad de Chagas crónica
Cellular immunity, and T cell-mediated response in particular, plays a fundamental role in controlling Trypanosoma cruzi infection. In chronically infected patients, it has been shown to be involved in progression from the asymptomatic phase of the disease to chronic Chagas cardiopathy. However, the...
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| Formato: | Tesis doctoral acceptedVersion |
| Lenguaje: | Español |
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Facultad de Farmacia y Bioquímica
2018
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_2674 http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_2674.dir/2674.PDF |
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| Sumario: | Cellular immunity, and T cell-mediated response in particular, plays a fundamental role in controlling Trypanosoma cruzi infection. In chronically infected patients, it has been shown to be involved in progression from the asymptomatic phase of the disease to chronic Chagas cardiopathy. However, the complexity of the parasite and its interaction with the host?s immune system hampers the study of T cell-mediated immune memory. In this piece of research, we undertook the analysis of this response by two different approaches. First, we developed and optimized a protocol for the generation of cell lines by ex vivo expansion of memory T cells from chronic Chagas disease patients, and T. cruzi-specific cultures detection. As a result, we were able to isolate and amplify clonal populations of parasite-specific CD4+ T cells. The second approach involved a bioinformatic prediction and in vitro validation of peptidic epitopes inducing IFN-? secretion on CD4+ and CD8+ T cells from chronic Chagas patients. We thereby found and characterized 7 immunogenic sequences, out of which 4 represent novel T. cruzi epitopes. |
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