Microsoft Word - Manuscrito 25-mar-14.docx
Dehydroleucodine (DhL) is a sesquiterpene lactone isolated from the leaves of the herb Artemisia douglasiana Besser, which grows in Argentina. Previously, our laboratory has shown that DhL inhibited the proliferation of tumor cells. In this work we analyzed the anti-migratory activity of DhL. As st...
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| Lenguaje: | Español |
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2014
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=afitesis&cl=CL1&d=HWA_783 https://repositoriouba.sisbi.uba.ar/gsdl/collect/afitesis/index/assoc/HWA_783.dir/783.PDF |
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| Sumario: | Dehydroleucodine (DhL) is a sesquiterpene lactone isolated from the leaves of the herb Artemisia douglasiana Besser, which grows in Argentina. Previously, our laboratory has shown that DhL inhibited the proliferation of tumor cells. In this work we analyzed the anti-migratory activity of DhL. As study models, tumor cells in culture (HeLa, B16-F0, B16-F10, MCF-7 and MDA-MB-231), arrested in G1/S phase of the cell cycle, were used. In cells treated with DhL were analyzed the properties of adhesion, invasion and migration. DhL did not induce apoptosis or senescence and neither affected the cell viability. DhL inhibited the matrigel invasion and the migration in a wound healing assay. DhL inhibited the membrane protrusion in the cell front due to affected the organization of actin cytoskeleton and inhibited the signalling proteins as Rac1, ERK and p38. These results indicated that DhL, affects the adhesion, invasion and migration of tumor cells in culture due to the inhibition of ERK signalling pathway. This work provides valuable information to consider DhL as a potential pharmacology drug to be used as a antimetastatic agent in cancer therapies. |
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