Polyhydroxyalkanoate degradation is associated with nucleotide accumulation and enhances stress resistance and survival of Pseudomonas oleovorans in natural water microcosms

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Pseudomonas oleovorans GPo1 and its polyhydroxyalkanoic acid (PHA) depolymerization-minus mutant, GPo500 phaZ, residing in natural water microcosms, were utilized to asses the effect of PHA availability on survival and resistance to stress agents. The wild-type strain showed increased survival compa...

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Autores principales:	Ruiz, J.A., López, N.I., Fernández, R.O., Méndez, B.S.
Formato:	Artículo publishedVersion
Publicado:	2001
Materias:	adenosine triphosphate guanosine 3' diphosphate 5' diphosphate nucleotide polyhydroxyalkanoic acid article bacterial growth degradation depolymerization gene mutation genetic complementation nonhuman oxidative phosphorylation phenotype Pseudomonas oleovorans stress survival Biodegradation, Environmental Carboxylic Ester Hydrolases Culture Media Ecosystem Ethanol Fresh Water Heat Mutation Nucleotides Polymers Pseudomonas Bacteria (microorganisms) Equidae Negibacteria
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00992240_v67_n1_p225_Ruiz https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00992240_v67_n1_p225_Ruiz_oai
Aporte de:	Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires

id	I28-R145-paper_00992240_v67_n1_p225_Ruiz_oai
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spelling	I28-R145-paper_00992240_v67_n1_p225_Ruiz_oai2024-08-16 Ruiz, J.A. López, N.I. Fernández, R.O. Méndez, B.S. 2001 Pseudomonas oleovorans GPo1 and its polyhydroxyalkanoic acid (PHA) depolymerization-minus mutant, GPo500 phaZ, residing in natural water microcosms, were utilized to asses the effect of PHA availability on survival and resistance to stress agents. The wild-type strain showed increased survival compared to the PHA depolymerase-minus strain. The appearance of a round cellular shape, characteristic of bacteria growing under starvation conditions, was delayed in the wild type in comparison to the mutant strain. Percent survival at the end of ethanol and heat challenges was always higher in GPo1 than in GPo500. Based on these results and on early experiments (H. Hippe, Arch. Mikrobiol. 56:248-277, 1967) that suggested an association of PHA utilization with respiration and oxidative phosphorylation, we investigated the association between PHA degradation and nucleotide accumulation. ATP and guanosine tetraphosphate (ppGpp) production was analyzed under culture conditions leading to PHA depolymerization. A rise in the ATP and ppGpp levels appeared concomitant with PHA degradation, while this phenomenon was not observed in the mutant strain unable to degrade the polymer. Complementation of the phaZ mutation restored the wild-type phenotype. Fil:Ruiz, J.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_00992240_v67_n1_p225_Ruiz info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar Appl. Environ. Microbiol. 2001;67(1):225-230 adenosine triphosphate guanosine 3' diphosphate 5' diphosphate nucleotide polyhydroxyalkanoic acid article bacterial growth degradation depolymerization gene mutation genetic complementation nonhuman oxidative phosphorylation phenotype Pseudomonas oleovorans stress survival Biodegradation, Environmental Carboxylic Ester Hydrolases Culture Media Ecosystem Ethanol Fresh Water Heat Mutation Nucleotides Polymers Pseudomonas Bacteria (microorganisms) Equidae Negibacteria Pseudomonas Pseudomonas oleovorans Polyhydroxyalkanoate degradation is associated with nucleotide accumulation and enhances stress resistance and survival of Pseudomonas oleovorans in natural water microcosms info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00992240_v67_n1_p225_Ruiz_oai
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-145
collection	Repositorio Digital de la Universidad de Buenos Aires (UBA)
topic	adenosine triphosphate guanosine 3' diphosphate 5' diphosphate nucleotide polyhydroxyalkanoic acid article bacterial growth degradation depolymerization gene mutation genetic complementation nonhuman oxidative phosphorylation phenotype Pseudomonas oleovorans stress survival Biodegradation, Environmental Carboxylic Ester Hydrolases Culture Media Ecosystem Ethanol Fresh Water Heat Mutation Nucleotides Polymers Pseudomonas Bacteria (microorganisms) Equidae Negibacteria Pseudomonas Pseudomonas oleovorans
spellingShingle	adenosine triphosphate guanosine 3' diphosphate 5' diphosphate nucleotide polyhydroxyalkanoic acid article bacterial growth degradation depolymerization gene mutation genetic complementation nonhuman oxidative phosphorylation phenotype Pseudomonas oleovorans stress survival Biodegradation, Environmental Carboxylic Ester Hydrolases Culture Media Ecosystem Ethanol Fresh Water Heat Mutation Nucleotides Polymers Pseudomonas Bacteria (microorganisms) Equidae Negibacteria Pseudomonas Pseudomonas oleovorans Ruiz, J.A. López, N.I. Fernández, R.O. Méndez, B.S. Polyhydroxyalkanoate degradation is associated with nucleotide accumulation and enhances stress resistance and survival of Pseudomonas oleovorans in natural water microcosms
topic_facet	adenosine triphosphate guanosine 3' diphosphate 5' diphosphate nucleotide polyhydroxyalkanoic acid article bacterial growth degradation depolymerization gene mutation genetic complementation nonhuman oxidative phosphorylation phenotype Pseudomonas oleovorans stress survival Biodegradation, Environmental Carboxylic Ester Hydrolases Culture Media Ecosystem Ethanol Fresh Water Heat Mutation Nucleotides Polymers Pseudomonas Bacteria (microorganisms) Equidae Negibacteria Pseudomonas Pseudomonas oleovorans
description	Pseudomonas oleovorans GPo1 and its polyhydroxyalkanoic acid (PHA) depolymerization-minus mutant, GPo500 phaZ, residing in natural water microcosms, were utilized to asses the effect of PHA availability on survival and resistance to stress agents. The wild-type strain showed increased survival compared to the PHA depolymerase-minus strain. The appearance of a round cellular shape, characteristic of bacteria growing under starvation conditions, was delayed in the wild type in comparison to the mutant strain. Percent survival at the end of ethanol and heat challenges was always higher in GPo1 than in GPo500. Based on these results and on early experiments (H. Hippe, Arch. Mikrobiol. 56:248-277, 1967) that suggested an association of PHA utilization with respiration and oxidative phosphorylation, we investigated the association between PHA degradation and nucleotide accumulation. ATP and guanosine tetraphosphate (ppGpp) production was analyzed under culture conditions leading to PHA depolymerization. A rise in the ATP and ppGpp levels appeared concomitant with PHA degradation, while this phenomenon was not observed in the mutant strain unable to degrade the polymer. Complementation of the phaZ mutation restored the wild-type phenotype.
format	Artículo Artículo publishedVersion
author	Ruiz, J.A. López, N.I. Fernández, R.O. Méndez, B.S.
author_facet	Ruiz, J.A. López, N.I. Fernández, R.O. Méndez, B.S.
author_sort	Ruiz, J.A.
title	Polyhydroxyalkanoate degradation is associated with nucleotide accumulation and enhances stress resistance and survival of Pseudomonas oleovorans in natural water microcosms
title_short	Polyhydroxyalkanoate degradation is associated with nucleotide accumulation and enhances stress resistance and survival of Pseudomonas oleovorans in natural water microcosms
title_full	Polyhydroxyalkanoate degradation is associated with nucleotide accumulation and enhances stress resistance and survival of Pseudomonas oleovorans in natural water microcosms
title_fullStr	Polyhydroxyalkanoate degradation is associated with nucleotide accumulation and enhances stress resistance and survival of Pseudomonas oleovorans in natural water microcosms
title_full_unstemmed	Polyhydroxyalkanoate degradation is associated with nucleotide accumulation and enhances stress resistance and survival of Pseudomonas oleovorans in natural water microcosms
title_sort	polyhydroxyalkanoate degradation is associated with nucleotide accumulation and enhances stress resistance and survival of pseudomonas oleovorans in natural water microcosms
publishDate	2001
url	http://hdl.handle.net/20.500.12110/paper_00992240_v67_n1_p225_Ruiz https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00992240_v67_n1_p225_Ruiz_oai
work_keys_str_mv	AT ruizja polyhydroxyalkanoatedegradationisassociatedwithnucleotideaccumulationandenhancesstressresistanceandsurvivalofpseudomonasoleovoransinnaturalwatermicrocosms AT lopezni polyhydroxyalkanoatedegradationisassociatedwithnucleotideaccumulationandenhancesstressresistanceandsurvivalofpseudomonasoleovoransinnaturalwatermicrocosms AT fernandezro polyhydroxyalkanoatedegradationisassociatedwithnucleotideaccumulationandenhancesstressresistanceandsurvivalofpseudomonasoleovoransinnaturalwatermicrocosms AT mendezbs polyhydroxyalkanoatedegradationisassociatedwithnucleotideaccumulationandenhancesstressresistanceandsurvivalofpseudomonasoleovoransinnaturalwatermicrocosms
_version_	1809357087216500736

Polyhydroxyalkanoate degradation is associated with nucleotide accumulation and enhances stress resistance and survival of Pseudomonas oleovorans in natural water microcosms

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