Effect of conjugated linoleic acid on non enzymatic lipid peroxidation of mitochondria obtained from different rat tissues
The polyunsaturated fatty acid composition, chemiluminescence and peroxidizability indexof mitochondria obtained from rat liver, kidney, lung and heart, were studied after oral administrationof conjugated linoleic acid (CLA). After incubation of mitochondria in an ascorbateFe++ system (120 min at 37...
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| Autores principales: | , |
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| Formato: | Artículo revista |
| Lenguaje: | Español |
| Publicado: |
Universidad Nacional del Nordeste
2005
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| Materias: | |
| Acceso en línea: | https://revistas.unne.edu.ar/index.php/vet/article/view/1983 |
| Aporte de: |
| Sumario: | The polyunsaturated fatty acid composition, chemiluminescence and peroxidizability indexof mitochondria obtained from rat liver, kidney, lung and heart, were studied after oral administrationof conjugated linoleic acid (CLA). After incubation of mitochondria in an ascorbateFe++ system (120 min at 37°C), it was observed that the total counts per min/mg protein originatedfrom light emission: chemiluminescence, was lower in liver and kidney mitochondriain the CLA group than in the control group. When peroxidized mitochondrias were comparedto natives, in CLA and control groups it was observed that 18:2 n–6 decreased in all tissuesused in this work. In the control group, it also decrease C20:4 n–6 and C22:6 n–6 in kidneyand liver, while in lung it was affected C22:6 n–3. In CLA group, C20:4 n–6 decreased inliver and kidney, whereas in liver it also decreased C22:6 n–3. As a consequence, the peroxidizabilityindex –a parameter based on the maximal rate of oxidation of fatty acids– showedsignificant changes in liver and kidney mitochondria. These changes were less pronounced inmembranes derived from rats receiving CLA per os. Our results confirm and extend previousobservations that indicated that CLA may act as an antioxidant, protecting membranes fromdeleterious effects. |
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