Molecular diagnosis of infectious laryngotracheitis virus in laying hen farms located in Tungurahua, Ecuador
Avian infectious laryngotracheitis (ILT) is a disease caused by Gallid alphaherpesvirus 1 (GaHV-1), which is transmitted through respiratory secretions or fomites from infected birds, either naturally or through the use of chicken embryo-origin (CEO) live vaccines. The objective of this research was...
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| Formato: | Artículo revista |
| Lenguaje: | Español |
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Universidad Nacional del Nordeste
2024
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| Acceso en línea: | https://revistas.unne.edu.ar/index.php/vet/article/view/7866 |
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I48-R154-article-78662024-11-01T13:19:27Z Molecular diagnosis of infectious laryngotracheitis virus in laying hen farms located in Tungurahua, Ecuador Diagnóstico molecular del virus de laringotraqueítis infecciosa aviar en granjas de gallinas ponedoras localizadas en Tungurahua, Ecuador Revelo-Cueva, M. Vinueza-Burgos, C.V. Metz, G.E. Echeverría, M.G. Commercial hens Sequences Vaccines Thymidine kinase ICP4 Gallinas comerciales Secuencias Vacunas Timidina kinasa ICP4 Avian infectious laryngotracheitis (ILT) is a disease caused by Gallid alphaherpesvirus 1 (GaHV-1), which is transmitted through respiratory secretions or fomites from infected birds, either naturally or through the use of chicken embryo-origin (CEO) live vaccines. The objective of this research was to develop a molecular detection of GaHV-1 in laying hen farms located in Tungurahua Province. Laryngotracheal swabs and tracheal samples were collected from 47 farms. In addition, a recombinant vaccine with GaHV-1 glycoprotein B was used. Three farms tested positive to GaHV-1 as they amplified segments of the ICP4 and thymidine kinase (TK) genes. As expected, the recombinant vaccine analyzed was negative to the amplification of the studies genes. The amplification of the ICP4 and TK genes in the field samples demonstrated the presence of GaHV-1 in the province, but the possibility that it was a vaccine-derived virus from the use of the recombinant vaccine authorized for use in the study area was ruled out. Since the birds did not present clinical signs related to ILT, it was determined that an outbreak of the disease could not be confirmed. However, a molecular study with full genome sequencing is required to obtain conclusive results. However, the use of CEO-type vaccines should be avoided in vaccination schedules in Tungurahua Province, and only the use of vectorized vaccines authorized by the national regulatory authority should continue. La laringotraqueítis infecciosa aviar (ILT) es una enfermedad producida por el Gallid alphaherpesvirus 1 (GaHV-1) que se transmite a través de secreciones respiratorias o fómites de aves infectadas de forma natural o por el uso de vacunas vivas originadas en embrión de pollo (CEO). El objetivo de esta investigación fue detectar molecularmente el GaHV-1 en granjas de gallinas ponedoras localizadas en la provincia de Tungurahua, Ecuador. Se trabajó con hisopados laringotraqueales y muestras de tráqueas de 47 granjas y una vacuna recombinante de uso comercial con la glicoproteína B del GaHV-1. Se encontró que 3 granjas fueron positivas a GaHV-1 al amplificarse segmentos de los genes ICP4 y timidina kinasa (TK) del virus. Por otro lado, la vacuna recombinante analizada resultó negativa a la amplificación de los genes mencionados. La amplificación de los genes ICP4 y TK en las muestras de campo demostró la presencia del GaHV-1 en la provincia, pero se descartó la posibilidad de que se trate de virus vacunal derivado del uso de la vacuna recombinante autorizada para ser usada en la zona de estudio. Dado que las aves no presentaron signos clínicos relacionados con ILT se determinó que no se podría hablar de un brote de la enfermedad, pero se requiere un estudio molecular de secuenciación de genoma completo para obtener resultados concluyentes. No obstante, debería evitarse la utilización de vacunas tipo CEO en los calendarios vacunales en la provincia de Tungurahua y continuar sólo con el uso de las vacunas vectorizadas autorizadas por el ente de control nacional. Universidad Nacional del Nordeste 2024-11-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unne.edu.ar/index.php/vet/article/view/7866 10.30972/vet.3527866 Revista Veterinaria; Vol. 35 Núm. 2 (2024); 74-80 1669-6840 1668-4834 spa https://revistas.unne.edu.ar/index.php/vet/article/view/7866/7388 https://creativecommons.org/licenses/by-nc/4.0 |
| institution |
Universidad Nacional del Nordeste |
| institution_str |
I-48 |
| repository_str |
R-154 |
| container_title_str |
Revistas UNNE - Universidad Nacional del Noroeste (UNNE) |
| language |
Español |
| format |
Artículo revista |
| topic |
Commercial hens Sequences Vaccines Thymidine kinase ICP4 Gallinas comerciales Secuencias Vacunas Timidina kinasa ICP4 |
| spellingShingle |
Commercial hens Sequences Vaccines Thymidine kinase ICP4 Gallinas comerciales Secuencias Vacunas Timidina kinasa ICP4 Revelo-Cueva, M. Vinueza-Burgos, C.V. Metz, G.E. Echeverría, M.G. Molecular diagnosis of infectious laryngotracheitis virus in laying hen farms located in Tungurahua, Ecuador |
| topic_facet |
Commercial hens Sequences Vaccines Thymidine kinase ICP4 Gallinas comerciales Secuencias Vacunas Timidina kinasa ICP4 |
| author |
Revelo-Cueva, M. Vinueza-Burgos, C.V. Metz, G.E. Echeverría, M.G. |
| author_facet |
Revelo-Cueva, M. Vinueza-Burgos, C.V. Metz, G.E. Echeverría, M.G. |
| author_sort |
Revelo-Cueva, M. |
| title |
Molecular diagnosis of infectious laryngotracheitis virus in laying hen farms located in Tungurahua, Ecuador |
| title_short |
Molecular diagnosis of infectious laryngotracheitis virus in laying hen farms located in Tungurahua, Ecuador |
| title_full |
Molecular diagnosis of infectious laryngotracheitis virus in laying hen farms located in Tungurahua, Ecuador |
| title_fullStr |
Molecular diagnosis of infectious laryngotracheitis virus in laying hen farms located in Tungurahua, Ecuador |
| title_full_unstemmed |
Molecular diagnosis of infectious laryngotracheitis virus in laying hen farms located in Tungurahua, Ecuador |
| title_sort |
molecular diagnosis of infectious laryngotracheitis virus in laying hen farms located in tungurahua, ecuador |
| description |
Avian infectious laryngotracheitis (ILT) is a disease caused by Gallid alphaherpesvirus 1 (GaHV-1), which is transmitted through respiratory secretions or fomites from infected birds, either naturally or through the use of chicken embryo-origin (CEO) live vaccines. The objective of this research was to develop a molecular detection of GaHV-1 in laying hen farms located in Tungurahua Province. Laryngotracheal swabs and tracheal samples were collected from 47 farms. In addition, a recombinant vaccine with GaHV-1 glycoprotein B was used. Three farms tested positive to GaHV-1 as they amplified segments of the ICP4 and thymidine kinase (TK) genes. As expected, the recombinant vaccine analyzed was negative to the amplification of the studies genes. The amplification of the ICP4 and TK genes in the field samples demonstrated the presence of GaHV-1 in the province, but the possibility that it was a vaccine-derived virus from the use of the recombinant vaccine authorized for use in the study area was ruled out. Since the birds did not present clinical signs related to ILT, it was determined that an outbreak of the disease could not be confirmed. However, a molecular study with full genome sequencing is required to obtain conclusive results. However, the use of CEO-type vaccines should be avoided in vaccination schedules in Tungurahua Province, and only the use of vectorized vaccines authorized by the national regulatory authority should continue. |
| publisher |
Universidad Nacional del Nordeste |
| publishDate |
2024 |
| url |
https://revistas.unne.edu.ar/index.php/vet/article/view/7866 |
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