Promoter analysis of the Xanthomonas campestris pv. campestris gum operon directing biosynthesis of the xanthan polysaccharide

The Xanthomonas campestris gum gene cluster is composed of 12 genes designated gumB, -C, -D, -E, -F, -G, -H, -I, -J, -K, -L, and -M. The transcriptional organization of this gene cluster was analyzed by the construction of gum-lacZ transcriptional fusions in association with plasmid integration muta...

Descripción completa

Guardado en:
Detalles Bibliográficos
Publicado: 1996
Materias:
xanthan
article
bacterial mutation
carbohydrate synthesis
controlled study
gene cluster
gene mapping
nonhuman
operon
priority journal
promoter region
transcription regulation
transposon
Xanthomonas
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219193_v178_n14_p4313_Katzen
http://hdl.handle.net/20.500.12110/paper_00219193_v178_n14_p4313_Katzen
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:The Xanthomonas campestris gum gene cluster is composed of 12 genes designated gumB, -C, -D, -E, -F, -G, -H, -I, -J, -K, -L, and -M. The transcriptional organization of this gene cluster was analyzed by the construction of gum-lacZ transcriptional fusions in association with plasmid integration mutagenesis. This analysis, coupled with primer extension assays, indicated that the gum region was mainly expressed as an operon from a promoter located upstream of the first gene, gumB.

Ejemplares similares