Differential gene expression promoted by cycloheximide inNeurospora crassa
The effect of a protein synthesis inhibitor on gene expression inNeurospora crassa was analyzed as a model in which sexual differentiation is promoted. Using enriched cDNA probes we isolated several independent genomic clones which contained sequences of genes preferentially expressed when low amoun...
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| Autores principales: | , , , |
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| Publicado: |
1987
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01475975_v11_n2_p122_Aisemberg http://hdl.handle.net/20.500.12110/paper_01475975_v11_n2_p122_Aisemberg |
| Aporte de: |
| Sumario: | The effect of a protein synthesis inhibitor on gene expression inNeurospora crassa was analyzed as a model in which sexual differentiation is promoted. Using enriched cDNA probes we isolated several independent genomic clones which contained sequences of genes preferentially expressed when low amounts of cycloheximide were added to late stationary cultures. These clones hybridized to unique transcripts which belonged to the population of new RNA species generated in cycloheximide-treated mycelia. Several different transcripts were detected with enriched cDNA probes in Northern blots. © 1987 Academic Press, Inc. |
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