Effect of salts on the activity of nitrate reductase from the photosynthetic bacterium Rhodobacter sphaeroides SW

The effect of different salts on the nitrate reductase (NR) activity of Rhodobacter sphaeroides SW was studied. An increase of the activity in the presence of monovalent cations was observed (90, 80 and 77% with Na+, K+ and NH 4+, respectively), whereas divalent cations inhibited the enzyme activity...

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Autor principal: Kerber, Norma Lucía
Publicado: 1995
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0233111X_v35_n1_p47_Paggi
http://hdl.handle.net/20.500.12110/paper_0233111X_v35_n1_p47_Paggi
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_0233111X_v35_n1_p47_Paggi
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spelling paper:paper_0233111X_v35_n1_p47_Paggi2023-06-08T15:21:49Z Effect of salts on the activity of nitrate reductase from the photosynthetic bacterium Rhodobacter sphaeroides SW Kerber, Norma Lucía The effect of different salts on the nitrate reductase (NR) activity of Rhodobacter sphaeroides SW was studied. An increase of the activity in the presence of monovalent cations was observed (90, 80 and 77% with Na+, K+ and NH 4+, respectively), whereas divalent cations inhibited the enzyme activity (30 and 85% with Ca2+ and Mg2+). Both activation or inhibition of the NR activity were reversible. These results could be explained by the interaction of cations with negatively charged amino acids. On the other hand, several anions were also effective in stimulating NR activity (Cl− > NO 3− > Br− > I− > SCN−), suggesting that the degree of hydration of the enzyme molecule might influence its activity. Finally, the effect of salts on the NR activity of R. sphaeroides SW can be atributed to both electrostatic and hydrophobic interactions. Copyright © 1995 Wiley‐VCH Fil:Kerber, N.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1995 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0233111X_v35_n1_p47_Paggi http://hdl.handle.net/20.500.12110/paper_0233111X_v35_n1_p47_Paggi
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
description The effect of different salts on the nitrate reductase (NR) activity of Rhodobacter sphaeroides SW was studied. An increase of the activity in the presence of monovalent cations was observed (90, 80 and 77% with Na+, K+ and NH 4+, respectively), whereas divalent cations inhibited the enzyme activity (30 and 85% with Ca2+ and Mg2+). Both activation or inhibition of the NR activity were reversible. These results could be explained by the interaction of cations with negatively charged amino acids. On the other hand, several anions were also effective in stimulating NR activity (Cl− > NO 3− > Br− > I− > SCN−), suggesting that the degree of hydration of the enzyme molecule might influence its activity. Finally, the effect of salts on the NR activity of R. sphaeroides SW can be atributed to both electrostatic and hydrophobic interactions. Copyright © 1995 Wiley‐VCH
author Kerber, Norma Lucía
spellingShingle Kerber, Norma Lucía
Effect of salts on the activity of nitrate reductase from the photosynthetic bacterium Rhodobacter sphaeroides SW
author_facet Kerber, Norma Lucía
author_sort Kerber, Norma Lucía
title Effect of salts on the activity of nitrate reductase from the photosynthetic bacterium Rhodobacter sphaeroides SW
title_short Effect of salts on the activity of nitrate reductase from the photosynthetic bacterium Rhodobacter sphaeroides SW
title_full Effect of salts on the activity of nitrate reductase from the photosynthetic bacterium Rhodobacter sphaeroides SW
title_fullStr Effect of salts on the activity of nitrate reductase from the photosynthetic bacterium Rhodobacter sphaeroides SW
title_full_unstemmed Effect of salts on the activity of nitrate reductase from the photosynthetic bacterium Rhodobacter sphaeroides SW
title_sort effect of salts on the activity of nitrate reductase from the photosynthetic bacterium rhodobacter sphaeroides sw
publishDate 1995
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0233111X_v35_n1_p47_Paggi
http://hdl.handle.net/20.500.12110/paper_0233111X_v35_n1_p47_Paggi
work_keys_str_mv AT kerbernormalucia effectofsaltsontheactivityofnitratereductasefromthephotosyntheticbacteriumrhodobactersphaeroidessw
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