A single-step purification of an extracellular fungal laccase

A single-step purification procedure for Neurospora crassa laccase is reported. It used Celite chromatography which permits the concentration of the extracellular enzyme from large culture volumes. This method is useful to isolate any fungal or plant laccase as well as other coppercontaining protein...

Descripción completa

Detalles Bibliográficos
Autores principales: Grotewold, Erich, Taccioli, Guillermo, Aisemberg, Gabriel Oscar, Judewicz, Norberto D.
Publicado: 1988
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02650762_v4_n3_p357_Grotewold
http://hdl.handle.net/20.500.12110/paper_02650762_v4_n3_p357_Grotewold
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_02650762_v4_n3_p357_Grotewold
record_format dspace
spelling paper:paper_02650762_v4_n3_p357_Grotewold2023-06-08T15:23:32Z A single-step purification of an extracellular fungal laccase Grotewold, Erich Taccioli, Guillermo Aisemberg, Gabriel Oscar Judewicz, Norberto D. A single-step purification procedure for Neurospora crassa laccase is reported. It used Celite chromatography which permits the concentration of the extracellular enzyme from large culture volumes. This method is useful to isolate any fungal or plant laccase as well as other coppercontaining proteins. This work also takes advantage of the 100-fold induction of the enzyme by low concentrations of cycloheximide. © 1988 Oxford University Press. Fil:Grotewold, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Taccioli, G.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Aisemberg, G.O. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Judewicz, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1988 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02650762_v4_n3_p357_Grotewold http://hdl.handle.net/20.500.12110/paper_02650762_v4_n3_p357_Grotewold
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
description A single-step purification procedure for Neurospora crassa laccase is reported. It used Celite chromatography which permits the concentration of the extracellular enzyme from large culture volumes. This method is useful to isolate any fungal or plant laccase as well as other coppercontaining proteins. This work also takes advantage of the 100-fold induction of the enzyme by low concentrations of cycloheximide. © 1988 Oxford University Press.
author Grotewold, Erich
Taccioli, Guillermo
Aisemberg, Gabriel Oscar
Judewicz, Norberto D.
spellingShingle Grotewold, Erich
Taccioli, Guillermo
Aisemberg, Gabriel Oscar
Judewicz, Norberto D.
A single-step purification of an extracellular fungal laccase
author_facet Grotewold, Erich
Taccioli, Guillermo
Aisemberg, Gabriel Oscar
Judewicz, Norberto D.
author_sort Grotewold, Erich
title A single-step purification of an extracellular fungal laccase
title_short A single-step purification of an extracellular fungal laccase
title_full A single-step purification of an extracellular fungal laccase
title_fullStr A single-step purification of an extracellular fungal laccase
title_full_unstemmed A single-step purification of an extracellular fungal laccase
title_sort single-step purification of an extracellular fungal laccase
publishDate 1988
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02650762_v4_n3_p357_Grotewold
http://hdl.handle.net/20.500.12110/paper_02650762_v4_n3_p357_Grotewold
work_keys_str_mv AT grotewolderich asinglesteppurificationofanextracellularfungallaccase
AT taccioliguillermo asinglesteppurificationofanextracellularfungallaccase
AT aisemberggabrieloscar asinglesteppurificationofanextracellularfungallaccase
AT judewicznorbertod asinglesteppurificationofanextracellularfungallaccase
AT grotewolderich singlesteppurificationofanextracellularfungallaccase
AT taccioliguillermo singlesteppurificationofanextracellularfungallaccase
AT aisemberggabrieloscar singlesteppurificationofanextracellularfungallaccase
AT judewicznorbertod singlesteppurificationofanextracellularfungallaccase
_version_ 1768546539076059136

Ejemplares similares