Effect of 1,5-gluconolactone on the formation of a glucoprotein in potato tuber
The occurrence of a two-step mechanism for the synthesis of a protein bound glucan in potato tuber was confirmed based on a different behaviour of the engaged enzymes towards 1,5-D-gluconolactone. The absence of [14C]erythritol after Smith periodate degradation of the labelled product synthesized du...
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1979
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044211_v15_n2_p151_Tandecarz http://hdl.handle.net/20.500.12110/paper_03044211_v15_n2_p151_Tandecarz |
| Aporte de: |
| Sumario: | The occurrence of a two-step mechanism for the synthesis of a protein bound glucan in potato tuber was confirmed based on a different behaviour of the engaged enzymes towards 1,5-D-gluconolactone. The absence of [14C]erythritol after Smith periodate degradation of the labelled product synthesized during both steps in the presence of 1,5-D-gluconolactone indicated a lack of 1,4 glucosidic linkages. On the contrary, the first step of the reaction, i.e., the formation of a peptidyl-glucose linkage, was not affected by the lactone. These results reinforce the hypothesis of a common mechanism for glucan chain elongation. © 1979. |
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