Porphyrin biosynthesis in Euglena gracilis-VI. The effect of growth conditions on porphobilinogenase activity and further properties

1. 1. The effect of light and growing time on chlorophyll, protein content and the activity of porphobilinogenase (PBGase), in Euglena gracilis was studied. 2. 2. It was found that neither protein nor chlorophyll content or PBGase activity were dependent upon the days of growing or the light conditi...

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Autores principales: Rossetti, María Victoria, Araujo, Lidia Susana, Lombardo, María Elisa, Batlle, Alcira María del Carmen
Publicado: 1987
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03050491_v87_n3_p593_Rossetti
http://hdl.handle.net/20.500.12110/paper_03050491_v87_n3_p593_Rossetti
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spelling paper:paper_03050491_v87_n3_p593_Rossetti2023-06-08T15:30:22Z Porphyrin biosynthesis in Euglena gracilis-VI. The effect of growth conditions on porphobilinogenase activity and further properties Rossetti, María Victoria Araujo, Lidia Susana Lombardo, María Elisa Batlle, Alcira María del Carmen 1. 1. The effect of light and growing time on chlorophyll, protein content and the activity of porphobilinogenase (PBGase), in Euglena gracilis was studied. 2. 2. It was found that neither protein nor chlorophyll content or PBGase activity were dependent upon the days of growing or the light conditions of growth. 3. 3. Some properties of PBGase were studied. The addition of sodium and magnesium salts to the assay system did not modify enzyme activity. Uroporphyrinogen formation was linear with protein over a wide range of enzyme concentration. 4. 4. When activity was expressed on the basis of the amount of porphyrins formed an optimum pH of 7.4 was obtained but when it was expressed in terms of PBG consumed two pH maxima at 7.0 and 8.5 were observed. 5. 5. Direct plots of velocity against PBG concentration, when activity was measured as PBG consumed, were not hyperbolic; double-reciprocal plots were not linear and Hill plots gave a n value of 2, indicating the existence of positive co-operative effects between two binding sites for PBG per molecule of PBGase. © 1987. Fil:Rossetti, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Araujo, L.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lombardo, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:del C. Batlle, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1987 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03050491_v87_n3_p593_Rossetti http://hdl.handle.net/20.500.12110/paper_03050491_v87_n3_p593_Rossetti
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
description 1. 1. The effect of light and growing time on chlorophyll, protein content and the activity of porphobilinogenase (PBGase), in Euglena gracilis was studied. 2. 2. It was found that neither protein nor chlorophyll content or PBGase activity were dependent upon the days of growing or the light conditions of growth. 3. 3. Some properties of PBGase were studied. The addition of sodium and magnesium salts to the assay system did not modify enzyme activity. Uroporphyrinogen formation was linear with protein over a wide range of enzyme concentration. 4. 4. When activity was expressed on the basis of the amount of porphyrins formed an optimum pH of 7.4 was obtained but when it was expressed in terms of PBG consumed two pH maxima at 7.0 and 8.5 were observed. 5. 5. Direct plots of velocity against PBG concentration, when activity was measured as PBG consumed, were not hyperbolic; double-reciprocal plots were not linear and Hill plots gave a n value of 2, indicating the existence of positive co-operative effects between two binding sites for PBG per molecule of PBGase. © 1987.
author Rossetti, María Victoria
Araujo, Lidia Susana
Lombardo, María Elisa
Batlle, Alcira María del Carmen
spellingShingle Rossetti, María Victoria
Araujo, Lidia Susana
Lombardo, María Elisa
Batlle, Alcira María del Carmen
Porphyrin biosynthesis in Euglena gracilis-VI. The effect of growth conditions on porphobilinogenase activity and further properties
author_facet Rossetti, María Victoria
Araujo, Lidia Susana
Lombardo, María Elisa
Batlle, Alcira María del Carmen
author_sort Rossetti, María Victoria
title Porphyrin biosynthesis in Euglena gracilis-VI. The effect of growth conditions on porphobilinogenase activity and further properties
title_short Porphyrin biosynthesis in Euglena gracilis-VI. The effect of growth conditions on porphobilinogenase activity and further properties
title_full Porphyrin biosynthesis in Euglena gracilis-VI. The effect of growth conditions on porphobilinogenase activity and further properties
title_fullStr Porphyrin biosynthesis in Euglena gracilis-VI. The effect of growth conditions on porphobilinogenase activity and further properties
title_full_unstemmed Porphyrin biosynthesis in Euglena gracilis-VI. The effect of growth conditions on porphobilinogenase activity and further properties
title_sort porphyrin biosynthesis in euglena gracilis-vi. the effect of growth conditions on porphobilinogenase activity and further properties
publishDate 1987
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03050491_v87_n3_p593_Rossetti
http://hdl.handle.net/20.500.12110/paper_03050491_v87_n3_p593_Rossetti
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