Porphyrin biosynthesis in Rhodopseudomonas palustris-VIII. Purification and properties of deaminase

1. 1. Uroporphyrinogen I synthetase from Rhodopseudomonas palustris has been isolated and purified. 2. 2. Assay conditions were determined. 3. 3. Sephadex G-100 column chromatography was found to increase 4-fold the degree of purification yielding a deaminase that was purified 72-fold. 4. 4. Some pr...

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Detalles Bibliográficos
Autores principales: Kotler, Mónica Lidia, Fumagalli, Silvia Ana, Juknat, Adela Ana, Batlle, Alcira María del Carmen
Publicado: 1987
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03050491_v87_n3_p601_Kotler
http://hdl.handle.net/20.500.12110/paper_03050491_v87_n3_p601_Kotler
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_03050491_v87_n3_p601_Kotler
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spelling paper:paper_03050491_v87_n3_p601_Kotler2023-06-08T15:30:23Z Porphyrin biosynthesis in Rhodopseudomonas palustris-VIII. Purification and properties of deaminase Kotler, Mónica Lidia Fumagalli, Silvia Ana Juknat, Adela Ana Batlle, Alcira María del Carmen 1. 1. Uroporphyrinogen I synthetase from Rhodopseudomonas palustris has been isolated and purified. 2. 2. Assay conditions were determined. 3. 3. Sephadex G-100 column chromatography was found to increase 4-fold the degree of purification yielding a deaminase that was purified 72-fold. 4. 4. Some properties of the isolated enzyme were studied. The optimal pH was about 7.6-7.8. Porphyrin formation was linear with time. The presence of several thiol reagents was found to be no essential for deaminase activity. 5. 5. Deaminase exhibited classical Michaelis-Menten kinetics Km and Vmax were estimated. 6. 6. Molecular weight determinations, by means of gel filtration on a calibrated Sephadex G-100 column, gave values of 74,000 ± 7400. © 1987. Fil:Kotler, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fumagalli, S.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Juknat, A.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:del C. Batlle, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1987 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03050491_v87_n3_p601_Kotler http://hdl.handle.net/20.500.12110/paper_03050491_v87_n3_p601_Kotler
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
description 1. 1. Uroporphyrinogen I synthetase from Rhodopseudomonas palustris has been isolated and purified. 2. 2. Assay conditions were determined. 3. 3. Sephadex G-100 column chromatography was found to increase 4-fold the degree of purification yielding a deaminase that was purified 72-fold. 4. 4. Some properties of the isolated enzyme were studied. The optimal pH was about 7.6-7.8. Porphyrin formation was linear with time. The presence of several thiol reagents was found to be no essential for deaminase activity. 5. 5. Deaminase exhibited classical Michaelis-Menten kinetics Km and Vmax were estimated. 6. 6. Molecular weight determinations, by means of gel filtration on a calibrated Sephadex G-100 column, gave values of 74,000 ± 7400. © 1987.
author Kotler, Mónica Lidia
Fumagalli, Silvia Ana
Juknat, Adela Ana
Batlle, Alcira María del Carmen
spellingShingle Kotler, Mónica Lidia
Fumagalli, Silvia Ana
Juknat, Adela Ana
Batlle, Alcira María del Carmen
Porphyrin biosynthesis in Rhodopseudomonas palustris-VIII. Purification and properties of deaminase
author_facet Kotler, Mónica Lidia
Fumagalli, Silvia Ana
Juknat, Adela Ana
Batlle, Alcira María del Carmen
author_sort Kotler, Mónica Lidia
title Porphyrin biosynthesis in Rhodopseudomonas palustris-VIII. Purification and properties of deaminase
title_short Porphyrin biosynthesis in Rhodopseudomonas palustris-VIII. Purification and properties of deaminase
title_full Porphyrin biosynthesis in Rhodopseudomonas palustris-VIII. Purification and properties of deaminase
title_fullStr Porphyrin biosynthesis in Rhodopseudomonas palustris-VIII. Purification and properties of deaminase
title_full_unstemmed Porphyrin biosynthesis in Rhodopseudomonas palustris-VIII. Purification and properties of deaminase
title_sort porphyrin biosynthesis in rhodopseudomonas palustris-viii. purification and properties of deaminase
publishDate 1987
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03050491_v87_n3_p601_Kotler
http://hdl.handle.net/20.500.12110/paper_03050491_v87_n3_p601_Kotler
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