An α(D)-globin fragment from Triatoma infestans hindgut stimulates Trypanosoma cruzi adenylyl cyclase and promotes metacyclogenesis

A peptide from hindguts of the Triatoma infestans, the hematophagous Chagus' insect vector, activates adenylyl cyclase activity in Trypanosoma cruzi epimastigote membranes and stimulates the in vitro differentiation of epimastigotes (proliferative and non-infectious forms) to metacyclic trypoma...

Descripción completa

Guardado en:
Detalles Bibliográficos
Publicado: 1993
Materias:
adenylate cyclase
alpha globin
peptide
animal tissue
conference paper
enzyme activation
epimastigote
insect
intestine
nonhuman
trypanosoma cruzi
Adenylate Cyclase
Amino Acid Sequence
Animal
Chickens
Digestive Physiology
Globins
Mice
Molecular Sequence Data
Peptide Fragments
Support, Non-U.S. Gov't
Triatoma
Trypanosoma cruzi
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07169760_v26_n1-2_p279_Fraidenraich
http://hdl.handle.net/20.500.12110/paper_07169760_v26_n1-2_p279_Fraidenraich
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:A peptide from hindguts of the Triatoma infestans, the hematophagous Chagus' insect vector, activates adenylyl cyclase activity in Trypanosoma cruzi epimastigote membranes and stimulates the in vitro differentiation of epimastigotes (proliferative and non-infectious forms) to metacyclic trypomastigotes (non-proliferative and infectious forms). The peptide was purified from hindguts of insects fed two days before with chicken blood. After purification, the peptide showed upon SDS-PAGE a single band of about 10 kDa. The sequence for 20 residues of the amino terminus of this peptide was: H2N-Met-Leu-Thr-Ala-Glu-Asp-Lys-Lys-Leu-Ile-Gln-Gln-Ala-Trp-Glu-Lys-A la-Ala-Ser-His. This sequence corresponds to the amino terminus of chicken α(D)-globin. A synthetic peptide with the sequence of the 40 amino acids corresponding to the amino terminus of α(D)-globin, also stimulated T. cruzi adenylyl cyclase activity and promoted metacyclogenesis.

Ejemplares similares