Layer-by-layer self-assembly of glucose oxidase with a poly(allylamine)ferrocene redox mediator

We report the redox mediation of glucose oxidase (GOx) in a self-assembled structure of cationic poly-(allylamine) modified by ferrocene (PAA-Fc) and anionic GOx deposited electrostatically layer-by-layer on negatively charged alkanethiol-modified Au surfaces. Successive PAA-Fc and GOx layers were d...

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Detalles Bibliográficos
Autores principales: Hodak, Jose Hector, Etchenique, Roberto, Calvo, Ernesto Julio
Publicado: 1997
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07437463_v13_n10_p2708_Hodak
http://hdl.handle.net/20.500.12110/paper_07437463_v13_n10_p2708_Hodak
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_07437463_v13_n10_p2708_Hodak
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spelling paper:paper_07437463_v13_n10_p2708_Hodak2023-06-08T15:44:48Z Layer-by-layer self-assembly of glucose oxidase with a poly(allylamine)ferrocene redox mediator Hodak, Jose Hector Etchenique, Roberto Calvo, Ernesto Julio We report the redox mediation of glucose oxidase (GOx) in a self-assembled structure of cationic poly-(allylamine) modified by ferrocene (PAA-Fc) and anionic GOx deposited electrostatically layer-by-layer on negatively charged alkanethiol-modified Au surfaces. Successive PAA-Fc and GOx layers were deposited by alternate immersion of the thiol-modified Au in the respective polyelectrolyte and enzyme solutions. The uptake of thiol, redox polymer, and GOx on the surface was monitored by quartz crystal microbalance. Cyclic voltammetry shows nearly ideal surface waves of ferrocene in the polymer with charge independent of sweep rate; the redox surface concentration was obtained from integration of the ferrocene/ferricinium voltammetric peaks. The redox charge increases in step with the number of PAA-Fc layers deposited. Enzyme catalysis for the oxidation of β-D-glucose was achieved with a multilayer PAA-Fc/GOx assembly, with each GOx layer contributing equally to the catalytic response. Only a small fraction of the active assembled GOx molecules are "electrically wired" by the ferrocene polymer although all of the enzyme could be oxidized by soluble ferrocenesulfonate when added to solution. Fil:Hodak, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Etchenique, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calvo, E.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1997 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07437463_v13_n10_p2708_Hodak http://hdl.handle.net/20.500.12110/paper_07437463_v13_n10_p2708_Hodak
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
description We report the redox mediation of glucose oxidase (GOx) in a self-assembled structure of cationic poly-(allylamine) modified by ferrocene (PAA-Fc) and anionic GOx deposited electrostatically layer-by-layer on negatively charged alkanethiol-modified Au surfaces. Successive PAA-Fc and GOx layers were deposited by alternate immersion of the thiol-modified Au in the respective polyelectrolyte and enzyme solutions. The uptake of thiol, redox polymer, and GOx on the surface was monitored by quartz crystal microbalance. Cyclic voltammetry shows nearly ideal surface waves of ferrocene in the polymer with charge independent of sweep rate; the redox surface concentration was obtained from integration of the ferrocene/ferricinium voltammetric peaks. The redox charge increases in step with the number of PAA-Fc layers deposited. Enzyme catalysis for the oxidation of β-D-glucose was achieved with a multilayer PAA-Fc/GOx assembly, with each GOx layer contributing equally to the catalytic response. Only a small fraction of the active assembled GOx molecules are "electrically wired" by the ferrocene polymer although all of the enzyme could be oxidized by soluble ferrocenesulfonate when added to solution.
author Hodak, Jose Hector
Etchenique, Roberto
Calvo, Ernesto Julio
spellingShingle Hodak, Jose Hector
Etchenique, Roberto
Calvo, Ernesto Julio
Layer-by-layer self-assembly of glucose oxidase with a poly(allylamine)ferrocene redox mediator
author_facet Hodak, Jose Hector
Etchenique, Roberto
Calvo, Ernesto Julio
author_sort Hodak, Jose Hector
title Layer-by-layer self-assembly of glucose oxidase with a poly(allylamine)ferrocene redox mediator
title_short Layer-by-layer self-assembly of glucose oxidase with a poly(allylamine)ferrocene redox mediator
title_full Layer-by-layer self-assembly of glucose oxidase with a poly(allylamine)ferrocene redox mediator
title_fullStr Layer-by-layer self-assembly of glucose oxidase with a poly(allylamine)ferrocene redox mediator
title_full_unstemmed Layer-by-layer self-assembly of glucose oxidase with a poly(allylamine)ferrocene redox mediator
title_sort layer-by-layer self-assembly of glucose oxidase with a poly(allylamine)ferrocene redox mediator
publishDate 1997
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07437463_v13_n10_p2708_Hodak
http://hdl.handle.net/20.500.12110/paper_07437463_v13_n10_p2708_Hodak
work_keys_str_mv AT hodakjosehector layerbylayerselfassemblyofglucoseoxidasewithapolyallylamineferroceneredoxmediator
AT etcheniqueroberto layerbylayerselfassemblyofglucoseoxidasewithapolyallylamineferroceneredoxmediator
AT calvoernestojulio layerbylayerselfassemblyofglucoseoxidasewithapolyallylamineferroceneredoxmediator
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