First morphological and molecular analysis of Eucoleus boehmi like eggs in dogs from Argentina

The canid parasites Eucoleus aerophilus (syn. Capillaria aerophila) and Eucoleus boehmi (syn. Capillaria boehmi) parasitize the lower and the upper respiratory tract, respectively. Reports and descriptions of these nematodes are scarce in Argentina, possibly due to misdiagnosis of morphologically si...

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Detalles Bibliográficos
Publicado: 2018
Materias:
Argentina
cox 1 gene
Eucoleus boehmi eggs
Scanning electron microscope
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09320113_v_n_p1_Lavallen
http://hdl.handle.net/20.500.12110/paper_09320113_v_n_p1_Lavallen
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_09320113_v_n_p1_Lavallen
record_format dspace
spelling paper:paper_09320113_v_n_p1_Lavallen2023-06-08T15:53:03Z First morphological and molecular analysis of Eucoleus boehmi like eggs in dogs from Argentina Argentina cox 1 gene Eucoleus boehmi eggs Scanning electron microscope The canid parasites Eucoleus aerophilus (syn. Capillaria aerophila) and Eucoleus boehmi (syn. Capillaria boehmi) parasitize the lower and the upper respiratory tract, respectively. Reports and descriptions of these nematodes are scarce in Argentina, possibly due to misdiagnosis of morphologically similar trichuroids eggs, and the lack of knowledge about the species of Eucoleus in this geographical area. Scanning electron microscopy is a useful tool for identification of E. boehmi eggs based on the characteristics of the shell structure which differentiate between species. Molecular analysis complements morphological identification. Until now, there are no studies based on the analysis of E. boehmi eggs in Argentina. The aim of the present work was to study by morphological, morphometric, and molecular analysis, eggs attributable to E. boehmi isolated from dogs naturally infected in Mar del Plata city, Argentina. Eggs isolated from two dog fecal samples were analyzed by light and scanning electron microscopy. A fragment of mitochondrial DNA (mtDNA) of the cytochrome c oxidase subunit I gene (cox1) from eggs was sequenced, and phylogenetic analysis was performed in this study. According to morphological results based on the wall surface ultrastructure, the eggs studied were assigned to E. boehmi. Molecular analysis supported the morphological identification. The divergence of 9–12% with the European isolated could suggest a new geographical genetic variation of E. boehmi, but also question the possible existence of cryptic species. This is the first characterization of E. boehmi eggs in dogs from Argentina. © 2018 Springer-Verlag GmbH Germany, part of Springer Nature 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09320113_v_n_p1_Lavallen http://hdl.handle.net/20.500.12110/paper_09320113_v_n_p1_Lavallen
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Argentina
cox 1 gene
Eucoleus boehmi eggs
Scanning electron microscope
spellingShingle Argentina
cox 1 gene
Eucoleus boehmi eggs
Scanning electron microscope
First morphological and molecular analysis of Eucoleus boehmi like eggs in dogs from Argentina
topic_facet Argentina
cox 1 gene
Eucoleus boehmi eggs
Scanning electron microscope
description The canid parasites Eucoleus aerophilus (syn. Capillaria aerophila) and Eucoleus boehmi (syn. Capillaria boehmi) parasitize the lower and the upper respiratory tract, respectively. Reports and descriptions of these nematodes are scarce in Argentina, possibly due to misdiagnosis of morphologically similar trichuroids eggs, and the lack of knowledge about the species of Eucoleus in this geographical area. Scanning electron microscopy is a useful tool for identification of E. boehmi eggs based on the characteristics of the shell structure which differentiate between species. Molecular analysis complements morphological identification. Until now, there are no studies based on the analysis of E. boehmi eggs in Argentina. The aim of the present work was to study by morphological, morphometric, and molecular analysis, eggs attributable to E. boehmi isolated from dogs naturally infected in Mar del Plata city, Argentina. Eggs isolated from two dog fecal samples were analyzed by light and scanning electron microscopy. A fragment of mitochondrial DNA (mtDNA) of the cytochrome c oxidase subunit I gene (cox1) from eggs was sequenced, and phylogenetic analysis was performed in this study. According to morphological results based on the wall surface ultrastructure, the eggs studied were assigned to E. boehmi. Molecular analysis supported the morphological identification. The divergence of 9–12% with the European isolated could suggest a new geographical genetic variation of E. boehmi, but also question the possible existence of cryptic species. This is the first characterization of E. boehmi eggs in dogs from Argentina. © 2018 Springer-Verlag GmbH Germany, part of Springer Nature
title First morphological and molecular analysis of Eucoleus boehmi like eggs in dogs from Argentina
title_short First morphological and molecular analysis of Eucoleus boehmi like eggs in dogs from Argentina
title_full First morphological and molecular analysis of Eucoleus boehmi like eggs in dogs from Argentina
title_fullStr First morphological and molecular analysis of Eucoleus boehmi like eggs in dogs from Argentina
title_full_unstemmed First morphological and molecular analysis of Eucoleus boehmi like eggs in dogs from Argentina
title_sort first morphological and molecular analysis of eucoleus boehmi like eggs in dogs from argentina
publishDate 2018
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09320113_v_n_p1_Lavallen
http://hdl.handle.net/20.500.12110/paper_09320113_v_n_p1_Lavallen
_version_ 1768543091681132544

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