Standardization of fixation, processing and staining methods for the central nervous system of vertebrates

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This paper reports the standardization of methods used for processing and embedding various vertebrate brains of different sizes in paraffin. Other technical details developed for avoiding frequent difficulties arising during laboratory routine are also reported. Some modifications of the Nissl and...

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Detalles Bibliográficos
Autores principales: Aldana Marcos, H.J., Ferrari, C.C., Benitez, I., Affanni, J.M.
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: 1996
Materias:
Klüver-Barrera
Microwave oven
Neuroanatomy
Nissl
Paraffin sections
coloring agent
animal
article
central nervous system
histology
laboratory diagnosis
methodology
microtomy
staining
standard
tissue fixation
vertebrate
Animals
Central Nervous System
Coloring Agents
Microtomy
Specimen Handling
Staining and Labeling
Tissue Embedding
Tissue Fixation
Vertebrates
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03279545_v20_n3_p265_AldanaMarcos
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paperaa:paper_03279545_v20_n3_p265_AldanaMarcos
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spelling paperaa:paper_03279545_v20_n3_p265_AldanaMarcos2023-06-12T16:47:39Z Standardization of fixation, processing and staining methods for the central nervous system of vertebrates Biocell 1996;20(3):265-272 Aldana Marcos, H.J. Ferrari, C.C. Benitez, I. Affanni, J.M. Klüver-Barrera Microwave oven Neuroanatomy Nissl Paraffin sections coloring agent animal article central nervous system histology laboratory diagnosis methodology microtomy staining standard tissue fixation vertebrate Animals Central Nervous System Coloring Agents Microtomy Specimen Handling Staining and Labeling Tissue Embedding Tissue Fixation Vertebrates This paper reports the standardization of methods used for processing and embedding various vertebrate brains of different sizes in paraffin. Other technical details developed for avoiding frequent difficulties arising during laboratory routine are also reported. Some modifications of the Nissl and Klüver-Barrera staining methods are proposed. These modifications include: 1) a Nissl stain solution with a rapid and efficient action with easier differentiation; 2) the use of a cheap microwave oven for the Klüver-Barrera stain. These procedures have the advantage of permitting Nissl and Klüver-Barrera staining of nervous tissue in about five and fifteen minutes respectively. The proposed procedures have been tested in brains obtained from fish, amphibians, reptiles and mammals of different body sizes. They are the result of our long experience in preparing slides for comparative studies. Serial sections of excellent quality were regularly obtained in all the specimens studied. These standardized methods, being simple and quick, are recommended for routine use in neurobiological laboratories. Fil:Aldana Marcos, H.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ferrari, C.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1996 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03279545_v20_n3_p265_AldanaMarcos
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language Inglés
orig_language_str_mv eng
topic Klüver-Barrera
Microwave oven
Neuroanatomy
Nissl
Paraffin sections
coloring agent
animal
article
central nervous system
histology
laboratory diagnosis
methodology
microtomy
staining
standard
tissue fixation
vertebrate
Animals
Central Nervous System
Coloring Agents
Microtomy
Specimen Handling
Staining and Labeling
Tissue Embedding
Tissue Fixation
Vertebrates
spellingShingle Klüver-Barrera
Microwave oven
Neuroanatomy
Nissl
Paraffin sections
coloring agent
animal
article
central nervous system
histology
laboratory diagnosis
methodology
microtomy
staining
standard
tissue fixation
vertebrate
Animals
Central Nervous System
Coloring Agents
Microtomy
Specimen Handling
Staining and Labeling
Tissue Embedding
Tissue Fixation
Vertebrates
Aldana Marcos, H.J.
Ferrari, C.C.
Benitez, I.
Affanni, J.M.
Standardization of fixation, processing and staining methods for the central nervous system of vertebrates
topic_facet Klüver-Barrera
Microwave oven
Neuroanatomy
Nissl
Paraffin sections
coloring agent
animal
article
central nervous system
histology
laboratory diagnosis
methodology
microtomy
staining
standard
tissue fixation
vertebrate
Animals
Central Nervous System
Coloring Agents
Microtomy
Specimen Handling
Staining and Labeling
Tissue Embedding
Tissue Fixation
Vertebrates
description This paper reports the standardization of methods used for processing and embedding various vertebrate brains of different sizes in paraffin. Other technical details developed for avoiding frequent difficulties arising during laboratory routine are also reported. Some modifications of the Nissl and Klüver-Barrera staining methods are proposed. These modifications include: 1) a Nissl stain solution with a rapid and efficient action with easier differentiation; 2) the use of a cheap microwave oven for the Klüver-Barrera stain. These procedures have the advantage of permitting Nissl and Klüver-Barrera staining of nervous tissue in about five and fifteen minutes respectively. The proposed procedures have been tested in brains obtained from fish, amphibians, reptiles and mammals of different body sizes. They are the result of our long experience in preparing slides for comparative studies. Serial sections of excellent quality were regularly obtained in all the specimens studied. These standardized methods, being simple and quick, are recommended for routine use in neurobiological laboratories.
format Artículo
Artículo
publishedVersion
author Aldana Marcos, H.J.
Ferrari, C.C.
Benitez, I.
Affanni, J.M.
author_facet Aldana Marcos, H.J.
Ferrari, C.C.
Benitez, I.
Affanni, J.M.
author_sort Aldana Marcos, H.J.
title Standardization of fixation, processing and staining methods for the central nervous system of vertebrates
title_short Standardization of fixation, processing and staining methods for the central nervous system of vertebrates
title_full Standardization of fixation, processing and staining methods for the central nervous system of vertebrates
title_fullStr Standardization of fixation, processing and staining methods for the central nervous system of vertebrates
title_full_unstemmed Standardization of fixation, processing and staining methods for the central nervous system of vertebrates
title_sort standardization of fixation, processing and staining methods for the central nervous system of vertebrates
publishDate 1996
url http://hdl.handle.net/20.500.12110/paper_03279545_v20_n3_p265_AldanaMarcos
work_keys_str_mv AT aldanamarcoshj standardizationoffixationprocessingandstainingmethodsforthecentralnervoussystemofvertebrates
AT ferraricc standardizationoffixationprocessingandstainingmethodsforthecentralnervoussystemofvertebrates
AT benitezi standardizationoffixationprocessingandstainingmethodsforthecentralnervoussystemofvertebrates
AT affannijm standardizationoffixationprocessingandstainingmethodsforthecentralnervoussystemofvertebrates
_version_ 1769810032120037376

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