A specific method for the quantitative determination of β-glucose-1-phosphate

A specific spectrophotometric determination of β-glucose-1-phosphate has been devised. It allows β-glucose-1-phosphate to be measured in the presence of α-glucose-1-phosphate and of a one hundred-fold excess of glucose-6-phosphate. Phosphoglucomutase for β-glucose-1-phosphate obtained from cells of...

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Detalles Bibliográficos
Autores principales: Belocopitow, E., Maréchal, L.R.
Formato: JOUR
Materias:
glucose 1 phosphate
phosphoglucomutase
methodology
Animal
Arsenic
Borohydrides
Enzyme Activation
Euglena gracilis
Glucosephosphates
Hydrogen-Ion Concentration
Kinetics
Methods
Phosphoglucomutase
Precipitation
Protamines
Stereoisomerism
Sulfuric Acids
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00032697_v53_n1_p108_Belocopitow
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:A specific spectrophotometric determination of β-glucose-1-phosphate has been devised. It allows β-glucose-1-phosphate to be measured in the presence of α-glucose-1-phosphate and of a one hundred-fold excess of glucose-6-phosphate. Phosphoglucomutase for β-glucose-1-phosphate obtained from cells of Euglena gracilis var. bacillaris must be prepared for the assay. © 1973.

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