Microscale Identification of Several Sugar Phosphates by Paper Chromatography and Electrophoresis

Several sugar phosphates have been converted to cyclic phosphates or methyl esters by treatment with dicyclohexylcarbodiimide in methanolic solution. The products obtained were then separated by paper chromatography with solvents containing cetyltrimethylammonium bromide or by paper electrophoresis...

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Detalles Bibliográficos
Autores principales: Piras, R., Cabib, E.
Formato: JOUR
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00032700_v35_n6_p755_Piras
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00032700_v35_n6_p755_Piras
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spelling todo:paper_00032700_v35_n6_p755_Piras2023-10-03T13:55:57Z Microscale Identification of Several Sugar Phosphates by Paper Chromatography and Electrophoresis Piras, R. Cabib, E. Several sugar phosphates have been converted to cyclic phosphates or methyl esters by treatment with dicyclohexylcarbodiimide in methanolic solution. The products obtained were then separated by paper chromatography with solvents containing cetyltrimethylammonium bromide or by paper electrophoresis in potassium or cetyltrimethylammonium borate buffers. The separations obtained were better than those previously described for the untreated esters and the complete procedure could be carried out with less than 0.5 μmole of substance. The compounds obtained in the dicyclohexylcarbodiimide reaction were studied in detail and general rules have been formulated for the prediction of the type of product to be expected in each case. Some correlations between chemical structure and chromatographic or electrophoretic behavior have been observed. © 1963, American Chemical Society. All rights reserved. Fil:Cabib, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00032700_v35_n6_p755_Piras
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
description Several sugar phosphates have been converted to cyclic phosphates or methyl esters by treatment with dicyclohexylcarbodiimide in methanolic solution. The products obtained were then separated by paper chromatography with solvents containing cetyltrimethylammonium bromide or by paper electrophoresis in potassium or cetyltrimethylammonium borate buffers. The separations obtained were better than those previously described for the untreated esters and the complete procedure could be carried out with less than 0.5 μmole of substance. The compounds obtained in the dicyclohexylcarbodiimide reaction were studied in detail and general rules have been formulated for the prediction of the type of product to be expected in each case. Some correlations between chemical structure and chromatographic or electrophoretic behavior have been observed. © 1963, American Chemical Society. All rights reserved.
format JOUR
author Piras, R.
Cabib, E.
spellingShingle Piras, R.
Cabib, E.
Microscale Identification of Several Sugar Phosphates by Paper Chromatography and Electrophoresis
author_facet Piras, R.
Cabib, E.
author_sort Piras, R.
title Microscale Identification of Several Sugar Phosphates by Paper Chromatography and Electrophoresis
title_short Microscale Identification of Several Sugar Phosphates by Paper Chromatography and Electrophoresis
title_full Microscale Identification of Several Sugar Phosphates by Paper Chromatography and Electrophoresis
title_fullStr Microscale Identification of Several Sugar Phosphates by Paper Chromatography and Electrophoresis
title_full_unstemmed Microscale Identification of Several Sugar Phosphates by Paper Chromatography and Electrophoresis
title_sort microscale identification of several sugar phosphates by paper chromatography and electrophoresis
url http://hdl.handle.net/20.500.12110/paper_00032700_v35_n6_p755_Piras
work_keys_str_mv AT pirasr microscaleidentificationofseveralsugarphosphatesbypaperchromatographyandelectrophoresis
AT cabibe microscaleidentificationofseveralsugarphosphatesbypaperchromatographyandelectrophoresis
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