Surfactant-assisted lipopolysaccharide conjugation employing a cyanopyridinium agent and its application to a competitive assay

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The activation of a lipopolysaccharide (LPS) with 1-cyano-4- dimethylaminopyridinium tetrafluoroborate (CDAP) in the presence of a surfactant allows an efficient conjugation with dansyl hydrazine or horseradish peroxidase (HRP) in an aqueous medium maintaining its biological activity. In order to pr...

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Detalles Bibliográficos
Autores principales:	Pallarola, D., Battaglini, F.
Formato:	JOUR
Materias:	Amperometric Amphiphilic compound Aqueous medium Biological activities Electrode arrays Enzymatic activities Horseradish peroxidase HRP conjugate Hydrazide groups Lipopolysaccharide Lipopolysaccharides Simultaneous determinations Sodium deoxycholate Tetrafluoroborate Amines Hydrazine Sodium Surface active agents Polysaccharides 1 cyano 4 dimethylaminopyridinium tetrafluoroborate deoxycholate sodium lipopolysaccharide pyridinium derivative unclassified drug article bacterial strain chemical reaction chemical structure concentration (parameters) controlled study enzyme activity nonhuman pH measurement Binding, Competitive Dansyl Compounds Electrochemical Techniques Electrodes Horseradish Peroxidase Hydrazines Nitriles Pyridinium Compounds Surface-Active Agents
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00032700_v81_n10_p3824_Pallarola
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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