Structural studies on a glycopeptide from the tree fungus Cyttaria harioti Fischer

A glycopeptide (In1) was isolated by phenol-water extraction from Cyttaria harioti Fischer, parasite of Nothofagus sps. Neutral sugars account for 89% of In1 and were characterized as glucose, mannose, and galactose. Glucosamine, identified by g.l.c., was colorimetrically estimated (5.8%). The molar...

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Autores principales: Oliva, E.M., Cirelli, A.F., de Lederkremer, R.M.
Formato: JOUR
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00086215_v138_n2_p257_Oliva
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_00086215_v138_n2_p257_Oliva2023-10-03T14:06:19Z Structural studies on a glycopeptide from the tree fungus Cyttaria harioti Fischer Oliva, E.M. Cirelli, A.F. de Lederkremer, R.M. A glycopeptide (In1) was isolated by phenol-water extraction from Cyttaria harioti Fischer, parasite of Nothofagus sps. Neutral sugars account for 89% of In1 and were characterized as glucose, mannose, and galactose. Glucosamine, identified by g.l.c., was colorimetrically estimated (5.8%). The molar ratio of Glc:Man:Gal:GlcNAc was 17:11:3:2. The linkages between the various monosaccharide residues were established through methylation analysis and periodate oxidation studies. The anomeric configurations of the various glycosyl groups were determined by chromium trioxide oxidation of the acetylated polysaccharide. The results were confirmed by 13C-n.m.r. spectroscopy. The sugar chain is N-glycosyl-linked to the peptide. Structural features of the carbohydrate moiety of glycopeptide In1 are described. © 1985. Fil:Oliva, E.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:de Lederkremer, R.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00086215_v138_n2_p257_Oliva
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
description A glycopeptide (In1) was isolated by phenol-water extraction from Cyttaria harioti Fischer, parasite of Nothofagus sps. Neutral sugars account for 89% of In1 and were characterized as glucose, mannose, and galactose. Glucosamine, identified by g.l.c., was colorimetrically estimated (5.8%). The molar ratio of Glc:Man:Gal:GlcNAc was 17:11:3:2. The linkages between the various monosaccharide residues were established through methylation analysis and periodate oxidation studies. The anomeric configurations of the various glycosyl groups were determined by chromium trioxide oxidation of the acetylated polysaccharide. The results were confirmed by 13C-n.m.r. spectroscopy. The sugar chain is N-glycosyl-linked to the peptide. Structural features of the carbohydrate moiety of glycopeptide In1 are described. © 1985.
format JOUR
author Oliva, E.M.
Cirelli, A.F.
de Lederkremer, R.M.
spellingShingle Oliva, E.M.
Cirelli, A.F.
de Lederkremer, R.M.
Structural studies on a glycopeptide from the tree fungus Cyttaria harioti Fischer
author_facet Oliva, E.M.
Cirelli, A.F.
de Lederkremer, R.M.
author_sort Oliva, E.M.
title Structural studies on a glycopeptide from the tree fungus Cyttaria harioti Fischer
title_short Structural studies on a glycopeptide from the tree fungus Cyttaria harioti Fischer
title_full Structural studies on a glycopeptide from the tree fungus Cyttaria harioti Fischer
title_fullStr Structural studies on a glycopeptide from the tree fungus Cyttaria harioti Fischer
title_full_unstemmed Structural studies on a glycopeptide from the tree fungus Cyttaria harioti Fischer
title_sort structural studies on a glycopeptide from the tree fungus cyttaria harioti fischer
url http://hdl.handle.net/20.500.12110/paper_00086215_v138_n2_p257_Oliva
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