Immobilized 4-aminophenyl 1-thio-β-D-galactofuranoside as a matrix for affinity purification of an exo-β-D-galactofuranosidase

An alternative and fast method for the purification of an exo-β-D-galactofuranosidase has been developed using a 4-aminophenyl 1-thio-β-D-galactofuranoside affinity chromatography system and specific elution with 10 mM D-galactono-1,4-lactone in a salt gradient. A concentrated culture medium from Pe...

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Detalles Bibliográficos
Autores principales:	Miletti, L.C., Marino, C., Mariño, K., De Lederkremer, R.M., Colli, W., Alves, M.J.M.
Formato:	JOUR
Materias:	β-D-Galactofuranose 1-thio-β-D-Galactofuranoside Affinity chromatography exo-β-D-Galactofuranosidase Penicillium fellutanum 4 aminophenyl 1 thio beta dextro galactofuranoside concanavalin a dextro galactono 1,4 lactone enzyme antibody exo beta dextro galactofuranosidase galactofuranosidase glycoprotein immobilized enzyme microbial enzyme sepharose unclassified drug affinity chromatography article controlled study enzyme activity enzyme isolation enzyme kinetics enzyme purification enzyme substrate immunoaffinity chromatography immunoprecipitation nonhuman penicillium pH polyacrylamide gel electrophoresis priority journal technique western blotting beta-Galactosidase Chromatography, Affinity Galactosides Glycoside Hydrolases Hydrogen-Ion Concentration Kinetics Ligands Penicillium
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00086215_v320_n3-4_p176_Miletti
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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