Macrocycle versus podant-type neutral ionophore in potentiometric detection of mucolytic agents following separation by various HPLC modes

Potentiometric detection of lipophilic drugs such as clenbuterol, ambroxol and bromhexine in standard-bore cyano and narrow-bore hybrid, reversed-phase HPLC systems was developed to enable specific determination of these mucolytic agents in some marketed oral-formulations. The macrocycle and podant-...

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Detalles Bibliográficos
Autores principales: Bazylak, G., Nagels, L.J., Monge, M.E.
Formato: JOUR
Materias:
Column liquid chromatography
Lipophilic drugs
Pharmaceutical formulations
Potentiometric detection
acetonitrile
ambroxol
bromhexine
clenbuterol
hexakis(2,3,6,tri o octyl) alpha cyclodextrin
ionophore
macrocyclic compound
mucolytic agent
n,n,n'n' tetracyclohexyloxybis(2 phenyleneoxy)diacetamide
polyvinylchloride
unclassified drug
acidity
aqueous solution
article
electrode
lipophilicity
liquid membrane
potentiometry
priority journal
reversed phase high performance liquid chromatography
separation technique
signal noise ratio
standardization
ultraviolet radiation
waveform
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00095893_v57_n11-12_p757_Bazylak
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Potentiometric detection of lipophilic drugs such as clenbuterol, ambroxol and bromhexine in standard-bore cyano and narrow-bore hybrid, reversed-phase HPLC systems was developed to enable specific determination of these mucolytic agents in some marketed oral-formulations. The macrocycle and podant-type, neutral ionophores, respectively: hexakis(2,3,6-tri-O-octyl)-α-cyclodextrin (CDX) and N,N,N′,N′-tetracyclohexyl-oxybis(o-phenyleneoxy)diacetamide (TOPA), were used to construct PVC-based, liquid membrane electrodes. In acidic aqueous-acetonitrile, mobile phases both ionophores improved the sensitivity of these electrodes for mucolytic agents when compared to single-wavelength UV detection - as well as with the ionophore-free based electrode. In the case of bromhexine, indicating highest lipophilicity (logP 6.40), detection limits of 2.6 × 10-10 and 1.4 × -10 M (injected concentration) were achieved with a signal-to-noise (S/N) ratio of 3 with the TOPA-modified, liquid membrane electrode in the variaus RP-HPLC systems. The results of procedures validated for simultaneous potentiometric determination of clenbuterol and ambroxol in tablets marketed in different countries are also presented.

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