Enzymatic and immunological detection of G protein α-subunits in the pathogenic fungus Candida albicans

GTP stimulation of adenylyl cyclase from the dimorphic pathogenic fungus Candida albicans is greatly enhanced by preincubation of membrane proteins with cholera toxin, NAD and ATP. In the presence of [32P]NAD the toxin catalyzes the covalent incorporation of radioactivity into a menbrane protein of...

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Detalles Bibliográficos
Autores principales: Paveto, C., Montero, L., Passeron, S.
Formato: JOUR
Materias:
Adenylyl cyclase
Candida albicans
G-protein
Hormone response
Peptide antiserum, α-commons
adenosine triphosphate
adenylate cyclase
cholera toxin
guanine nucleotide binding protein
membrane protein
nicotinamide adenine dinucleotide
pertussis toxin
protein subunit
article
candida albicans
nonhuman
priority journal
signal transduction
Adenosine Triphosphate
Adenylate Cyclase
Blotting, Western
Cholera Toxin
GTP-Binding Proteins
Guanosine Triphosphate
Membrane Proteins
NAD
Poly(ADP-ribose) Polymerases
Support, Non-U.S. Gov't
Virulence Factors, Bordetella
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00145793_v311_n1_p51_Paveto
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:GTP stimulation of adenylyl cyclase from the dimorphic pathogenic fungus Candida albicans is greatly enhanced by preincubation of membrane proteins with cholera toxin, NAD and ATP. In the presence of [32P]NAD the toxin catalyzes the covalent incorporation of radioactivity into a menbrane protein of 40 kDa. Pertussis toxin catalyzes the transference of the radioactivity from [32P]NAD to a 32 kDa protein. Two major proteins of 40-42 and 30-32 kDa can also be recognized in Western blots by an anti Gα-common antibody. The results support the idea that G proteins are part of the hormonesensory transduction chain of Candida [(1990) Biochem. Biophys. Res. Commun. 167. 1177-1183]. © 1992.

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