Different porphobilinogen-deaminase forms in wild and mutant strains of saccharomyces cerevisiae. A possible correlation with its segregants behaviour

1. 1. Different porphobilinogen-deaminase (PBG-D) enzyme forms were found for D 27 and D 27/C6 (HEM R+) strains of Saccharomyces cerevisiae. 2. 2. PBG-D was partially purified and chromatographed on Sephadex G-100 in either the presence or absence of a protease inhibitor. For D 27 only one active pe...

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Detalles Bibliográficos
Autores principales: Garcia, S.R.C., Rossetti, M.V., del Carmen Batlle, A.M.
Formato: JOUR
Materias:
endogenous compound
porphobilinogen deaminase
article
fungus mutant
nonhuman
protein variant
saccharomyces cerevisiae
Ammonia-Lyases
Chromatography, Gel
Isoenzymes
Mutation
Saccharomyces cerevisiae
Support, Non-U.S. Gov't
Fungi
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_0020711X_v23_n10_p1019_Garcia
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:1. 1. Different porphobilinogen-deaminase (PBG-D) enzyme forms were found for D 27 and D 27/C6 (HEM R+) strains of Saccharomyces cerevisiae. 2. 2. PBG-D was partially purified and chromatographed on Sephadex G-100 in either the presence or absence of a protease inhibitor. For D 27 only one active peak was observed while for D 27/C6 strain two active peaks were found. 3. 3. A correlation between this differential behaviour and the presence of HEM R+ gene was looked for employing two segregants of one tetrad from D 27 and D 27/C6 mating. © 1991.

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